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BAG4 antibody

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Principal name

BAG4 antibody

Alternative names for BAG4 antibody

BAG-4, SODD, BAG family molecular chaperone regulator 4, Bcl-2-associated athanogene 4, Silencer of death domains

SwissProt ID

O95429 (Human), Q8CI61 (Mouse)

Gene ID

9530 (BAG4), 67384 (Bag4)

Ncbi ID

NP_004865.1, 9606, NP_004865, NM_004874, NP_080397.1, NM_026121

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of BAG4 antibody

Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands. Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation. SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.
SODD (Silencer of Death Domain) is an important regulator of TNF induced apoptotic pathway. It associates with TNF-R1 to prevent constitutive activation of the signaling events downstream. It dissociates from the TNF-R1 after TNF binds TNF-R1 to allow the activation of apoptotic pathway. It is expressed ubiquitously in human tissues.

General readings

Annunziata CM, Kleinberg L, Davidson B, Berner A, Gius D, Tchabo N, Steinberg SM, Kohn EC.
BAG-4/SODD and associated antiapoptotic proteins are linked to aggressiveness of epithelial ovarian cancer.
Clin Cancer Res. 2007 Nov 15;13(22 Pt 1):6585-92
PMID: 18006758

SP1301 - BAG4
Immunocytochemical staining of Hela cells with Rabbit anti SODD (SP1301)
SP1301 - BAG4
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in theabsence (1,2) or presence (3,4) of blocking peptide with SP1301.
AP07659PU-N - BAG4
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum.
PAB0383 - BAG4
Western blot analysis of SODD in HeLa cell lysate using BAG4 polyclonal antibody ( Cat # PAB0383 ) at 0.5 µg/ml.
H00009530-M02 - BAG4
Western Blot analysis of BAG4 expression in transfected 293T cell line by BAG4 monoclonal antibody (M02), clone 2G9.Lane 1: BAG4 transfected lysate(49.6 KDa).Lane 2: Non-transfected lysate.
H00009530-M02 - BAG4
Detection limit for recombinant GST tagged BAG4 is approximately 3ng/ml as a capture antibody.
H00009530-B01P - BAG4
Immunoperoxidase of purified MaxPab antibody to BAG4 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
H00009530-B01P - BAG4
BAG4 MaxPab polyclonal antibody. Western Blot analysis of BAG4 expression in human pancreas.
H00009530-B01P - BAG4
Western Blot analysis of BAG4 expression in transfected 293T cell line (H00009530-T01) by BAG4 MaxPab polyclonal antibody.Lane 1: BAG4 transfected lysate(50.27 KDa).Lane 2: Non-transfected lysate.
PAB10362 - BAG4
Western blot analysis using BAG4 polyclonal antibody ( Cat # PAB10362 ) on HeLa (lanes 1, 3) and THP-1 (lanes 2, 4) whole cell lysates in the absense (lanes 1, 2) and presense (lanes 3, 4) of blocking peptide.
H00009530-D01 - BAG4
Western Blot analysis of BAG4 expression in transfected 293T cell line (H00009530-T02) by BAG4 MaxPab polyclonal antibody.Lane 1: BAG4 transfected lysate(49.60 KDa).Lane 2: Non-transfected lysate.
H00009530-D01 - BAG4
Immunoprecipitation of BAG4 transfected lysate using anti-BAG4 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BAG4 MaxPab mouse polyclonal antibody (B01) (H00009530-B01).
AP50336PU-N - BAG4
BAG4 Antibody (C-term) (Cat. #AP50336PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the BAG4 antibody detected the BAG4 protein (arrow).
CPA2401-100ul - BAG4
Immunohistochemical analysis of BAG4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2401-100ul - BAG4
Western blot analysis of BAG4 expression in HeLa (A); MOLT4 (B); THP1 (C) whole cell lysates.
TA590615 - BAG4
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590615 - BAG4
ELISA: BAG4 Antibody
TP306235 - BAG4
TA305759 - BAG4
TA305759 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA306005 - BAG4
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.
TA306005 - BAG4
Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 ug/mL.
TA306005 - BAG4
Immunofluorescence of SODD in Hela cells with SODD antibody at 20 ug/mL.
TA312929 - BAG4
Western blot analysis of extracts from K562 cells, using BAG4 antibody.The lane on the right is treated with the synthesized peptide.

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG4 antibody
  Goat Anti-SODD Antibody  
TA305759 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

BAG4 antibody
  Rabbit Polyclonal SODD Antibody  
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

BAG4 antibody
  Rabbit polyclonal anti-BAG4 antibody  
Western blot analysis of extracts from K562 cells, using BAG4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

BAG4 antibody
  Rabbit Polyclonal Anti-BAG4 rabbit polyclonal antibody  
BAG4 Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

BAG4 antibody
  Rabbit Polyclonal BAG4 Antibody  
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu, Rt E, P
0.1 mg / €409.00
  OriGene Technologies, Inc.
+1 additional image

BAG4 antibody
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

BAG4 (C-term) antibody
BAG4 Antibody (C-term) (Cat. #AP50336PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the BAG4 antibody detected the BAG4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BAG4
  Recombinant protein of human BCL2-associated athanogene 4 (BAG4)  
BAG4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

BAG4 overexpression lysate
BAG4 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Bag4 (GFP-tagged) - Mouse BCL2-associated athanogene 4 (Bag4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Bag4 (GFP-tagged) - Mouse BCL2-associated athanogene 4 (Bag4), 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Bag4 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 4 (Bag4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Bag4 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 4 (Bag4), 200 uL, >10^7 TU/mL  
200 µl / €1,010.00
  OriGene Technologies, Inc.
 

11 Item(s)

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Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (7)
  4. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (7)
Target
  1. BAG4 (8)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Enzyme Immunoassay (E) (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. Paraffin Sections (P) (2)
Presentation
  1. Purified (5)
  2. Aff - Purified (2)
Host
  1. Rabbit (6)
  2. Goat (1)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (2)
  3. Enzyme Immunoassay (E) (1)
  4. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (11)
Service
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