Bcl-2 antibody
Principal name
Bcl-2 antibody
Alternative names for Bcl-2 antibody
BCL2, Bcl-2 alpha, Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2
SwissProt ID
O02718 (Bovin), P10415 (Human), P10417 (Mouse), P49950 (Rat), Q00709 (Chick), Q6R755 (Canfa)
Ncbi ID
NP_000624, NP_000624.2, 9606, 10090, NP_058689.1, AAH27258, NM_009741, NM_177410, NM_016993, NM_000633
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT)
Background of Bcl-2 antibody
Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membranebound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2’s ability to promote cell survival.
Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (Ref.2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (Ref.3)
Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, gamma-irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection.
General readings
1. Bartoszewski R et al. Mangiferin has an additive effect on the apoptotic properties of hesperidin in Cyclopia sp. tea extracts. PLoS One 9:e92128 (2014). WB ; Human .
2. Adams SM et al. Soy isoflavones genistein and daidzein exert anti-apoptotic actions via a selective ER-mediated mechanism in neurons following HIV-1 Tat(1-86) exposure. PLoS One 7:e37540 (2012). ELISA ; Rat .
3. Fujita H et al. Novel method for measuring active tension generation by C2C12 myotube using UV-crosslinked collagen film. Biotechnol Bioeng 106:482-9 (2010). WB ; Mouse .
Human kidney; BCL2 antibody - N-terminal region (AVARP01005_P050) in Human kidney cells using Immunohistochemistry
Human Placenta; WB Suggested Anti-BCL2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; BCL2 antibody - N-terminal region (AVARP01005_P050) in Human Placenta cells using Western Blot
Western blot analysis of Bcl-2 in HL-60 cell lysate.
Staining of Paraffin embedded human tonsil with Mouse anti Human Bcl-2 Antibody Cat.-No SM1414P
Western blot analysis of Jurkat T cell leukemic whole cell lysate probed with Mouse anti Human Bcl-2 Antibody Cat.-No SM1414P followed by HRP conjugated Goat anti Mouse IgG secondary antibody, visualized using chemiluminescence
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL2 Antibody (#AP02600PU) (Left) or the same antibody preincubated with blocking peptide (Right).
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using BCL2 Antibody (#AP02600PU).
Figure 3. Western blot analysis of extract from Hela, A2780 and 293 cells using BCL2 Antibody (#AP02600PU).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 antibody
Western Blot analysis of extracts from MCF cells using BCL-2 antibody and the same antibody preincubated with blocking peptide
Immunohistochemistry analysis of Bcl-2 antibody (Cat.-No AP06028PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Bcl-2 antibody (Cat.-No.: AP06028PU-N) at 1/500 dilution
Lane1:MCF-7 cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:Raw264.7 cell lysate
Lane1:MCF-7 cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:Raw264.7 cell lysate
Western blot (WB) analysis of Bcl-2 antibody (Cat.-No.: AP06497PU-N) at 1/500 dilution
Lane1: MCF-7 whole cell lysate treated with PMA
Lane2: Mouse brain tissue lysate
Lane3: Rat kidney tissue lysate
Lane1: MCF-7 whole cell lysate treated with PMA
Lane2: Mouse brain tissue lysate
Lane3: Rat kidney tissue lysate
Immunohistochemistry (IHC) analyzes of BCL-2 antibody (Cat.-No.: AP06497PU-N) in paraffin-embedded human colon carcinoma tissue.
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE)
Formalin-fixed paraffin-embedded section of a human prostate cancer tissue array stained for BCL2 expression. Using BCL2 polyclonal antibody ( Cat # PAB0236 ) at 1 : 2000. Hematoxylin-eosin counterstain.
Immunoblot analysis of HL-60 cell lysate.
Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 ( GFP-Nur77/dDBD ) by using BCL2 polyclonal antibody ( Cat # PAB2440 ) for immunoprecipitation ( IP ) and a different BCL2 antibody for western blot. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 ( GFP-Nur77/dDBD ) using BCL2 polyclonal antibody ( Cat # PAB2440 ) in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
BCL2 polyclonal antibody ( Cat # PAB6666 ) staining ( 1 µg/ml ) of human lymph node lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunoblot analysis of cell lysates. Lane 1 : HL-60 cell lysate. Lane 2 : Jurkat T cell lysate.
Immunohistochemistry analysis of p-BCL-2 (pSer70) Antibody Cat.-No AP01536PU-N in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of p-Bcl-2 Antibody Cat.-No AP01536PU-N (pSer70) at 1/500 dilution.
Lane 1: Hela cell lysate treated with colchicine.
Lane 2: Raw264.7 cell lysate treated with colchicine.
Lane 3: PC12 cell lysate treated with colchicine.
Lane 1: Hela cell lysate treated with colchicine.
Lane 2: Raw264.7 cell lysate treated with colchicine.
Lane 3: PC12 cell lysate treated with colchicine.
Western blot (WB) analysis of p-BCL-2 Antibody Cat.-No AP01536PU-N.
Immunohistochemistry (IHC) analysis of p-BCL-2 (pThr56) antibody Cat.-No.: AP01537PU-N in paraffin-embedded human breast carcinoma tissue.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 BH3 Domain Antibody (Cat.#AP11295PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Left panel: Analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP11295PU-N antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel: Same analysis using AP11295PU-N in Flow Cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
Bcl-2, 1-211 aa human, His-tagged, recombinant, E.coli: 15% SDS-PAGE (3 µg)
Human tonsil stained with Anti-Bcl-2 alpha antibody
The whole cell lysate derived from UV treated Raji cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by BCL2 ( phospho T129 ) polyclonal antibody ( Cat # PAB12608 ) at 1 : 500. An immunoreactive band around ~ 26 kDa is observed.
The whole cell lysate derived from UV treated Raji cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by BCL2 ( phospho S70 ) polyclonal antibody ( Cat # PAB12609 ) at 1 : 500. An immunoreactive band around ~ 26 kDa is observed.
The whole cell lysate derived from UV treated Raji cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Phospho-BCL2 T56 polyclonal antibody ( Cat # PAB12610 ) at 1 : 500. An immunoreactive band around ~26 kDa is observed.
Immunohistochemical staining of human kidneyusing BCL2 polyclonal antibody ( Cat # PAB13118 ) at 2 µg/ml.
Western blot analysis of BCL2 in Daudi cell lysates with BCL2 polyclonal antibody ( Cat # PAB13118 ) at ( A ) 1, ( B ) 2, and ( C ) 4 µg/ml.
Immunocytochemistry of BCL2 in A-20 cells with BCL2 polyclonal antibody ( Cat # PAB13119 ) at 2 µg/ml.
Western blot analysis of BCL2 in A-20 cell lysates with BCL2 polyclonal antibody ( Cat # PAB13119 ) at ( A ) 1, ( B ) 2, and ( C ) 4 µg/ml.
Western blot analysis of BCL2 expression in HL-60 cell lysate.
Immunohistochemistry (IHC) analyzes of Bcl-2 antibody (Cat.-No.: AP21081PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of Bcl-2 antibody (Cat.-No.: AP21081PU-N) in extracts from HUVEC cells.
Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with monoclonal anti-human Bcl-2 antibody (1:500) with Texas Red (Red).
ICC/IF analysis of Bcl - 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Bcl - 2 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green)
Flow cytometry analysis of Bcl - 2 in HeLa cell line, staining at 2 - 5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Western blot analysis:Cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis of extracts from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody. The lane on the right is treated with the synthesized peptide.
Human Jurkat; WB Suggested Anti-BCL2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; BCL2 antibody - middle region (ARP58872_P050) in Human Jurkat cells using Western Blot
Lane 1: Rat Heart tissue LysateLane 2: Rat spleen muscle tissue LysateLane 3: Rat small intestine tissue LysateLane 4: Rat liver tissue Lysate
Formalin-Fixed, Paraffin-Embedded Human reactive lymph node stained with Bcl-2 antibody Cat.-No. AM10050SU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic and membrane staining of mantle B-cells cells and perifollicular T-cells and no staining of reactive B-cells in the germinal centers.
Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 mouse mAb with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using BCL-2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).
Western blot analysis of Bcl-2 expression in Jurkat (1), Raji (2), WR19L (3) and PC12 using AM26425AF-N.
Flow Cytometric analysis of Bcl-2 expression in Jurkat cells (left) and WR19L cells (right). Open histograms Indicate the reaction of isotypic control to the cells. Shaded Histograms indicate the reaction of AM26425RP-N to the cells.*The cells were permeabilizedwith digitonin.
Representative pictures of Bcl2 expression in HEK293 cells, visualized with clonal rabbit anti-Bcl2 monospecific antibody (clone P22-A). Primary antibody dilution - 1:200.
Western blot analysis of bcl-2 protein in protein extract from HEK293 cells (A) 100 μg, (B) 200 μg of total protein loaded. His6 tag-fusion recombinant human bcl-2 protein was tested under the same conditions at the concentrations of 100ng (C), 200ng (D).
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with Bcl-2 Antibody Cat.-No AM32832PU (Clone 8C8). Note cell membrane and cytoplasmic staining.
Western blot analysis of Bcl-2 in Human A) HeLa, B) A549 and C) MCF7 lysate using Bcl-2 Antibody Cat.-No AM32832PU (Clone 8C8).
C57BL/6 splenocytes intracellular stained with FITC conjugated Bcl-2 Antibody Cat.-No AM05217FC-N (Clone BCL/10C4) (cells were treated with Foxp3 Fix/Perm buffer, and then permeabilized with Permeabilization Wash Buffer).
C57BL/6 splenocytes intracellularly stained with PE conjuagted Bcl-2 Antibody Cat.-No AM05217RP (Clone BCL/10C4) (cells were treated with Foxp3 Fix/Perm buffer and then permeabilized with Permeabilization Wash Buffer)
Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
Formalin-Paraffin non-Hodgkin's lymphoma stained with Bcl-2 Antibody (Clone 100/D5) Cat.-No AM33245PU. Note nuclear membrane & cytoplasmic staining.
Western blot analysis of Bcl-2 in human skin using Bcl-2 Antibody (Clone 100/D5) Cat.-No AM33245PU
Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 Antibody (Clone 100/D5) Cat.-No AM33245PU (red) and isotype control (green).
Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Antibody Cat.-No AM50163PU (Clone SPM117). Note nuclear membrane & cytoplasmic staining.
Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 Antibody Cat.-No AM50163PU (Clone SPM117)(red) and isotype control (green).
Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Antibody Cat.-No AM50164PU (Clone SPM530). Note nuclear membrane & cytoplasmic staining.
Intracellular staining of Bcl-2 in Jurkat cells using Antibody Cat.-No AM50164PU (Clone SPM530) (red) and isotype control (green).
Formalin-Paraffin non-Hodgkin's lymphoma stained with Bcl-2 Antibody Cat.-No AM33344PU (Clone 124). Note nuclear membrane & cytoplasmic staining.
Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 Antibody Cat.-No AM33344PU (Clone 124) (red) and isotype control (green).
Western blot analysis of Bcl-2 in Human skin using Bcl-2 Antibody Cat.-No AM33344PU (Clone 124).
Formalin-Paraffin non-Hodgkin's lymphoma stained with Bcl-2 Antibody Cat.-No AM50201PU (Clone 100/D5 + 124). Note nuclear membrane & cytoplasmic staining.
Western blot analysis of Bcl-2 in Human skin using Bcl-2 Antibody Cat.-No AM50201PU (Clone 100/D5 + 124).
Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 Antibody Cat.-No AM50201PU (Clone 100/D5 + 124) (red) and isotype control (green).
Western blot analysis of extracts from HeLa cells treated with Nocodazole using Phospho-BCL-2 (Ser87) antibody AP55894PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of BCL2 (pT69) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of BCL2 (pT69) expression in HepG2 UV-treated (A); HeLa (B); HL60 (C); THP1 (D); Jurkat paclitaxel-treated (E); NIH3T3 H2O2-treated (F); PC12 H2O2-treated (G) whole cell lysates.
Immunohistochemical analysis of BCL2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of BCL2 expression in MCF7 (A); mouse brain (B); rat kidney (C) whole cell lysates.
Immunohistochemical analysis of BCL2 (pS70) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of BCL2 (pS70) expression in Jurkat (A); THP1 (B); A2780 (C) whole cell lysates.
ELISA: BCL2 Antibody
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803003)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803003)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCL2 monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806589)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806589)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCL2 monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806591)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806592)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806593)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCL2 monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806623)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCL2 monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806639)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCL2 monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical analysis of BCL2 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of BCL2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of BCL2 expression in MCF7 (A); Raw264.7 (B); NIH3T3 (C) whole cell lysates.
TA302955 staining (1µg/ml) of Human Lymph Node lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 ug/ml.
Immunofluorescence of Bcl-2 in Human Kidney cells with Bcl-2 antibody at 10 ug/mL.
Western blot analysis of Bcl-2 in A-20 cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 ug/mL.
Immunocytochemistry of Bcl-2 in A20 cells with Bcl-2 antibody at 2 ug/mL.
Anti-BCL2 / Bcl-2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Intracellular staining of mouse splenocytes with Anti-Mouse/Rat Bcl-2 FITC. Autofluorescence is shown via open histogram. Total cells were used for analysis.
Predicted band size: 22kDa. Positive control: Hela cell lysate . Recommended dilution: 1/500-2000
Western blot analysis of BCL-2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of HeLa cell line, using BCL2 antibody.
Immunohistochemistry staining of Paraffin Tonsil tissue by BCL2 antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
IHC staining of paraffin-embedded tonsil tissue with BCL2 antibody TA327607 at 1:100 dilution.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Bcl-2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody recognizes both the a and ß Bcl-2 isoforms.
Immunofluorescent microscope analysis of Hela cells using anti-Bcl-2 polyclonal antibody, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.
Western blot analysis of extracts of various cell lines using BCL2 antibody.
Immunohistochemistry of paraffin-embedded Breast cancer using BCL2 Antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using BCL2 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded mouse lung using BCL2 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BCL2 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded rat lung using BCL2 antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of A549 cell using BCL2 antibody. Blue: DAPI for nuclear staining.
Western blot analysis on MCF7 cell lysate using BCL-2 Antibody
Western blot analysis of BCL2 expression in Hela (A) whole cell lysates.
Immunohistochemical analysis of BCL2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of BCL2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Phospho-BCL2-T56 antibody.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma, using Phospho-BCL2-S70 antibody.
Western blot analysis of extracts from 293 cells. Jurkat cells and NIH/3T3 cells all treated with Paclitaxel, using BCL2 (Phospho-Ser70) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of BCL-2 phosphorylation expression in nocodazole treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of BCL-2 phosphorylation expression in LPS treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM10050SU-N | Bcl-2 antibody |
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 |
Mouse | IgG1 | 124 | Supernatant | Hu | P, WB |
1 ml /
€450.00
|
| OriGene Technologies GmbH | |||||||
| AM32275SU-N | Bcl-2 antibody |
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 |
Rabbit | IgG | E17 | Supernatant | Hu | C, P |
1 ml /
€820.00
|
| OriGene Technologies GmbH | |||||||