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ATF6 alpha antibody

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Principal name

ATF6 alpha antibody

Alternative names for ATF6 alpha antibody

ATF-6, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha

SwissProt ID

B2RU98 (Mouse), F6VAN0, P18850 (Human)

Gene ID

22926 (ATF6), 226641 (Atf6)

Ncbi ID

9606, NP_031374.2, NP_031374, 10090, NM_007348

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear, Can (Canine), Rt (Rat), Fi (Fish)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), ChIP assay (ChIP), Flow Cytometry (F)

Background of ATF6 alpha antibody

ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptiol activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen, 2005 & Prywes, 2005). The C-terminus of ATF6 is located in the ER lumen and its N-termil D binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress sigl across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to certain stress conditions, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within the Golgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors, and is referred to as regulated intramembrane proteolysis. The N-termil active ATF6 translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptiol activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in deturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment.

H00022926-B01P - ATF6 alpha
Immunofluorescence of purified MaxPab antibody to ATF6 on HeLa cell. [antibody concentration 10 ug/ml]
H00022926-B01P - ATF6 alpha
Western Blot analysis of ATF6 expression in transfected 293T cell line (H00022926-T01) by ATF6 MaxPab polyclonal antibody.Lane 1: ATF6 transfected lysate(22.22 KDa).Lane 2: Non-transfected lysate.
PAB13179 - ATF6 alpha
Immunocytochemical staining of MCF-7 cells using ATF6 polyclonal antibody ( Cat # PAB13179 ) at 10 µg/ml.
PAB13179 - ATF6 alpha
Western blot analysis of ATF6 in MCF-7 cell lysate with ATF6 polyclonal antibody ( Cat # PAB13179 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
PAB13180 - ATF6 alpha
Immunocytochemistry of ATF6 in A-20 with ATF6 polyclonal antibody ( Cat # PAB13180 ) at 10 µg/ml.
PAB13180 - ATF6 alpha
Western blot analysis of ATF6 in MDA-MB-361 cell lysate with ATF6 polyclonal antibody ( Cat # PAB13180 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
AP08807PU-N - ATF6 alpha
AP08807PU-N staining of ATF6 in Breast tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded).
AP08807PU-N - ATF6 alpha
Western blot analysis of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at 0.5 µg/ml (A) and 1 µg/ml (B).
AP08853PU-N - ATF6 alpha
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
AP17892PU-N - ATF6 alpha
AP17892PU-N ATF6 Antibody (Center) staining of Formalin-Fixed and Paraffin-Embedded Human brain tissue. This antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP17892PU-N - ATF6 alpha
AP17892PU-N ATF6 Antibody (Center) staining of A2058 cell line lysates (35ug/lane) in Western blot analysis. ATF6 (arrow) was detected using the purified Pab.
H00022926-D01 - ATF6 alpha
Immunoprecipitation of ATF6 transfected lysate using anti-ATF6 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ATF6 purified MaxPab mouse polyclonal antibody (B01P) (H00022926-B01P).
ARP31688_P050 - ATF6 alpha
Human HeLa; WB Suggested Anti-ATF6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate. ATF6 is supported by BioGPS gene expression data to be expressed in HeLa.; ATF6 antibody - N-terminal region (ARP31688_P050) in Human HeLa cells using Western Blot
ARP32293_P050 - ATF6 alpha
Mouse adipose; Atf6 antibody - C-terminal region (ARP32293_P050) in Mouse adipose cells using Immunohistochemistry
ARP32293_P050 - ATF6 alpha
Mouse Heart; WB Suggested Anti-Atf6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Mouse Heart; Atf6 antibody - C-terminal region (ARP32293_P050) in Mouse Heart cells using Western Blot
AP23812PU-N - ATF6 alpha
Human Uterus (formalin-fixed, paraffin-embedded) stained with ATF6 antibody AP23812PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM31749PU-N - ATF6 alpha
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP32822PU-N - ATF6 alpha
Staining of HeLa lysate using ATF6 Antibody Cat.-No AP32822PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA306286 - ATF6 alpha
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL.
TA306286 - ATF6 alpha
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/mL.
TA306286 - ATF6 alpha
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.
TA306287 - ATF6 alpha
Western blot analysis of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL.
TA306287 - ATF6 alpha
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/mL.
TA326767 - ATF6 alpha
Western blot analysis of extracts of various cell lines, using ATF6 antibody.
TA326767 - ATF6 alpha
Immunohistochemistry of paraffin-embedded human lung using ATF6 antibody at dilution of 1:200 (200x lens).
TA326767 - ATF6 alpha
Immunohistochemistry of paraffin-embedded human lung cancer using ATF6 antibody at dilution of 1:200 (200x lens).
TA326767 - ATF6 alpha
Immunohistochemistry of paraffin-embedded rat lung using ATF6 antibody at dilution of 1:200 (200x lens).
TA326767 - ATF6 alpha
Immunohistochemistry of paraffin-embedded rat spleen using ATF6 antibody at dilution of 1:200 (200x lens).
TA326767 - ATF6 alpha
Immunofluorescence analysis of A549 cell using ATF6 antibody. Blue: DAPI for nuclear staining
TA330233 - ATF6 alpha
WB Suggested Anti-Atf6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Mouse Heart
TA330233 - ATF6 alpha
Mouse adipose
TA336753 - ATF6 alpha
Western Blot: ATF6 Antibody (70B1413.1) [TA336753] - Analysis of ATF6 in NIH3T3 cell lysate using TA336753 at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kDa observed band; it may be an ATF6 breakdown/
TA336753 - ATF6 alpha
Western Blot: ATF6 Antibody (70B1413.1) [TA336753] - Analysis of ATF6. Lane 1, 293 cells transfected with full-length ATF6.* Lane 2, 293 cells transfected with partial length ATF6 (amino acids 1-373).* Lane 3, Untransfected 293 cells. Western blots were p
TA336753 - ATF6 alpha
Western Blot: ATF6 Antibody (70B1413.1) [TA336753] - Western blot analysis of ATF6 in mouse liver tissue using 3 ug/ml of A TF6 antibody and 0.25 ug/ml of GAPDH antibody. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of tota
TA336753 - ATF6 alpha
Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [TA336753] - ATF6 antibody (10 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human placenta, followed by biotinylated horse anti-mouse IgG secondary antibody, alkaline phosphatas
TA336753 - ATF6 alpha
Flow Cytometry: ATF6 Antibody (70B1413.1) [TA336753] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using ATF6 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
TA336820 - ATF6 alpha
Simple Western: ATF6 Antibody [TA336820] - Simple Western lane view shows a specific band detected for ATF 6 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).
TA336820 - ATF6 alpha
Immunocytochemistry/Immunofluorescence: ATF6 Antibody [TA336820] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
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11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF6 alpha antibody
  ATF6 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ATF6 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+5 additional images

Highly validated antibody!

ATF6 alpha antibody
  Mouse Monoclonal ATF6 Antibody (70B1413.1)  
Western Blot: ATF6 Antibody (70B1413.1) [TA336753] - Analysis of ATF6 in NIH3T3 cell lysate using TA336753 at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kDa observed band; it may be an ATF6 breakdown/ Mouse IgG1 70B1413.1 Purified Fi, Hst, Hu, Ms, Rb, Rt C, ChIP, F, ICC/IF, IP, P, WB
0.1 mg / €380.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

ATF6 alpha (437-449) antibody
Staining of HeLa lysate using ATF6 Antibody Cat.-No AP32822PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por, Rt E, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

ATF6 alpha (C-term) antibody
  Rabbit Polyclonal ATF6 Antibody  
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

ATF6 alpha (N-term) antibody
  Rabbit Polyclonal ATF6 Antibody  
Western blot analysis of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

ATF6 alpha antibody
  Rabbit Polyclonal ATF6 Antibody  
Simple Western: ATF6 Antibody [TA336820] - Simple Western lane view shows a specific band detected for ATF 6 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa). Rabbit Purified Hu ICC/IF, WB
0.1 ml / €395.00
  OriGene Technologies, Inc.
+1 additional image

ATF6 alpha antibody
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3D5 Purified Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH

ATF6 alpha antibody
AP08807PU-N staining of ATF6 in Breast tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

ATF6 alpha antibody
Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

ATF6 alpha (Center) antibody
AP17892PU-N ATF6 Antibody (Center) staining of Formalin-Fixed and Paraffin-Embedded Human brain tissue. This antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

ATF6 alpha (C-term) antibody
Human Uterus (formalin-fixed, paraffin-embedded) stained with ATF6 antibody AP23812PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Elephant, Hu, Rb E, P
50 µg / €425.00
  OriGene Technologies GmbH

11 Item(s)

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11 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (11)
Clonality
  1. Polyclonal (9)
  2. Monoclonal (2)
Target
  1. ATF6 alpha (11)
Property
  1. 437-449 (1)
  2. C-term (2)
  3. Center (1)
  4. N-term (1)
Reactivity
  1. Human (Hu) (11)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (3)
  4. Rabbit (Rb) (2)
  5. Bovine (Bov) (1)
  6. Canine (Can) (1)
  7. Elephant (1)
  8. Fish (Fi) (1)
  9. Hamster (Hst) (1)
  10. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (7)
  3. Paraffin Sections (P) (7)
  4. Enzyme Immunoassay (E) (6)
  5. Frozen sections (C) (1)
  6. ChIP assay (ChIP) (1)
  7. Flow cytometry (F) (1)
  8. Immunoprecipitation (IP) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (5)
Host
  1. Rabbit (7)
  2. Goat (2)
  3. Mouse (2)
Images
  1. 1 image (11)
  2. 3+ images (3)
  3. 5+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Paraffin Sections (P) (5)
  3. Immunofluorescence (IF) (4)
  4. IHC (2)
Manufacturer
  1. OriGene Technologies (11)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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