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BARD1 antibody

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Principal name

BARD1 antibody

Alternative names for BARD1 antibody

BARD-1, BRCA1-associated RING domain protein 1

SwissProt ID

O70445 (Mouse), Q99728 (Human), Q9QZH2 (Rat)

Gene ID

580 (BARD1), 12021 (Bard1), 64557

Ncbi ID

9606, NP_000456.2, NP_000456, NP_031551.1, NM_022622, NM_000465

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of BARD1 antibody

Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is an ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.

H00000580-M01 - BARD1
Detection limit for recombinant GST tagged BARD1 is approximately 3ng/ml as a capture antibody.
AP07899PU-N - BARD1
Staining BARD1 in Skin by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
AP07899PU-N - BARD1
Staining BARD1 in Skeletal Muscle by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
AP20481PU-N - BARD1
Western blot analysis of BARD1 Antibody Cat.-No.- AP20481PU-N in extracts from HeLa cells at 1/500 dilution.
C13026-1 - BARD1
Western blot analysis of extracts from HeLa cells, using BARD1 Antibody. The lane on the right is treated with the synthesized peptide.
AP50339PU-N - BARD1
BARD1 Antibody (N-term) (Cat. #AP50339PU-N) western blot analysis in WiDr cell line lysates (35µg/lane).This demonstrates the BARD1 antibody detected the BARD1 protein (arrow).
CPA3142-100ul - BARD1
Immunohistochemical analysis of BARD1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3142-100ul - BARD1
Western blot analysis of BARD1 expression in MCF7 (A); HeLa (B) whole cell lysates.
TA313499 - BARD1
Western blot analysis of extracts from HeLa cells, using BARD1 antibody.The lane on the right is treated with the synthesized peptide.
TA313499 - BARD1
Figure from citation: Co-immunoprecipitation Westernblot analysis of proteins from different cell lines using BRCA1 pulldown and probing withanti-BRCA1, PALB2, BARD1, and BRCA2 antibodies. View Citation

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BARD1 antibody
  Rabbit polyclonal anti-BARD1 antibody  
Western blot analysis of extracts from HeLa cells, using BARD1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

BARD1 (108-124) antibody
Staining BARD1 in Skin by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

BARD1 antibody
<b>Western blot</b> analysis of BARD1 Antibody Cat.-No.- AP20481PU-N in extracts from HeLa cells at 1/500 dilution. Rabbit Aff - Purified Hu P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

BARD1 (N-term) antibody
BARD1 Antibody (N-term) (Cat. #AP50339PU-N) western blot analysis in WiDr cell line lysates (35µg/lane).This demonstrates the BARD1 antibody detected the BARD1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Bard1 (Myc-DDK-tagged ORF) - Rat BRCA1 associated RING domain 1 (Bard1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Bard1 (Myc-DDK-tagged ORF) - Rat BRCA1 associated RING domain 1 (Bard1), 200 uL, >10^7 TU/mL  
200 µl / €1,400.00
  OriGene Technologies, Inc.
 

5 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (4)
  2. Rat Lenti ORF Particle (1)
Clonality
  1. Polyclonal (4)
Target
  1. BARD1 (4)
Property
  1. 108-124 (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (4)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (2)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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