BDH2 antibody
Principal name
BDH2 antibody
Alternative names for BDH2 antibody
DHRS6, Oxidoreductase UCPA, 3-hydroxybutyrate dehydrogenase type 2, R-beta-hydroxybutyrate dehydrogenase, Dehydrogenase/reductase SDR family member 6
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of BDH2 antibody
BDH2, also known as DHRS6, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of BDH2 make it suitable to convert high levels of circulating 3-hydroxybutyrate into acetoacetate.
General readings
1. Buhrke T, Lengler I, Lampen A. Analysis of proteomic changes induced upon cellular differentiation of the human intestinal cell line Caco-2. Dev Growth Differ. 2011 Apr;53(3):411-26
PMID: 21492153
BDH2 polyclonal antibody ( Cat # PAB7523 ) ( 0.3 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
DHRS6 MaxPab polyclonal antibody. Western Blot analysis of DHRS6 expression in human kidney.
Western Blot analysis of BDH2 expression in transfected 293T cell line (H00056898-T01) by BDH2 MaxPab polyclonal antibody.Lane 1: DHRS6 transfected lysate(26.95 KDa).Lane 2: Non-transfected lysate.
BDH2 MaxPab polyclonal antibody. Western Blot analysis of BDH2 expression in HepG2.
DHRS6 MaxPab polyclonal antibody. Western Blot analysis of DHRS6 expression in human kidney.
Western Blot analysis of BDH2 expression in transfected 293T cell line (H00056898-T01) by BDH2 MaxPab polyclonal antibody.Lane 1: DHRS6 transfected lysate(26.95 KDa).Lane 2: Non-transfected lysate.
BDH2 MaxPab polyclonal antibody. Western Blot analysis of BDH2 expression in HepG2.
Human Jurkat; WB Suggested Anti-BDH2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; BDH2 antibody - middle region (ARP57360_P050) in Human Jurkat cells using Western Blot
Recombinant human BDH2, 1-245aa, His-tagged
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501291, Dilution 1:50)
Anti-BDH2 mouse monoclonal antibody (TA501291) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BDH2(RC210586).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (RC210586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2.
Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-BDH2 monoclonal antibody.(1:200)
HEK293T cells transfected with either RC210586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BDH2 antibody(TA501291), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-BDH2 antibody(TA501291),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-BDH2 antibody(TA501291),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501292, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501292, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501292, Dilution 1:50)
Anti-BDH2 mouse monoclonal antibody (TA501292) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BDH2(RC210586).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (RC210586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2.
HEK293T cells transfected with either RC210586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BDH2 antibody(TA501292), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501293, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501293, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501293, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501293, Dilution 1:50)
Anti-BDH2 mouse monoclonal antibody (TA501293) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BDH2(RC210586).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (RC210586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2.
HEK293T cells transfected with either RC210586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BDH2 antibody(TA501293), and then analyzed by flow cytometry.
TA302431 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing BDH2 (RC210586) and probed with TA302431 (mock transfection in first lane)
WB Suggested Anti-BDH2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501291 | BDH2 antibody |
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| anti-BDH2 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4) | |||||||
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Mouse | IgG2a | OTI4G4 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
|
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| TA501292 | BDH2 antibody |
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| anti-BDH2 mouse monoclonal antibody, clone OTI3G10 (formerly 3G10) | |||||||
 |
Mouse | IgG1 | OTI3G10 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA501293 | BDH2 antibody |
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| anti-BDH2 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1) | |||||||
 |
Mouse | IgG1 | OTI2G1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA302431 | BDH2 (60-71) antibody |
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| Goat Polyclonal Antibody against BDH2 / DHRS6 (aa 60 to 71) | |||||||
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Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310586 | BDH2 |
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| Recombinant protein of human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720509 | BDH2 |
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| Recombinant protein of human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203075L2V | Lenti ORF particles, Bdh2 (GFP-tagged) - Mouse 3-hydroxybutyrate dehydrogenase, type 2 (Bdh2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203075L1V | Lenti ORF particles, Bdh2 (Myc-DDK-tagged) - Mouse 3-hydroxybutyrate dehydrogenase, type 2 (Bdh2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||