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B4GALT3 antibody

Principal name

B4GALT3 antibody

Alternative names for B4GALT3 antibody

Beta-1,4-GalTase 3, Beta4Gal-T3, b4Gal-T3, Beta-1,4-galactosyltransferase 3, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3

SwissProt ID

O60512 (Human), Q5EA87 (Bovin), Q6P768 (Rat), Q91YY2 (Mouse)

Gene ID

8703 (B4GALT3), 57370 (B4galt3), 494342

Ncbi ID

9606, NP_001186802.1, NP_003770, NM_003779, NP_065604.2, NM_001009539

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of B4GALT3 antibody

Several oligosaccharide structures and protein glycoconjugate types are found in nature. Homologous glycosyltransferase (GT) gene families catalyze the formation of glycosidic linkages. The β-1,3 galactosyltransferase(β3GalT) gene family encodes a set of type II transmembrane glycoproteins that are catalytically diverse and use different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine ) to catalyze the addition of an activated monosaccharide to a terminal lactose. The protein coding sequences for β-1,3-Gal-T genes comprise a single exon and are distantly related to the Drosophila Brainiac gene. The β-1,4-galactosyltransferase (β4GalT) gene family encodes type II membrane-bound glycoproteins that show exclusive specificity for the donor substrate, UDP-galactose. β-1,4Gal-T genes transfer galactose in a β-1,4 linkage to similar acceptor sugars; each gene has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures.

Western Blot analysis of B4GALT3 expression in transfected 293T cell line (H00008703-T01) by B4GALT3 MaxPab polyclonal antibody.Lane 1: B4GALT3 transfected lysate(43.23 KDa).Lane 2: Non-transfected lysate.

H00008703-B01

H00008703-B01P

Immunohistochemistry(IHC) analyzes of β-1,4-Gal-T3 antibody (Cat.-No.: AP01385PU-N) in paraffin-embedded human liver carcinoma tissue.

AP01385PU-N

Western blot (WB) analysis of β-1,4-Gal-T3 antibody (Cat.-No.: AP01385PU-N) in extracts from Jurkat cells.

AP01385PU-N

Human 721_B; WB Suggested Anti-B4GALT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. B4GALT3 is supported by BioGPS gene expression data to be expressed in 721_B.; B4GALT3 antibody - middle region (ARP45533_P050) in Human 721_B cells using Western Blot

ARP45533_P050

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using B4GALT3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat/HeLa/HepG2 cells, using B4GALT3 Antibody. The lane on the right is treated with the synthesized peptide.

Human 293T; WB Suggested Anti-B4GALT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: 293T cell lysate. B4GALT3 is supported by BioGPS gene expression data to be expressed in HEK293T.; B4GALT3 antibody - N-terminal region (ARP45532_P050) in Human 293T cells using Western Blot

ARP45532_P050

Immunohistochemical analysis of B4GALT3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of B4GALT3 expression in Jurkat (A); HL60 (B); HeLa (C); COLO205 (D) whole cell lysates.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B4GALT3 (RC202909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B4GALT3.(1:2000)

TA808622

Western blot analysis of extracts from HepG2 cells, HeLa cells and Jurkat cells, using B4GALT3 antibody.The lane on the right is treated with the synthesized peptide.

TA314795

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using B4GALT3 antibody.The picture on the right is treated with the synthesized peptide.

TA314795

WB Suggested Anti-B4GALT3 Antibody Titration: 0.2-1 ug/ml; Positive Control: 293T cell lysate.B4GALT3 is supported by BioGPS gene expression data to be expressed in HEK293T

TA338207

WB Suggested Anti-B4GALT3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysate.B4GALT3 is supported by BioGPS gene expression data to be expressed in 721_B

TA338208

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA314795	B4GALT3 antibody
	Rabbit polyclonal anti-B4GALT3 antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA808622	B4GALT3 antibody
	B4GALT3 mouse monoclonal antibody, clone OTI1G9 (formerly 1G9)
	Mouse	IgG2a	OTI1G9	Purified	Hu	WB	0.1 ml / €360.00
							OriGene Technologies, Inc.

AP01385PU-N	B4GALT3 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP721112	B4GALT3 (transcript variant 2)
	Purified recombinant protein of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3 (B4GALT3), transcript variant 2
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	10 µg / €299.00
				OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR201013L2V	Lenti ORF particles, B4galt3 (GFP-tagged ORF) - Rat UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 (B4galt3), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR201013L1V	Lenti ORF particles, B4galt3 (Myc-DDK-tagged ORF) - Rat UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 (B4galt3), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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