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B3GAT1 antibody

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Principal name

B3GAT1 antibody

Alternative names for B3GAT1 antibody

GLCATP, GLCUATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

SwissProt ID

O35789 (Rat), Q5CB03 (Canfa), Q9CW73 (Mouse), Q9P2W7 (Human)

Gene ID

27087 (B3GAT1), 117108 (B3gat1)

Ncbi ID

NP_061114, NP_061114.2, NP_005801, NP_005801.3, ABM86204.1, NM_054025, NM_018644, NM_054003

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of B3GAT1 antibody

CD57 is a terminally sulfated glycan carbohydrate epitope (glycoepitope) first discovered on human natural killer cells in 1981. CD57 is also referred to as CD57 antigen. Historically, the term CD57 has been used in immunology for defining lymphocytic subpopulations. CD57 antibody positive lymphocytes are typically either T or NK cells and are commonly found within the germinal centers of the spleen, lymph nodes and tonsils. CD57 antibody positivity in T lymphocytes has long been used as a marker of in vitro replicative senescence (clonal exhaustion). CD57 antibody positive T lymphocytes have a high susceptibility to activation-induced death. CD57 upregulation or unusual antibody positivity patterns have been identified in diseases including autoimmunity, chronic infections, and malignancies. Increased CD57 antibody positivity has also been associated with aging, allogenic transplantation, and even physical and psychological stress.

AP50325PU-N - CD57 / HNK1
Western blot analysis of B3GAT1 Antibody (N-term) (Cat. #AP50325PU-N) in K562 cell line lysates (35µg/lane). B3GAT1 (arrow) was detected using the purified Pab.
AP50325PU-N - CD57 / HNK1
B3GAT1 Antibody (N-term) (Cat. #AP50325PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP32143PU-N - CD57 / B3GAT1
AP32143PU-N (0.1 µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
AM32815SU-S - CD57 / HNK1
FACS analysis of CD57 on 106 Human lymphocytes using 25 µl of CD57 Antibody Clone HNK-1
AM32815SU-S - CD57 / HNK1
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD57 Antibody Cat.-No AM32815SU (Clone HNK-1).
AM33131SU-L - CD57 / HNK1
FACS analysis of CD57 on lymphocytes using CD57 Antibody (Clone NK-1).
AM33131SU-L - CD57 / HNK1
Formalin-fixed, paraffin-embedded human spleen stained with CD57 Antibody (Clone NK-1).
AM33253SU-S - CD57 / HNK1
Formalin-Fixed, Paraffin-Embedded Human spleen stained with CD57 Antibody Cat.-No AM33253SU (Clone SPM129).
AM33253SU-S - CD57 / HNK1
FACS analysis of CD57 on lymphocytes using CD57 Antibody (Clone SPM129).
AM33254SU-S - CD57 / HNK1
Formalin-Fixed, Paraffin-Embedded Human spleen stained with CD57 Antibody Cat.-No AM33254SU (Clone SPM527).
AM33254SU-S - CD57 / HNK1
FACS analysis of CD57 on lymphocytes using CD57 Antibody (Clone SPM527).
DB 216-0.1 - CD57 / HNK1
Formalin-fixed and paraffin-embedded poorly differentiated human neuroblastoma tissue stained with anti-CD57 (DB 216).
CPA8379-50Assays - B3GAT1
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD57 Antibody-FITC labled.
CPA8451-50Assays - B3GAT1
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD57 Antibody-PE labled.
CPA8543-100ul - B3GAT1
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD57 Antibody; followed by anti-mouse IgG PE.
TA309582 - B3GAT1
Immunohistochemistry: CD57 Antibody - IHC analysis of CD57 in mouse brain using DAB with hematoxylin counterstain.
TA309582 - B3GAT1
Immunocytochemistry: CD57 Antibody - CD57 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 594 (red).
TA310043 - B3GAT1
Western blot analysis of SGC cells (Lanes 1 and 2: 40ug lysate), mouse liver lysate (Lane 3: 78ug lysate) and mouse brain lysate (Lanes 4 and 5: 84ug lysate) with anti-CD57 antibody diluated at 1:1000. (Predicted and observed size: 38kDa)
TA327647 - B3GAT1
Immunohistochemistry staining of Paraffin Tonsil tissue by CD57 antibody (dilution: 1:100 - 1:500; visualization of staining: Membranous)
TA331374 - B3GAT1
WB Suggested Anti-B3GAT1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Lung
TA336743 - B3GAT1
Immunohistochemistry: CD57 Antibody [TA336743] - IHC analysis of CD57 in mouse brain using DAB with hematoxylin counterstain.
TA336743 - B3GAT1
Immunocytochemistry/Immunofluorescence: CD57 Antibody [TA336743] - CD57 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
TA351058 - B3GAT1
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human chromaffin cell tumor tissue, Primary antibody: (B3GAT1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
TA351058 - B3GAT1
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (B3GAT1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA351058 - B3GAT1
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (B3GAT1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

12 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

B3GAT1 antibody
  Rabbit Polyclonal CD57 Antibody  
Immunohistochemistry: CD57 Antibody - IHC analysis of CD57 in mouse brain using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

B3GAT1 antibody
  Rabbit anti-B3GAT1 polyclonal antibody  
Western blot analysis of SGC cells (Lanes 1 and 2: 40ug lysate), mouse liver lysate (Lane 3: 78ug lysate) and mouse brain lysate (Lanes 4 and 5: 84ug lysate) with anti-CD57 antibody diluated at 1:1000. (Predicted and observed size: 38kDa) Rabbit IgG Purified Hu WB
0.1 ml / €195.00
  OriGene Technologies, Inc.

B3GAT1 antibody
  Mouse Monoclonal B3GAT1 (CD57) Antibody  
Immunohistochemistry staining of Paraffin Tonsil tissue by CD57 antibody (dilution: 1:100 - 1:500; visualization of staining: Membranous) Mouse IgM NK1 Purified Hu P
1 ml / €435.00
  OriGene Technologies, Inc.

B3GAT1 antibody
  Rabbit Polyclonal beta-1,3-Glucuronyltransferase 1/B3GAT1/CD57 Antibody  
Immunohistochemistry: CD57 Antibody [TA336743] - IHC analysis of CD57 in mouse brain using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P
0.1 ml / €395.00
  OriGene Technologies, Inc.
+1 additional image

B3GAT1 antibody
  Rabbit Polyclonal Anti-B3GAT1 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human chromaffin cell tumor tissue, Primary antibody: (B3GAT1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

CD57 / B3GAT1 antibody
AP32143PU-N (0.1 µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / €380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

CD57 / HNK1 (N-term) antibody
Western blot analysis of B3GAT1 Antibody (N-term) (Cat. #AP50325PU-N) in K562 cell line lysates (35µg/lane). B3GAT1 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.    

B3GAT1 overexpression lysate
B3GAT1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

B3GAT1 overexpression lysate
B3GAT1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

B3GAT1 overexpression lysate
B3GAT1 overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, B3gat1 (GFP-tagged ORF) - Rat beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1), 200 uL, >10^7 TU/mL
Lenti ORF particles, B3gat1 (GFP-tagged ORF) - Rat beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, B3gat1 (Myc-DDK-tagged ORF) - Rat beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1), 200 uL, >10^7 TU/mL
Lenti ORF particles, B3gat1 (Myc-DDK-tagged ORF) - Rat beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

12 Item(s)

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12 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (3)
  2. Primary Antibody (7)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (1)
Target
  1. B3GAT1 (5)
  2. CD57 / B3GAT1 (1)
  3. CD57 / HNK1 (1)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (3)
  4. Canine (Can) (1)
  5. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (3)
  3. Frozen sections (C) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Enzyme Immunoassay (E) (1)
  6. Flow cytometry (F) (1)
Presentation
  1. Purified (5)
  2. Aff - Purified (2)
Host
  1. Rabbit (5)
  2. Goat (1)
  3. Mouse (1)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. IHC (4)
  2. Western blot / Immunoblot (WB) (4)
  3. Immunofluorescence (IF) (2)
  4. Flow cytometry (F) (1)
Manufacturer
  1. OriGene Technologies (12)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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