BAI1 antibody
Principal name
BAI1 antibody
Alternative names for BAI1 antibody
BAI-1, Brain-specific angiogenesis inhibitor 1
Ncbi ID
NP_001693.2, 9606, AAI56201.1, NM_001702, NP_778156.2, NM_174991
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of BAI1 antibody
The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-termil tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large. Many domains are localized to the N-terminus; various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-1. The C-termil tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins. Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain. Following autocleavage, the N-termil fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects. At the mR level, all BaIs are expressed in fetal and adult human brain. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain. These receptors are implicated in various diseases and disorders such as primary glioma, pulmory adenocarcinomas, gastric and colorectal cancers.
Brain: Formalin-Fixed Paraffin-Embedded (FFPE)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BAI1 polyclonal antibody ( Cat # PAB4057 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemistry (IHC) analyzes of BAI-1 antibody (Cat.-No.: AP01307PU-N) in paraffin-embedded human brain.
Western blot (WB) analysis of BAI-1 antibody (Cat.-No.: AP01307PU-N) in extracts from Jurkat treated with HepG2.
A. Staining of mouse brain using BAI1 polyclonal antibody ( Cat # PAB12475 ). B. Blocking of staining using control peptide BAI1 polyclonal antibody ( Cat # PAB12475 ).
Western blot analysis of BAI1 expression in HepG2 (A); human brain (B) whole cell lysates.
Immunofluorescent analysis of BAI1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: BAI1 Antibody - A. Staining of mouse brain using anti-BAI1. B. Blocking of staining using control peptide.
Western blot analysis of extracts from Jurkat cells, using BAI1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BAI1 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF-7 cells, using BAI1 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1-2. Anti-BAI1 (extracellular) antibody, (1:200). 3-4. Anti-BAI1 (extracellular) antibody, preincubated with the control peptide antigen.
Western blot analysis of human HL-60 promyelocytic leukemia cell lysates: 1. Anti-BAI1 (extracellular) antibody, (1:200). 2. Anti-BAI1 (extracellular) antibody, preincubated with the control peptide antigen.
Expression of BAI1 in mouse olfactory bulb. Immunohistochemical staining of mouse perfusion-fixed olfactory bulb frozen sections using Anti-BAI1 (extracellular) antibody, (1:200). A. BAI1 (green) is expressed in astrocyte-like cells (arrows). B. Double-staining of BAI1 (green) and glial fibrillary acidic protein (red) reveals expression of BAI1 in a subset of astrocytes. Nuclear staining of cells using the DNA dye DAPI (blue).
Indirect flow cytometry analysis of live intact human HL-60 promyelocytic leukemia cell line:black line, Unstained cells + goat-anti-rabbit-AlexaFluor-488 secondary antibody. green line, Cells + Anti-BAI1 (extracellular) antibody, (1:20) + goat-anti-rabbit-AlexaFluor-488 secondary antibody.
Anti-BAI1 antibody IHC of human brain, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BAI1 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BAI1 antibody IHC of human prostate, benign prostatic hyperplasia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BAI1 antibody IHC of human brain, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BAI1 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BAI1 antibody IHC of human prostate, benign prostatic hyperplasia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BAI1 antibody IHC of human brain, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot analysis of BAI1 expression in 293T (A); mouse brain (B); rat brain (C) whole cell lysates.
Immunohistochemical analysis of BAI1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (BAI1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (BAI1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309583 | BAI1 antibody |
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| Rabbit Polyclonal BAI1 Antibody | |||||||
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Rabbit | Purified | Hu, Ms | P |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| TA313161 | BAI1 antibody |
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| Rabbit polyclonal anti-BAI1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA328720 | BAI1 antibody |
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| Rabbit Polyclonal Anti-BAI1 (extracellular) | |||||||
 |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.2 ml /
€585.00
|
||
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA341036 | BAI1 (C-term) antibody |
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| Rabbit Polyclonal Anti-BAI1 Antibody (C-Terminus) | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341037 | BAI1 (C-term) antibody |
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| Rabbit Polyclonal Anti-BAI1 Antibody (C-Terminus) | |||||||
|
Rabbit | Aff - Purified | Hu, Ms | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341038 | BAI1 (C-term) antibody |
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| Rabbit Polyclonal Anti-BAI1 Antibody (C-Terminus) | |||||||
|
Rabbit | Aff - Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA351000 | BAI1 antibody |
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| Rabbit Polyclonal Anti-BAI1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP01307PU-N | BAI1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP06996PU-N | BAI1 (N-term) antibody |
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 |
Rabbit | Aff - Purified | Bov, Elephant, Hu, Mky | E, P |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419802 | ADGRB1 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC224196L2V | Lenti ORF particles, ADGRB1 (mGFP-tagged) - Human brain-specific angiogenesis inhibitor 1 (BAI1), 200 uL, >10^7 TU/mL |
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200 µl /
€2,680.00
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|
| OriGene Technologies, Inc. | ||
| RC224196L1V | Lenti ORF particles, ADGRB1 (Myc-DDK tagged) - Human brain-specific angiogenesis inhibitor 1 (BAI1) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,420.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR215473L2V | Lenti ORF particles, Bai1 (GFP-tagged) - Mouse brain-specific angiogenesis inhibitor 1 (Bai1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,710.00
|
|
| OriGene Technologies, Inc. | ||
| MR215473L1V | Lenti ORF particles, Bai1 (Myc-DDK-tagged) - Mouse brain-specific angiogenesis inhibitor 1 (Bai1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,440.00
|
|
| OriGene Technologies, Inc. | ||