ATP7B antibody
Principal name
ATP7B antibody
Alternative names for ATP7B antibody
Copper pump 2, PWD, WC1, WND, Copper-transporting ATPase 2, Wilson disease-associated protein
Gene ID
540 (ATP7B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of ATP7B antibody
- Kit Component:
- - KN223635G1, ATP7B gRNA vector 1 in pCas-Guide vector
- KN223635G2, ATP7B gRNA vector 2 in pCas-Guide vector
- KN223635D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
ATP7B monoclonal antibody (M01), clone 3E10. Western Blot analysis of ATP7B expression in human colon.
Detection limit for recombinant GST tagged ATP7B is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATP7B is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATP7B is approximately 0.03ng/ml as a capture antibody.
Western blot analysis of ATP7B in 20 µg of mouse brain membrane fraction using ATP7B polyclonal antibody ( Cat # PAB12477 ).
Western blot analysis of ATP7B expression in HepG2 (A); Human lung (B) whole cell lysates.
Immunohistochemical analysis of ATP7B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ATP7B staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunoblot versus rat brain membranes (RBM), rat liver membranes (RLM) and lysates of COS-1 cells transfected with human Copper-transporting ATPase 2 (COS+) or empty vector (COS-) and probed with L62/29 tissue culture supernatant.
Immunoblot versus rat brain membranes (RBM), rat liver membranes (RLM) and lysates of COS-1 cells transfected with human Copper-transporting ATPase 2 (COS+) or empty vector (COS-) and probed with L62/29 tissue culture supernatant.
ATP7b detected in 20 ug of mouse brain membrane fraction
Immunohistochemistry: ATP7b Antibody - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using ATP7B antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using ATP7B antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of ATP7B antibody (C-term) (Cat.# TA324843) in HepG2 cell line lysates (35ug/lane). ATP7B (arrow) was detected using the purified Pab.
ATP7B Antibody (C-term) (Cat.# TA324843) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of ATP7B Antibody (C-term)(Cat#TA324843) with HepG2 cell followed by Alexa Fluoræ´?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ATP7B Antibody (C-term) (Cat. #TA324843) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using ATP7B antibody.
Immunofluorescence analysis of U20S cell using ATP7B antibody. Blue: DAPI for nuclear staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301761 | ATP7B antibody |
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| Rabbit Polyclonal Antibody against ATP7b | |||||||
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Rabbit | IgG | Purified | Hu, Ms | P, WB |
0.1 ml /
€450.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA313380 | ATP7B antibody |
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| Rabbit polyclonal anti-ATP7B antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA324843 | ATP7B (C-term) antibody |
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| Rabbit polyclonal ATP7B Antibody (C-term) | |||||||
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Rabbit | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA326438 | ATP7B antibody |
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| Mouse monoclonal Copper Transporting ATPase2 Antibody | |||||||
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Mouse | IgG1 | S62-29 | Purified | Hu, Ms, Rt |
0.1 mg /
€410.00
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| OriGene Technologies, Inc. | |||||||
| TA332835 | ATP7B antibody |
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| Rabbit anti-ATP7B Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| 73-143 | ATP7B antibody |
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Mouse | IgG1 | L62/29 | Supernatant | Hu, Ms, Rt | C, IP, WB |
5 ml /
€490.00
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| Antibodies Incorporated | |||||||
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| 75-143 | ATP7B antibody |
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Mouse | IgG1 | L62/29 | Purified | Hu, Ms, Rt | C, IP, WB |
0.1 ml /
€570.00
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| Antibodies Incorporated | |||||||
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323635 | ATP7B (transcript variant 1) |
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| Recombinant protein of human ATPase, Cu++ transporting, beta polypeptide (ATP7B), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC223635L2V | Lenti ORF particles, ATP7B (mGFP-tagged) - Human ATPase, Cu++ transporting, beta polypeptide (ATP7B), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€2,680.00
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| OriGene Technologies, Inc. | ||
| RC223635L1V | Lenti ORF particles, ATP7B (Myc-DDK tagged) - Human ATPase, Cu++ transporting, beta polypeptide (ATP7B), transcript variant 1 , 200 uL, >10^7 TU/mL |
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200 µl /
€2,420.00
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| OriGene Technologies, Inc. | ||