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Biotin antibody

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Principal name

Biotin antibody

Alternative names for Biotin antibody

Vitamin B7, Vitamin H

Available reactivities

Hu (Human)

Available hosts

Goat, Mouse, Sheep, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunodiffusion (ID), Immunoprecipitation (IP), Dot blot (Dot)

Background of Biotin antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

General readings

General / background references:Titus JA et al. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Methods 50:193-204 (1982). PubMed PMID: 6806389

RA104GAL - Biotin
Dot Blot of Biotin Conjugated Beta Galactosidase Antigen: Lane 1: Biotin Conjugated Beta Galactosidase. Lane 2: Beta Galactosidasse Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1/40,000 for 30 min at RT Block: 1% BSA-TTBS 30 min at RT
RA104GLU - Biotin
Dot Blot of Biotin Glucose Oxidase Conjugated Antigen:Lane 1: Biotin Conjugated Glucose Oxidase. Lane 2: Glucose Oxidase. Load: 3-fold serial dilution starting at 200 ng Secondary antibody: HRP Streptavidin at 1/40,000 for 30 min at RT Block: 1% BSA-TTBS 30 min at RT
GTX77579 - Biotin
GTX77581 - Biotin
AM20209PU-N - Biotin
Antibody Sensitivity: Biotinylated Molecular Weight Marker (Biorad) was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with Biotin antibody AM20209PU-N (Clone 2H2) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: 200nl Lane 2: 100nlLane 3: 50nlLane 4: 20nlLane 5: 10nlLane 6: 5nlLane 7: 2nl

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Biotin antibody
Biotin Mouse IgG1 B25 (BGN/09/6225) Purified E
0.2 mg / €460.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Mouse IgG1 B-E24 Azide Free Hu F, P
0.2 mg / €350.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Mouse IgG3 BIO-8 Purified C
1 ml / €430.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Goat Serum Dot, E, ID, IP, WB
2 ml / €340.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG Serum Dot, E, IP, WB
2 ml / €510.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Goat IgG DTAF F, ICC/IF
20 mg / €3,815.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG FITC C, F, ICC/IF
20 mg / €3,815.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG Texas Red E, F
20 mg / €3,815.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Goat IgG PE F
1 ml / €640.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Goat IgG TRITC F
1 mg / €630.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG Rhodamine F, ICC/IF
0.1 ml / €480.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG AP C, Dot, E, WB
0.1 mg / €480.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG FITC F, ICC/IF
0.1 ml / €480.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG HRP C, E, WB
0.1 mg / €480.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG Aff - Purified E, ID, IP, WB
0.1 mg / €355.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG PE F, ICC/IF
0.1 ml / €480.00
  OriGene Technologies GmbH

Biotin antibody
Biotin Rabbit IgG Texas Red F, ICC/IF
0.1 ml / €480.00
  OriGene Technologies GmbH

Biotin (F(ab')2 fragment) antibody
Biotin Goat IgG AP C, Dot, E, WB
1 mg / €615.00
  OriGene Technologies GmbH

Biotin (F(ab')2 fragment) antibody
Biotin Goat IgG FITC F, ICC/IF
1 mg / €615.00
  OriGene Technologies GmbH

Biotin (F(ab')2 fragment) antibody
Biotin Goat IgG HRP C, Dot, E, WB
1 mg / €615.00
  OriGene Technologies GmbH

Items 1 to 20 of 33 total

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33 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (29)
  2. Protein | Growth Factor (4)
Clonality
  1. Polyclonal (26)
  2. Monoclonal (3)
Target
  1. Biotin (33)
Property
  1. F(ab')2 fragment (7)
Reactivity
  1. Human (Hu) (1)
Applications
  1. Flow cytometry (F) (16)
  2. Enzyme Immunoassay (E) (15)
  3. Western blot / Immunoblot (WB) (13)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (11)
  5. Frozen sections (C) (10)
  6. Dot blot (Dot) (5)
  7. Immunoprecipitation (IP) (5)
  8. Paraffin Sections (P) (5)
  9. Immunodiffusion (ID) (3)
Presentation
  1. AP (4)
  2. FITC (4)
  3. HRP (4)
  4. Texas Red (4)
  5. Aff - Purified (3)
  6. PE (3)
  7. Purified (2)
  8. Serum (2)
  9. TRITC (2)
  10. Azide Free (1)
  11. DTAF (1)
  12. Glucose Oxidase (1)
  13. Rhodamine (1)
Host
  1. Goat (16)
  2. Rabbit (10)
  3. Mouse (3)
Images
  1. 1 image (2)
Images: Applications
  1. Dot blot (Dot) (2)
Manufacturer
  1. OriGene Technologies (33)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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