Bamacan antibody
Principal name
Bamacan antibody
Alternative names for Bamacan antibody
Chondroitin sulfate proteoglycan 6, SMC3, BAM, BMH, CSPG6, SMC3L1, hCAP, Structural maintenance of chromosomes protein 3, Chromosome-associated polypeptide, Basement membrane-associated chondroitin proteoglycan
Ncbi ID
9606, NP_005436, NP_005436.1, NP_031816.2, BC057345, NM_005445
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P)
Background of Bamacan antibody
- Kit Component:
- - KN316297G1, Smc3 gRNA vector 1 in pCas-Guide vector
- KN316297G2, Smc3 gRNA vector 2 in pCas-Guide vector
- KN316297D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of purified MaxPab antibody to CSPG6 on HeLa cell. [antibody concentration 10 ug/ml]
CSPG6 MaxPab polyclonal antibody. Western Blot analysis of CSPG6 expression in human colon.
Western Blot analysis of SMC3 expression in transfected 293T cell line (H00009126-T01) by SMC3 MaxPab polyclonal antibody.Lane 1: CSPG6 transfected lysate(133.87 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SMC3 expression in transfected 293T cell line by CSPG6 monoclonal antibody (M02), clone 2F11.Lane 1: SMC3 transfected lysate(141.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged CSPG6 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to CSPG6 on HeLa cell. [antibody concentration 10 ug/ml]
CSPG6 MaxPab polyclonal antibody. Western Blot analysis of CSPG6 expression in human colon.
Western Blot analysis of SMC3 expression in transfected 293T cell line (H00009126-T01) by SMC3 MaxPab polyclonal antibody.Lane 1: CSPG6 transfected lysate(133.87 KDa).Lane 2: Non-transfected lysate.
Human Testis; Testis; SMC3 antibody - C-terminal region (ARP47590_P050) in Human Testis cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-SMC3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that SMC3 is expressed in HepG2.; SMC3 antibody - C-terminal region (ARP47590_P050) in Human HepG2 cells using Western Blot
Immunoprecipitation of SMC3 transfected lysate using anti-SMC3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CSPG6 MaxPab mouse polyclonal antibody (B01) (H00009126-B01).
WB Suggested Anti-SMC3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate.There is BioGPS gene expression data showing that SMC3 is expressed in HepG2
Testis
WB Suggested Anti-SMC3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: COLO205 cell lysate.There is BioGPS gene expression data showing that SMC3 is expressed in COLO205
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against yeast Smc3K112/113ac. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:16,900.
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209999L2V | Lenti ORF particles, Smc3 (GFP-tagged) - Mouse structural maintenace of chromosomes 3 (cDNA clone MGC:67063 IMAGE:6811961), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,470.00
|
|
| OriGene Technologies, Inc. | ||
| MR209999L1V | Lenti ORF particles, Smc3 (Myc-DDK-tagged) - Mouse structural maintenace of chromosomes 3 (cDNA clone MGC:67063 IMAGE:6811961), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||