Bcl-3 antibody
Principal name
Bcl-3 antibody
Alternative names for Bcl-3 antibody
BCL3, BCL4, D19S37, B-cell lymphoma 3-encoded protein
Ncbi ID
NP_005169.2, NP_291079.2, 10090, 9606, NP_005169, NM_005178, NM_033601
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of Bcl-3 antibody
- Kit Component:
- - KN302109G1, Bcl3 gRNA vector 1 in pCas-Guide vector
- KN302109G2, Bcl3 gRNA vector 2 in pCas-Guide vector
- KN302109D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of Bcl-3 in Jurkat cell lysate using antibody AP00151PU-N
Immunofluorescence of purified MaxPab antibody to BCL3 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of BCL3 expression in transfected 293T cell line (H00000602-T01) by BCL3 MaxPab polyclonal antibody.Lane 1: BCL3 transfected lysate(49.06 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BCL3 expression in transfected 293T cell line (H00000602-T02) by BCL3 MaxPab polyclonal antibody.Lane 1: BCL3 transfected lysate(46.80 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of BCL3 expression in Jurkat cell lysate.
Immunoprecipitation of BCL3 transfected lysate using anti-BCL3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BCL3 MaxPab mouse polyclonal antibody (B01) (H00000602-B01).
Western Blot Analysis of AM31856PU-N bcl-3 antibody: Lysate from Mouse Spleen and Thymus (Thymus shown above) cells probed with AM31856PU-Nat 1.0 µg/ml. AP conjugated Goat Anti-Hamster IgG as secondary antibody.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of BCL3 Antibody (Center) (Cat#AP50358PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of BCL3 Antibody (Center) (Cat. #AP50358PU-N) in mouse liver tissue lysates (35µg/lane). BCL3 (arrow) was detected using the purified Pab.
BCL3 Antibody (Center) (Cat. #AP50358PU-N) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of Jurkat cells (Lane 1: 20ug and Lane 2: 40ug cell lysate) with anti-BCL3 antibody.
Western blot analysis of BCL3 in 293 cell lysate with BCL3 antibody at 1 ug/mL.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, hela cells, Primary antibody: (BCL3 Antibody) at dilution 1/333, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (BCL3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (BCL3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP50358PU-N | Bcl-3 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | F, ICC/IF, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA310105 | Bcl-3 antibody |
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| Rabbit anti-Bcl3 polyclonal antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€195.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319619 | Bcl-3 antibody |
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| Rabbit Polyclonal BCL3 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350797 | Bcl-3 antibody |
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| Rabbit Polyclonal Anti-BCL3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM31856BT-L | Bcl-3 antibody |
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Hamster | IgG1 | Ham150-3.5 | Biotin | Hu, Ms | E |
0.3 mg /
€500.00
|
| OriGene Technologies GmbH | |||||||
| AM31856BT-N | Bcl-3 antibody |
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|
Hamster | IgG1 | Ham150-3.5 | Biotin | Hu, Ms | E |
0.1 mg /
€240.00
|
| OriGene Technologies GmbH | |||||||
| AM31856FC-L | Bcl-3 antibody |
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Hamster | IgG1 | Ham150-3.5 | FITC | Hu, Ms | F |
0.3 mg /
€500.00
|
| OriGene Technologies GmbH | |||||||
| AM31856FC-N | Bcl-3 antibody |
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|
Hamster | IgG1 | Ham150-3.5 | FITC | Hu, Ms | F |
0.1 mg /
€260.00
|
| OriGene Technologies GmbH | |||||||
| AM31856PU-N | Bcl-3 antibody |
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 |
Hamster | IgG1 | Ham150-3.5 | Purified | Hu, Ms | E, F, WB |
0.25 mg /
€360.00
|
| OriGene Technologies GmbH | |||||||
| AM31856RP-L | Bcl-3 antibody |
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Hamster | IgG1 | Ham150-3.5 | PE | Hu, Ms | F |
0.3 mg /
€580.00
|
| OriGene Technologies GmbH | |||||||
| AM31856RP-N | Bcl-3 antibody |
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Hamster | IgG1 | Ham150-3.5 | PE | Hu, Ms | F |
50 µg /
€210.00
|
| OriGene Technologies GmbH | |||||||
| AP00151PU-N | Bcl-3 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | IP, P, WB |
0.1 mg /
€395.00
|
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| OriGene Technologies GmbH | |||||||
| MO25032-100 | Bcl-3 antibody |
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Hamster | IgG1 | HAM150-3.5 | Ascites | Ms | C, WB |
0.1 ml /
€370.00
|
| Neuromics Antibodies | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309116 | Bcl-3 |
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| Recombinant protein of human B-cell CLL/lymphoma 3 (BCL3) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417469 | BCL3 overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR225844L2V | Lenti ORF particles, Bcl3 (GFP-tagged) - Mouse B-cell leukemia/lymphoma 3 (Bcl3), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||