ATP synthase subunit d antibody
Principal name
ATP synthase subunit d antibody
Alternative names for ATP synthase subunit d antibody
ATP5H, My032, F1 F0 ATP synthase dubunit d, Complex V subunit d, mitochondria ATP synthase subunit d
Ncbi ID
9606, NP_001003785.1, NP_001003785, NP_006347, NM_027862, NM_019383, NM_001003785
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)
Background of ATP synthase subunit d antibody
- Kit Component:
- - KN207008G1, ATP5H gRNA vector 1 in pCas-Guide vector
- KN207008G2, ATP5H gRNA vector 2 in pCas-Guide vector
- KN207008D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Noh H S., et al. (2004) Brain Res. 129:80-87.Sansanwal P., et al. (2010) J Am Soc Nephrol. 21:272-283.
ATP5H MaxPab polyclonal antibody. Western Blot analysis of ATP5H expression in human liver.
Western Blot analysis of ATP5H expression in transfected 293T cell line (H00010476-T02) by ATP5H MaxPab polyclonal antibody.Lane 1: ATP5H transfected lysate(17.71 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ATP5H expression in transfected 293T cell line (H00010476-T02) by ATP5H MaxPab polyclonal antibody.Lane 1: ATP5H transfected lysate(18.50 KDa).Lane 2: Non-transfected lysate.
Hum. Adult Placenta; Host:Rabbit. Target Name:ATP5H. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; ATP5H antibody - C-terminal region (ARP52160_P050) in Hum. Adult Placenta cells using Western Blot
Human MCF-7; WB Suggested Anti-ATP5H Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate. ATP5H is supported by BioGPS gene expression data to be expressed in MCF7.; ATP5H antibody - C-terminal region (ARP52160_P050) in Human MCF-7 cells using Western Blot
ATP5H antibody (Center) (Cat. #AP50301PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ATP5H Antibody (Center) (Cat. #AP50301PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).
ATP5H Antibody (Center) (Cat. #AP50301PU-N) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-Fixed, Paraffin-Embedded Human Heart stained with ATP5H Antibody Cat.-No AP33219PU-N
Western blot analysis of extracts from HepG2/Jurkat cells, using ATP5H Antibody Cat.-No AP33219PU-N. The lane on the right is treated with the synthesized peptide.
Western blot analysis of ATP5H expression in Hela (A); SHSY5Y (B); Rat muscle (C) whole cell lysates.
Immunohistochemical analysis of ATP5H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of extracts from HepG2 cells and Jurkat cells, using ATP5H antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: ATP5H; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-ATP5H Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysateATP5H is supported by BioGPS gene expression data to be expressed in MCF7
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315059 | ATP synthase subunit d antibody |
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| Rabbit polyclonal anti-ATP5H antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AP33219PU-N | ATP synthase subunit d (111-160) antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP50301PU-N | ATP synthase subunit d (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307908 | ATP synthase subunit d (transcript variant 1) |
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| Recombinant protein of human ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d (ATP5H), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201260L2V | Lenti ORF particles, Atp5h (GFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d (Atp5h), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201260L1V | Lenti ORF particles, Atp5h (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d (Atp5h), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201071L2V | Lenti ORF particles, Atp5h (GFP-tagged ORF) - Rat ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d (Atp5h), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR201071L1V | Lenti ORF particles, Atp5h (Myc-DDK-tagged ORF) - Rat ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d (Atp5h), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
