AVEN antibody
Principal name
AVEN antibody
Alternative names for AVEN antibody
PDCD12, Cell death regulator Aven, Programmed cell death 12
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of AVEN antibody
- Kit Component:
- - KN301876G1, Aven gRNA vector 1 in pCas-Guide vector
- KN301876G2, Aven gRNA vector 2 in pCas-Guide vector
- KN301876D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Figueroa B Jr, et al: (2004) Biotechnol Bioeng. 85(6), 589-600.
Cheng EH, et al: (2000) Mol Cell. 6(1), 31-40.
Skin: Formalin-Fixed Paraffin-Embedded (FFPE)
Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE)
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis.
AVEN monoclonal antibody (M08), clone 2B10. Western Blot analysis of AVEN expression in NIH/3T3(Cat # L018V1 ).
AVEN monoclonal antibody (M08), clone 2B10. Western Blot analysis of AVEN expression in Jurkat ( Cat # L017V1 ).
AVEN monoclonal antibody (M08), clone 2B10. Western Blot analysis of AVEN expression in PC-12(Cat # L012V1 ).
Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Western blot analysis: The extracts of mouse kidney (50 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The extract of mouse kidney ( 50 µg ) was resolved by SDS-PAGE and probed with AVEN monoclonal antibody, clone 3G4 ( Cat # MAB2053 ) ( 1:1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemistry of AVEN in human spleen tissue with AVEN polyclonal antibody ( Cat # PAB13091 ) at 5 µg/ml.
Western blot analysis of AVEN in Raji cell lysate with AVEN polyclonal antibody ( Cat # PAB13091 ) at 1 µg/ml in ( A ) the presence and ( B ) the absence of blocking peptide.
Immunohistochemistry of AVEN in human spleen tissue with AVEN polyclonal antibody ( Cat # PAB13093 ) at 5 µg/ml.
Western blot analysis of AVEN in Raji cell lysate with AVEN polyclonal antibody ( Cat # PAB13093 ) at 1 µg/ml in ( A ) the presence and ( B ) the absence of blocking peptide.
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
Immunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 ug/ml.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/mL.
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
mmunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 ug/ml.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/mL.
Western blot analysis of extracts from LOVO cells and COS-7 cells, using AVEN antibody.The lane on the right is treated with the synthesized peptide.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AVEN Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AVEN Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane 1-2: Raji cells, Hela cells, Primary antibody: AVEN Antibody at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AVEN Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using AVEN Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM09049PU-N | AVEN antibody |
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 |
Mouse | IgG2a | 3G4 | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| AM09049PU-S | AVEN antibody |
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|
Mouse | IgG2a | 3G4 | Purified | Hu, Ms | E, P, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA306068 | AVEN (C-term) antibody |
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| Rabbit Polyclonal Aven Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA306070 | AVEN (N-term) antibody |
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| Rabbit Polyclonal Aven Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA315642 | AVEN antibody |
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| Rabbit polyclonal anti-AVEN antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322258 | AVEN antibody |
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| Anti-AVEN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA322259 | AVEN antibody |
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| Anti-AVEN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP07274PU-N | AVEN antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP07657PU-N | AVEN antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303707 | AVEN |
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| Recombinant protein of human apoptosis, caspase activation inhibitor (AVEN) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412513 | AVEN overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |