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Augurin antibody

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Principal name

Augurin antibody

Alternative names for Augurin antibody

C2orf40, ECRG4

SwissProt ID

Q32KM8 (Bovin), Q7ZXZ6 (Xenla), Q99LS0 (Mouse), Q9H1Z8 (Human)

Gene ID

84417 (C2orf40)

Ncbi ID

NP_115787.1, AAI09386, NM_032411, NP_115787

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F)

Background of Augurin antibody

ECRG4 (esophageal cancer-related gene 4 protein), also known as augurin or C2orf40, is a 148 amino acid secreted protein. Belonging to the augurin family, ECRG4 is thought to be a hormone. It has also been suggested that ECRG4 may act as a tumor suppressor. The gene that encodes ECRG4 maps to human chromosome 2, which consists of 237 million bases encoding over 1,400 genes, making up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. An extremely rare recessive genetic disorder, Alström syndrome is due to mutations in the ALMS1 gene. Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes.

General readings

Su T, Liu H, and Lu S. Cloning and identification of cDNA fragments related to human esophageal cancer. Zhonghua Zhong Liu Za Zhi 1998; 20:254-7.

C11679-1 - Augurin
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using ECRG4 Antibody. The picture on the right is treated with the synthesized peptide.
C11679-1 - Augurin
Immunofluorescence analysis of A549 cells, using ECRG4 Antibody. The picture on the right is treated with the synthesized peptide.
TA502699 - Augurin
Immunofluorescent staining of HeLa cells using anti-C2orf40 mouse monoclonal antibody (TA502699).
TA502699 - Augurin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40.
TA502699 - Augurin
Flow cytometric Analysis of Hela cells, using anti-C2orf40 antibody(TA502699),(Red), compared to a nonspecific negative control antibody,(Blue).
TA502699 - Augurin
Flow cytometric Analysis of Jurkat cells, using anti-C2orf40 antibody(TA502699),(Red), compared to a nonspecific negative control antibody,(Blue).
TA502718 - Augurin
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C2orf40 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
TA502718 - Augurin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40.
TA502718 - Augurin
Flow cytometric Analysis of Hela cells, using anti-C2orf40 antibody(TA502718),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
TA502718 - Augurin
HEK293T cells transfected with either RC206239 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C2orf40 antibody(TA502718), and then analyzed by flow cytometry.
TA502752 - Augurin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40.
TA502752 - Augurin
HEK293T cells transfected with either RC206239 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C2orf40 antibody(TA502752), and then analyzed by flow cytometry.
TA502752 - Augurin
Flow cytometric Analysis of Hela cells, using anti-C2orf40 antibody(TA502752),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
TP306239 - Augurin
TA312430 - Augurin
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using ECRG4 antibody.The picture on the right is treated with the synthesized peptide.
TA312430 - Augurin
Immunofluorescence analysis of A549 cells, using ECRG4 antibody.The picture on the right is treated with the synthesized peptide.
TA320049 - Augurin
Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 ug/mL.
TA320049 - Augurin
Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 ug/mL.
TA350656 - C2orf40
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (C2orf40 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA350656 - C2orf40
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (C2orf40 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
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  2. Highly Validated (2)

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Augurin antibody
  C2orf40 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)  
Immunofluorescent staining of HeLa cells using anti-C2orf40 mouse monoclonal antibody (TA502699). Mouse IgG2b OTI3B10 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

TrueMAB

Augurin antibody
  C2orf40 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)  
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C2orf40 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon). Mouse IgG2b OTI1A10 Purified Hu F, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

TrueMAB

Augurin antibody
  Rabbit polyclonal anti-ECRG4 antibody  
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using ECRG4 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Augurin (C-term) antibody
  Rabbit Polyclonal ECRG4 Antibody  
Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

Augurin antibody
  Rabbit Polyclonal Anti-C2orf40 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (C2orf40 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Augurin antibody
  C2orf40 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40. Mouse IgG2a OTI1F6 Purified Hu F, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Augurin
  Recombinant protein of human chromosome 2 open reading frame 40 (C2orf40)  
Augurin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

C2orf40 overexpression lysate
C2orf40 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

8 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (6)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (3)
  2. Polyclonal (3)
Target
  1. Augurin (7)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (3)
  3. Flow cytometry (F) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  5. Paraffin Sections (P) (2)
Presentation
  1. Purified (5)
  2. Aff - Purified (1)
Host
  1. Mouse (3)
  2. Rabbit (3)
Images
  1. 1 image (7)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Flow cytometry (F) (3)
  3. Immunofluorescence (IF) (2)
  4. IHC (2)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (8)
Service
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