ATF3 antibody
Principal name
ATF3 antibody
Alternative names for ATF3 antibody
Activating transcription factor 3, ATF-3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3
Ncbi ID
NP_001025458.1, 9606, NP_001665, NP_001025458, AAH06322, NM_001674, NM_001040619, NM_001030287, NP_031524.2, NM_012912
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)
Background of ATF3 antibody
This protein binds the cAMP response element (CRE) (consensus: 5\'-GTGACGT[AC][AG]-3\'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
General readings
Sampson,M.J., (2008) Eur. J. Endocrinol. 158 (5), 729-739
Western blot analysis of ATF3 over-expressed 293 cell line, cotransfected with ATF3 Validated Chimera RNAi ( Cat # H00000467-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF3 monoclonal antibody (M01), clone 6B8 (Cat # H00000467-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of ATF3 expression in transfected 293T cell line by ATF3 monoclonal antibody (M01), clone 6B8.Lane 1: ATF3 transfected lysate(20.6 KDa).Lane 2: Non-transfected lysate.
ATF3 monoclonal antibody (M01), clone 6B8 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged ATF3 is approximately 1ng/ml as a capture antibody.
ATF3 monoclonal antibody (M02), clone 8D8 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged ATF3 is approximately 3ng/ml as a capture antibody.
ATF3 monoclonal antibody (M04), clone 8G5 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged ATF3 is approximately 1ng/ml as a capture antibody.
ATF3 monoclonal antibody (M06), clone 7G10 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged ATF3 is approximately 3ng/ml as a capture antibody.
ATF3 monoclonal antibody (M07), clone 6G11 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged ATF3 is approximately 3ng/ml as a capture antibody.
AP07545PU-N ATF3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Prostate at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
Western Blot analysis of ATF3 expression in transfected 293T cell line (H00000467-T01) by ATF3 MaxPab polyclonal antibody.Lane 1: ATF3 transfected lysate(19.91 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ATF3 expression in transfected 293T cell line (H00000467-T01) by ATF3 MaxPab polyclonal antibody.Lane 1: ATF3 transfected lysate(20.60 KDa).Lane 2: Non-transfected lysate.
OVCAR-3; Host: Rabbit. Target Name:ATF3. Sample Tissue:OVCAR-3. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12.; ATF3 antibody - middle region (ARP36556_P050) in OVCAR-3 cells using Western Blot
Human OVCAR-3; WB Suggested Anti-ATF3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: OVCAR-3 cell lysate; ATF3 antibody - middle region (ARP36556_P050) in Human OVCAR-3 cells using Western Blot
Recombinant human ATF3, 1-181 aa
ATF3 Antibody (C-term) (Cat. #AP50284PU-N) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATF3 antibody detected the ATF3 protein (arrow).
Immunohistochemical analysis of ATF3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of ATF3 expression in HeLa (A); mouse heart (B); rat heart (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF3 (RC202897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF3.(1:2000)
Western blot analysis of extracts from RAW264.7 cells, using ATF3 antibody.The lane on the right is treated with the synthesized peptide.
WB of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. The membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
WB of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with
Western blot analysis of extracts of various cell lines, using ATF3 antibody.
Western Blot: ATF3 Antibody [TA336705] - Western blot analysis of ATF3 on UV treated NIH/3T3 cell extracts using TA336705.
Immunohistochemistry: ATF3 Antibody [TA336705] - Analysis of ATF3 in human prostate using Fast Red with hematoxylin counterstain.
WB Suggested Anti-ATF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysate
Host: Rabbit; Target Name:ATF3; Sample Tissue:OVCAR-3; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1 ug/ml; Peptide Concentration: 5ug/ml; Lysate Quantity: 25ug/lane/lane; Gel Concentration: 0.12
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313482 | ATF3 antibody |
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| Rabbit polyclonal anti-ATF3 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA319331 | ATF3 (113-130) antibody |
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| Rabbit polyclonal anti-ATF3 antibody | |||||||
![WB of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. The membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/600-401-493-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Hu | E, WB |
0.1 mg /
€515.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA332447 | ATF3 antibody |
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| Rabbit anti-ATF3 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA336705 | ATF3 antibody |
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| Rabbit Polyclonal ATF3 Antibody | |||||||
![Western Blot: ATF3 Antibody [TA336705] - Western blot analysis of ATF3 on UV treated NIH/3T3 cell extracts using TA336705.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-02935-1-W.jpg) |
Rabbit | Purified | Hu, Mam, Ms, Rt | C, E, P, WB |
0.1 ml /
€450.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA808558 | ATF3 antibody |
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| ATF3 mouse monoclonal antibody, clone OTI5G9 (formerly 5G9) | |||||||
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Mouse | IgG2b | OTI5G9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| AP07545PU-N | ATF3 antibody |
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Rabbit | Aff - Purified | Hu | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP50284PU-N | ATF3 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760366 | ATF3 (N-term HIS tag, transcript variant 3) |
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| Purified recombinant protein of Human activating transcription factor 3 (ATF3), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
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|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419814 | ATF3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY421782 | ATF3 overexpression lysate |
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0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | ||
| LY422248 | ATF3 overexpression lysate |
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0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | ||
| LY425714 | ATF3 overexpression lysate |
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0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. | ||
| LY429075 | ATF3 overexpression lysate |
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212713L2V | Lenti ORF particles, Atf3 (GFP-tagged ORF) - Rat activating transcription factor 3 (Atf3), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR212713L1V | Lenti ORF particles, Atf3 (Myc-DDK-tagged ORF) - Rat activating transcription factor 3 (Atf3), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||
