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BAG2 antibody

Principal name

BAG2 antibody

Alternative names for BAG2 antibody

BAG-2, BAG family molecular chaperone regulator 2, Bcl-2-associated athanogene 2

SwissProt ID

O95816 (Human), Q3ZBG5 (Bovin), Q91YN9 (Mouse)

Gene ID

9532 (BAG2), 213539 (Bag2)

Ncbi ID

9606, NP_004273, NP_004273.1, NP_663367.1, NM_145392, NM_004282

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of BAG2 antibody

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

General readings

Dai Q, Qian SB, Li HH, McDonough H, Borchers C, Huang D, Takayama S, Younger JM, Ren HY, Cyr DM, Patterson C.
Regulation of the cytoplasmic quality control protein degradation pathway by BAG2.
J Biol Chem. 2005 Nov 18;280(46):38673-81. Epub 2005 Sep 16.
PMID: 16169850

BAG2 monoclonal antibody (M01), clone 6E12 Western Blot analysis of BAG2 expression in HeLa ( Cat # L013V1 ).

H00009532-M01

Immunofluorescence confocal microscopy of BAG2. Using BAG2 polyclonal antibody ( Cat # PAB0329 ) at 1 : 2000. HeLa cells stably transfected with Myc-CHIP ( carboxyl terminus of Hsp70-interacting protein ) and growing on coverslips were left untreated ( A ) or heat shocked at 42 degrees for 30 min ( B ) . Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al ( 2005 ) for additional information on HeLa cells stably transfected with Myc-CHIP.

PAB0329

Western blot analysis of BAG2. Using BAG2 polyclonal antibody ( Cat # PAB0329 ) at 1 : 2000. HeLa stably transfected Myc-tagged CHIP ( carboxyl terminus of Hsp70-interacting protein ) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by western blot analysis of BAG2. This experiment was done to show the colocalization of CHIP and Bag2. Please refer to Dai et al ( 2005 ) for additional information on HeLa cells stably transfected with Myc-CHIP.

PAB0329

BAG2 polyclonal antibody ( Cat # PAB6054 ) staining ( 0.3 µg/ml ) of HeLa lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6054

Human Kidney; Rabbit Anti-BAG2 Antibody. Catalog Number: ARP42177. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; BAG2 antibody - C-terminal region (ARP42177_T100) in Human Kidney cells using Immunohistochemistry

ARP42177_T100

siRUVBL transfected human Saos2; . Sample Type :. Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human Saos2 cells . Primary Antibody Dilution :. 1:1000 . Secondary Antibody:. Anti-rabbit-HRP . Secondary Antibody Dilution:. 1:3000 . Color/Signal Descriptions:. BAG2 . Gene Name:. Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. . Submitted by:.; BAG2 antibody - C-terminal region (ARP42177_T100) in siRUVBL transfected human Saos2 cells using Western Blot

ARP42177_T100

Human Jurkat; WB Suggested Anti-BAG2 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. BAG2 is supported by BioGPS gene expression data to be expressed in Jurkat.; BAG2 antibody - C-terminal region (ARP42177_T100) in Human Jurkat cells using Western Blot

ARP42177_T100

Western Blot analysis of BAG2 expression in transfected 293T cell line (H00009532-T01) by BAG2 MaxPab polyclonal antibody.Lane 1: BAG2 transfected lysate(23.21 KDa).Lane 2: Non-transfected lysate.

H00009532-B01P

Immunoprecipitation of BAG2 transfected lysate using anti-BAG2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BAG2 MaxPab mouse polyclonal antibody (B01) (H00009532-B01).

H00009532-D01

Recombinant human BAG2, 1-211 aa, His-tagged

TA302728 (0.3µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA302728

Primary Antibodies

Catalog No.	Host	Pres.	React.	Applications
TA302728	BAG2 (C-term) antibody
	Goat Polyclonal Antibody against BAG2
	Goat	Aff - Purified	Hu	WB	0.1 mg / €360.00
					OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR202268L2V	Lenti ORF particles, Bag2 (GFP-tagged) - Mouse BCL2-associated athanogene 2 (Bag2), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR202268L1V	Lenti ORF particles, Bag2 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 2 (Bag2), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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