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BACH1 antibody

Principal name

BACH1 antibody

Alternative names for BACH1 antibody

HA2303, Transcription regulator protein BACH1, BTB and CNC homolog 1, basic leucine zipper transcription factor 1

SwissProt ID

O14867 (Human), P97302 (Mouse)

Gene ID

571 (BACH1), 12013 (Bach1)

Ncbi ID

NP_001177.1, NP_001177, NP_001011545, 9606, ABZ91995.1, NP_996749, NM_001186, NP_031546.1, NM_007520

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F)

Background of BACH1 antibody

Members of the small Maf family (MafK, MafF, and MafG) are basic region leucine zipper (bZip) proteins that can function as transcriptional activators or repressors. They dimerize with other proteins and bind DNA to either represse or activate transcription depending on the dimer compositions. BACH1 and BACH2, heterodimerization partners of MafK, are members of a novel family of BTB/POZ-basic region leucine zipper (bzip) factors. BACH1 and BACH2 have significant similarity to each other in BTB domain and Cap‘n’ collar-type bZip domain but are otherwise divergent. BACH1 appears ubiquitous, whereas BACH2 is restricted to monocytes, neuronal cells and is abundantly expressed in the early stages of B-cell differentiation. BACH2, a 841 amino acid polypeptide, is an Nrf2-related transcription repressor and a tissue-specific partner of the Maf oncoprotein family. In culture cells, BACH2 is localized to the cytoplasm through its C-terminal cytoplasmic localization signal (CLS). Oxidative stressors aborted the CLS activity and induce nuclear accumulation of BACH2, which mediates nucleocytoplasmic communciation to couple oxidative stress and transcription repression in mammalian cells. BACH2 heterodimerizes with MAZR through its BTB/POZ domain to activate transcription. BACH2 also plays an important role in the regulation of B cell development.

General readings

Litman,R., et al., (2005) Cancer Cell 8 (3), 255-265

Western blot (WB) analysis of BACH1 antibody (Cat.-No.: AP06634PU-N) in extracts from HeLa cells.

AP06634PU-N

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)

AP07549PU-N

BACH1 Antibody (C-term) (Cat. #AP50333PU-N) western blot analysis in ZR-75-1 cell line lysates (35µg/lane).This demonstrates the BACH1 antibody detected the BACH1 protein (arrow).

AP50333PU-N

Human Jurkat; WB Suggested Anti-BACH1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; BACH1 antibody - C-terminal region (ARP38012_T100) in Human Jurkat cells using Western Blot

ARP38012_T100

Immunofluorescence analysis of HeLa cells, using BACH1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HepG2 cells, using BACH1 Antibody. The lane on the right is treated with the synthesized peptide.

BACH1 monoclonal antibody (M02), clone 1B8 Western Blot analysis of BACH1 expression in NIH/3T3 ( Cat # L018V1 ).

H00000571-M02

Detection limit for recombinant GST tagged BACH1 is approximately 0.03ng/ml as a capture antibody.

H00000571-M02

Western blot using BACH1 polyclonal antibody ( Cat # PAB10027 ) shows detection of a band at~105 kDa ( lane 1 ) corresponding to human BACH1 present in a 293 whole cell lysate ( arrowhead ).Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction.Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1,000.Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye^TM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).IRDye^TM800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc.

PAB10027

Immunohistochemical analysis of BACH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of BACH1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of BACH1 expression in HEK293T (A); MCF7 (B); K562 (C); HeLa (D) whole cell lysates.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BACH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504484)

TA504484

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504484)

TA504484

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BACH1 (RC221628, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BACH1.

TA504484

HEK293T cells transfected with either RC221628 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BACH1 antibody(TA504484), and then analyzed by flow cytometry.

TA504484

TP309275

TP321628

Western blot analysis of extracts from HeLa cells, HepG2 cells and 293 cells, using BACH1 antibody.The lane on the right is treated with the synthesized peptide.

TA312525

Immunofluorescence analysis of HeLa cells, using BACH1 antibody.The picture on the right is treated with the synthesized peptide.

TA312525

WB Suggested Anti-BACH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate

TA335183

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA504484	BACH1 antibody
	BACH1 mouse monoclonal antibody, clone OTI4E11 (formerly 4E11)
	Mouse	IgG1	OTI4E11	Purified	Hu	F, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+3 additional images	Top Selling product! Highly validated antibody!
AP06634PU-N	BACH1 antibody
	Rabbit			Aff - Purified	Hu	ICC/IF, P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
TA312525	BACH1 antibody
	Rabbit polyclonal anti-BACH1 antibody
	Rabbit	IgG		Purified	Hu	E, ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
AP07549PU-N	BACH1 (Isoform 1) (92-104) antibody
	Rabbit			Aff - Purified	Hu	E, ICC/IF, P, WB	50 µg / €425.00
							OriGene Technologies GmbH
AP50333PU-N	BACH1 (C-term) antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP309275	BACH1 (transcript variant 2)
	Recombinant protein of human BTB and CNC homology 1, basic leucine zipper transcription factor 1 (BACH1), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP321628	BACH1 (transcript variant 1)
	Recombinant protein of human BTB and CNC homology 1, basic leucine zipper transcription factor 1 (BACH1), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY400475	BACH1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210390L2V	Lenti ORF particles, Bach1 (GFP-tagged) - Mouse BTB and CNC homology 1 (Bach1), 200 uL, >10^7 TU/mL
		200 µl / €1,530.00
		OriGene Technologies, Inc.

MR210390L1V	Lenti ORF particles, Bach1 (Myc-DDK-tagged) - Mouse BTB and CNC homology 1 (Bach1), 200 uL, >10^7 TU/mL
		200 µl / €1,370.00
		OriGene Technologies, Inc.

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