ATP1B2 antibody
Principal name
ATP1B2 antibody
Alternative names for ATP1B2 antibody
Sodium pump ATPase subunit beta 2, Sodium/potassium-transporting ATPase subunit beta-2, Sodium/potassium-dependent ATPase subunit beta-2
Ncbi ID
9606, NP_038201.1, NP_001669, NM_001678, NP_001669.3, NM_013415
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of ATP1B2 antibody
- Kit Component:
- - KN301773G1, Atp1b2 gRNA vector 1 in pCas-Guide vector
- KN301773G2, Atp1b2 gRNA vector 2 in pCas-Guide vector
- KN301773D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hernando N., et al. (1994). Biophys. Acta 1189:109-111
Detection limit for recombinant GST tagged ATP1B2 is approximately 3ng/ml as a capture antibody.
Mouse Kidney; WB Suggested Anti-Atp1b2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Mouse Kidney; Atp1b2 antibody - N-terminal region (ARP45156_P050) in Mouse Kidney cells using Western Blot
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ATP1B2 Antibody (Center) (Cat. #AP50294PU-N) in K562 cell line lysates (35µg/lane). ATP1B2 (arrow) was detected using the purified Pab.
ATP1B2 Antibody (Center) (Cat. #AP50294PU-N) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (ATP1B2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (ATP1B2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA349694 | ATP1B2 antibody |
||||||
| Rabbit Polyclonal Anti-ATP1B2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| AP50294PU-N | ATP1B2 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP320004 | ATP1B2 |
||||
| Recombinant protein of human ATPase, Na+/K+ transporting, beta 2 polypeptide (ATP1B2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419811 | ATP1B2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203972L2V | Lenti ORF particles, Atp1b2 (GFP-tagged) - Mouse ATPase, Na+/K+ transporting, beta 2 polypeptide (Atp1b2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203972L1V | Lenti ORF particles, Atp1b2 (Myc-DDK-tagged) - Mouse ATPase, Na+/K+ transporting, beta 2 polypeptide (Atp1b2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||