ATP synthase subunit alpha antibody
Principal name
ATP synthase subunit alpha antibody
Alternative names for ATP synthase subunit alpha antibody
ATP5A1, ATP5A, ATP5AL2, ATPM, F1F0 ATP synthase alpha subunit, Complex V alpha subunit, mitochondrial ATP synthase subunit alpha
SwissProt ID
P07251 (Yeast), P0ABB0 (Ecoli), P15999 (Rat), P25705 (Human), P35381 (Drome), Q03265 (Mouse), Q9XXK1 (Caeel)
Ncbi ID
NP_001001937.1, NP_001001937, NP_004037, NP_031531.1, NM_007505, NM_023093
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of ATP synthase subunit alpha antibody
- Kit Component:
- - KN301787G1, Atp5a1 gRNA vector 1 in pCas-Guide vector
- KN301787G2, Atp5a1 gRNA vector 2 in pCas-Guide vector
- KN301787D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from 293/RAW264.7 cells, using ATP5A1 Antibody. The lane on the right is treated with the synthesized peptide.
ATP5A1 Antibody (C-term) (Cat. #AP50297PU-N) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
ATP5A1 Antibody (C-term) (Cat. #AP50297PU-N) western blot analysis in WiDr,NCI-H460,MDA-MB231 cell line lysates (35µg/lane).This demonstrates the ATP5A1 antibody detected the ATP5A1 protein (arrow).
Immunohistochemical analysis of ATP5A1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of ATP5A1 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Western blot analysis of extracts from RAW264.7 cells and 293 cells, using ATP5A1 antibody.The lane on the right is treated with the synthesized peptide.
TA327766 (0.01 ug/ml) staining of Human (A), fetal Mouse (B), adult Mouse (C) and adult Rat (D) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA327766 (0.01 ug/ml) staining of Pig Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313487 | ATP synthase subunit alpha antibody |
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| Rabbit polyclonal anti-ATP5A1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327766 | ATP synthase subunit alpha antibody |
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| ATP5A1 Goat Polyclonal Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP31337PU-N | ATP synthase subunit alpha antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP50297PU-N | ATP synthase subunit alpha (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314840 | ATP synthase subunit alpha |
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| Recombinant protein of human ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle (ATP5A1), nuclear gene encoding mitochondrial protein, transcript vari | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208769L2V | Lenti ORF particles, Atp5a1 (GFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 (Atp5a1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
| MR208769L1V | Lenti ORF particles, Atp5a1 (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 (Atp5a1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||