ATAD5 antibody
Principal name
ATAD5 antibody
Alternative names for ATAD5 antibody
C17orf41, FRAG1, ATPase family AAA domain-containing protein 5, Chromosome fragility-associated gene 1 protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of ATAD5 antibody
- Kit Component:
- - KN301712G1, Atad5 gRNA vector 1 in pCas-Guide vector
- KN301712G2, Atad5 gRNA vector 2 in pCas-Guide vector
- KN301712D, donor vector containing left and right homologous arms and RFP-BSD functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot using ATAD5 polyclonal antibody ( Cat # PAB10222 ) shows detection of a band ~120 kDa corresponding to human ATAD5 ( arrowhead ) in various cell lysates.Lanes contain ~ 5 µg of HeLa nuclear extract ( 1 ), HeLa ( 2 ), A-431( 3 ), Jurkat ( 4 ) and HEK293 ( 5 ) whole cell lysates.After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.The expected molecular weight of ATAD5 is 120 kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa.The 50 kDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide ( datanot shown ).
WB using Anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. Primary antibody was used at 1:500. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319505 | ATAD5 (63-76) antibody |
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| Rabbit polyclonal anti-ELG-1 antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu | E, WB |
0.1 mg /
€515.00
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| OriGene Technologies, Inc. | |||||||