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BIK antibody

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Principal name

BIK antibody

Alternative names for BIK antibody

Bcl-2-interacting killer, NBK, BP4, BIP1, Apoptosis inducer NBK

SwissProt ID

Q13323 (Human)

Gene ID

638 (BIK)

Ncbi ID

9606, NP_001188.1, NP_001188, CAG30276, NM_001197

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP)

Background of BIK antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b(5) resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.

H00000638-M01 - BIK
Immunoperoxidase of monoclonal antibody to BIK on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
H00000638-M01 - BIK
Detection limit for recombinant GST tagged BIK is approximately 0.03ng/ml as a capture antibody.
AP06031PU-N - BIK
Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using BIK antibody (Cat.-No. AP06031PU-N).
AP06031PU-N - BIK
Western blot (WB) analysis of BIK antibody (Cat.-No.: AP06031PU-N) in extracts from A549 cell treated with DMSO 0.1% 10'.
AP07668PU-N - BIK
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate.
AP05587PU-N - BIK
Immunocytochemical staining of Jurkat cells with Rabbit anti Human BIK (AP05587PU-N)
AP05587PU-N - BIK
Western blot analysis of whole cell lysate from Jurkat, human T-cell leukemia cells probed with Rabbit anti Human BIK (AP05587PU-N) at 1(A) and 2(B) µg/ml
PAB0239 - BIK
Formalin-fixed, paraffin-embedded tissue section of human kidney stained for BIK expression. Using BIK polyclonal antibody ( Cat # PAB0239 ) at 1 : 2000. A high level of BIK signal was seen in the distal collecting tubules, the glomeruli are primarily negative. Hematoxylin-eosin counterstain.
PAB2449 - BIK
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the BIK polyclonal antibody ( Cat # PAB2449 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB2449 - BIK
The BIK polyclonal antibody ( Cat # PAB2449 ) is used in Western blot to detect BIK in mouse brain tissue lysate.
AP11315PU-N - BIK
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11315PU-N - BIK
Western blot analysis using anti-Bik BH3 domain Pab (Cat. #AP11315PU-N) to detect Bik BH3 in mouse brain tissue lysate.
AP12573PU-N - BIK
AP12573PU-N BIK antibody staining of Formalin-Fixed and Paraffin-Embedded Human cancer tissue. The primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP12573PU-N - BIK
Western blot analysis of anti-Bik Pab (Cat.#AP12573PU-N) in mouse liver tissue lysate (35ug/lane). Bik(arrow) was detected using the purified Pab (1:60 dilution).
PAB12962 - BIK
Immunocytochemistry of BIK in Jurkat cells with BIK polyclonal antibody ( Cat # PAB12962 ) at 1 µg/ml.
PAB12962 - BIK
Western blot analysis of BIK in Jurkat cell lysate with BIK polyclonal antibody ( Cat # PAB12962 ) at ( A ) 1 and ( B ) 2 µg/ml.
A0053-1 - BIK
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BIK (Phospho-Thr33) Antibody. The picture on the right is treated with the synthesized peptide.
A0053-1 - BIK
Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) Antibody. The lane on the right is treated with the synthesized peptide.
B0053-1 - BIK
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Ab-33) Antibody. The picture on the right is treated with the synthesized peptide.
B0053-1 - BIK
Immunofluorescence analysis of HuvEc cells, using BIK (Ab-33) Antibody. The picture on the right is treated with the synthesized peptide.
B0053-1 - BIK
Western blot analysis of extracts from HuvEc/COLO205, using BIK (Ab-33) Antibody. The lane on the right is treated with the synthesized peptide.
AM31725PU-N - BIK
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31725PU-N - BIK
Detection limit for recombinant GST tagged BIK is approximately 0.03ng/ml as a capture antibody.
CPA3146-100ul - BIK
Immunohistochemical analysis of BIK (pT33) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3146-100ul - BIK
Western blot analysis of BIK (pT33) expression in Jarkat (A); K562 (B) whole cell lysates.
TP760492 - BIK
TA306325 - BIK
Western blot analysis of BIK in Jurkat cell lysate with BIK antibody at (A) 1 and (B) 2 ug/mL.
TA306325 - BIK
Immunocytochemistry of BIK in Jurkat cells with BIK antibody at 1 ug/mL.
TA306325 - BIK
Immunofluorescence of Bik in Jurkat cells with Bik antibody at 10 ug/mL.
TA325271 - BIK
Western blot analysis of BIK expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA325272 - BIK
Western blot analysis of BIK phosphorylation expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA313505 - BIK
Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody (#TA313505, Line 1 and 2)The lane on the right is treated with the synthesized peptide.
TA313505 - BIK
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody (#TA313505)The picture on the right is treated with the synthesized peptide.

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BIK antibody
  Rabbit Polyclonal Bik Antibody  
Western blot analysis of BIK in Jurkat cell lysate with BIK antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

BIK (N-term) antibody
Immunocytochemical staining of Jurkat cells with Rabbit anti Human BIK (AP05587PU-N) Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.1 mg / €410.00
  OriGene Technologies GmbH
+1 additional image

BIK antibody
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

BIK (BH3 Domain) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

4 Item(s)

per page

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Product Type
  1. Primary Antibody (4)
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  1. Polyclonal (4)
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  2. N-term (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  3. Paraffin Sections (P) (2)
  4. Enzyme Immunoassay (E) (1)
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  2. Purified (2)
Host
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Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Immunofluorescence (IF) (1)
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