BAG5 antibody
Principal name
BAG5 antibody
Alternative names for BAG5 antibody
BAG-5, KIAA0873, BAG family molecular chaperone regulator 5, Bcl-2-associated athanogene 5
Ncbi ID
9606, NP_001015048, NM_001015049, NP_001015048.1, NP_004864, NP_081680.1, NM_027404, NM_001008526, NM_004873
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), EPair for Elisa (EPair)
Background of BAG5 antibody
BAG5 is a member of the BAG1 related protein family. BAG1 is an anti apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL 2, Raf protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. A BAG domain near the C terminus, may bind and inhibit the chaperone activity of Hsc70/Hsp70.
It has been hypothesized that the BAG5 protein will induce the death of nigral neurons through its predicted interaction with hsp70, which will cause increased protein aggregation and cell death by disinhibition of hsp70’s anti apoptotic function. It is believed that BAG5 will play an important role in the mechanisms of neuronal death. BAG5 may also be of interest due to its possible role as a modulator of the hsp70/hsp40 chaperone axis or its possible interaction and coordination of localization/modulation of other BAG containing proteins via BAG-BAG
heterodimerization.
General readings
1 Takayama S, Xie Z, Reed JC (Feb 1999). "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators". J Biol Chem 274 (2): 781–6.
2 Kalia SK, Lee S, Smith PD, Liu L, Crocker SJ, Thorarinsdottir TE, Glover JR, Fon EA, Park DS, Lozano AM (Dec 2004). "BAG5 inhibits parkin and enhances dopaminergic neuron degeneration". Neuron 44 (6): 931–45.
3 "Entrez Gene: BAG5 BCL2-associated athanogene 5"
BAG5 monoclonal antibody (M01), clone 2E2 Western Blot analysis of BAG5 expression in Jurkat ( Cat # L017V1 ).
Immunofluorescence of purified MaxPab antibody to BAG5 on HeLa cell. [antibody concentration 10 ug/ml]
BAG5 MaxPab polyclonal antibody. Western Blot analysis of BAG5 expression in human spleen.
Western Blot analysis of BAG5 expression in transfected 293T cell line (H00009529-T01) by BAG5 MaxPab polyclonal antibody.Lane 1: BAG5 transfected lysate(49.17 KDa).Lane 2: Non-transfected lysate.
BAG5 MaxPab polyclonal antibody. Western Blot analysis of BAG5 expression in HeLa.
Figure 1. Staining BAG5 in Breast by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Formalin-fixed, paraffin-embedded human tissues stained for BAG5 expression. Using BAG5 polyclonal antibody ( Cat # PAB0332 ) at 1 : 2000. A. normal placenta. B. breast cancer. C. normal spleen. normal cervix epithelium. Hematoxylin-eosin counterstain.
BAG5 monoclonal antibody (M02), clone 1E3. Western Blot analysis of BAG5 expression in human spleen.
BAG5 monoclonal antibody (M02), clone 1E3. Western Blot analysis of BAG5 expression in HepG2(Cat # L019V1 ).
BAG5 monoclonal antibody (M02), clone 1E3. Western Blot analysis of BAG5 expression in human spleen.
BAG5 monoclonal antibody (M02), clone 1E3. Western Blot analysis of BAG5 expression in HepG2(Cat # L019V1 ).
Immunofluorescence of purified MaxPab antibody to BAG5 on HeLa cell. [antibody concentration 10 ug/ml]
BAG5 MaxPab polyclonal antibody. Western Blot analysis of BAG5 expression in human spleen.
Western Blot analysis of BAG5 expression in transfected 293T cell line (H00009529-T02) by BAG5 MaxPab polyclonal antibody.Lane 1: BAG5 transfected lysate(49.17 KDa).Lane 2: Non-transfected lysate.
BAG5 MaxPab polyclonal antibody. Western Blot analysis of BAG5 expression in HeLa.
BAG5 MaxPab rabbit polyclonal antibody. Western Blot analysis of BAG5 expression in mouse liver.
BAG5 MaxPab rabbit polyclonal antibody. Western Blot analysis of BAG5 expression in human kidney.
Western Blot analysis of BAG5 expression in transfected 293T cell line (H00009529-T02) by BAG5 MaxPab polyclonal antibody.Lane 1: BAG5 transfected lysate(51.20 KDa).Lane 2: Non-transfected lysate.
AP31064PU-N BAG5 antibody staining of nuclear HeLa lysate at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Fetal liver; WB Suggested Anti-BAG5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Liver; BAG5 antibody - C-terminal region (ARP61996_P050) in Human Fetal liver cells using Western Blot
siRUVBL1 transfected human Saos2; . Sample Type :. Lane 1: 20ug siRUVBL1 transfected human Saos2 cells Lane 2: 20ug untransfected human Saos2 cells . Primary Antibody Dilution :. 1:1000 . Secondary Antibody:. Anti-rabbit-HRP . Secondary Antibody Dilution:. 1:3000 . Color/Signal Descriptions:. BAG5 . Gene Name:. Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. . Submitted by:.; BAG5 antibody - C-terminal region (ARP61996_P050) in siRUVBL1 transfected human Saos2 cells using Western Blot
Human Hela; Host:Rabbit. Target Name:BAG5. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. BAG5 is supported by BioGPS gene expression data to be expressed in HeLa.; BAG5 antibody - C-terminal region (ARP61996_P050) in Human Hela cells using Western Blot
Human HeLa; WB Suggested Anti-BAG5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell. BAG5 is supported by BioGPS gene expression data to be expressed in HeLa.; BAG5 antibody - N-terminal region (ARP61997_P050) in Human HeLa cells using Western Blot
BAG5 Antibody (N-term) (Cat. #AP50337PU-N) western blot analysis in Jurkat cell line lysates (35µg/lane).This demonstrates the BAG5 antibody detected the BAG5 protein (arrow).
Western blot analysis of BAGE5 expression in HeLa (A); A549 (B); MOLT4 (C) whole cell lysates.
TA305758 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30 ug of whole cell lysate). A: Hela. B: Molt-4 . 10% SDS PAGE. TA308752 diluted at 1:1000.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5(TA308752) antibody at 1:200 dilution.
Western blot analysis of extracts from HepG2 cells, using BAG5 Antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 51 kDa . Positive control: A549, Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Hela cell lysate Lane3: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)
Predicted band size: 51 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 20 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31064PU-N | BAG5 (N-term) antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA305758 | BAG5 antibody |
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| Goat Anti-BAG5 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA308752 | BAG5 antibody |
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| Rabbit polyclonal antibody to BAG5 (BCL2-associated athanogene 5) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
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|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA314927 | BAG5 antibody |
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| Rabbit polyclonal anti-BAG5 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA323441 | BAG5 antibody |
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| Anti-BAG5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA323442 | BAG5 antibody |
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| Anti-BAG5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP05164PU-N | BAG5 antibody |
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 |
Rabbit | Purified | Rt | P, WB |
0.1 mg /
€400.00
|
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| OriGene Technologies GmbH | |||||||
| AP07872PU-N | BAG5 antibody |
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 |
Rabbit | Purified | Hu | P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP50337PU-N | BAG5 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN208123 | BAG5 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208123BN | BAG5 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208123LP | BAG5 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208123RB | BAG5 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308518 | BAG5 (transcript variant 2) |
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| Recombinant protein of human BCL2-associated athanogene 5 (BAG5), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP312765 | BAG5 (transcript variant 3) |
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| Recombinant protein of human BCL2-associated athanogene 5 (BAG5), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207130L2V | Lenti ORF particles, Bag5 (GFP-tagged) - Mouse BCL2-associated athanogene 5 (Bag5), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
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| OriGene Technologies, Inc. | ||
| MR207130L1V | Lenti ORF particles, Bag5 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 5 (Bag5), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,000.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201099L2V | Lenti ORF particles, Bag5 (GFP-tagged ORF) - Rat BCL2-associated athanogene 5 (Bag5), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| RR201099L1V | Lenti ORF particles, Bag5 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene 5 (Bag5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,000.00
|
|
| OriGene Technologies, Inc. | ||
