BCAT2 antibody
Principal name
BCAT2 antibody
Alternative names for BCAT2 antibody
BCATM, BCT2, ECA40, BCAT(m), Branched-chain-amino-acid aminotransferase mitochondrial
Ncbi ID
9606, NP_001158245, NP_001158245.1, NP_001181, NP_033867.1, NM_009737, NM_001164773
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E)
Background of BCAT2 antibody
- Kit Component:
- - KN302086G1, Bcat2 gRNA vector 1 in pCas-Guide vector
- KN302086G2, Bcat2 gRNA vector 2 in pCas-Guide vector
- KN302086D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Coles SJ. et al. (2009) Biochemistry Jan 27;48(3):645-56.Yennawar N.H.et al. (2007) J. Biol. Chem. 281:39660-39671
BCAT2 MaxPab polyclonal antibody. Western Blot analysis of BCAT2 expression in human kidney.
Western Blot analysis of BCAT2 expression in transfected 293T cell line (H00000587-T01) by BCAT2 MaxPab polyclonal antibody.Lane 1: BCAT2 transfected lysate(43.12 KDa).Lane 2: Non-transfected lysate.
BCAT2 Antibody (C-term) (Cat. #AP50351PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BCAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of BCAT2 (arrow) using rabbit polyclonal BCAT2 Antibody (C-term) (Cat. #AP50351PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCAT2 gene.
Sample (30 ug of whole cell lysate). A: A549. B: H1299. C: HCT116. D: MCF-7. 10% SDS PAGE. TA307893 diluted at 1:500.
Sample (30 ug of whole cell lysate). . A:NIH-3T3. 10% SDS PAGE. . TA307893 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2(TA307893) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2(TA307893) antibody at 1:100 dilution.
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Human placenta tissue, NIH/3T3 cells, Raji cells, hepG2 cells, Primary antibody: (BCAT2 Antibody) at dilution 1/312.5, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (BCAT2 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (BCAT2 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA307893 | BCAT2 antibody |
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| Rabbit Polyclonal antibody to BCAT2 (branched chain aminotransferase 2, mitochondrial) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA349705 | BCAT2 antibody |
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| Rabbit Polyclonal Anti-BCAT2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP50351PU-N | BCAT2 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300625 | BCAT2 |
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| Recombinant protein of human branched chain aminotransferase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206133L2V | Lenti ORF particles, Bcat2 (GFP-tagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||