Biotinidase antibody
Principal name
Biotinidase antibody
Alternative names for Biotinidase antibody
BTD, Biotinase
Ncbi ID
9606, NP_000051.1, NP_000051, NP_079571.1, NM_025295, NM_000060, NM_001012047
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP)
Background of Biotinidase antibody
- Kit Component:
- - KN204153G1, BTD gRNA vector 1 in pCas-Guide vector
- KN204153G2, BTD gRNA vector 2 in pCas-Guide vector
- KN204153D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of BTD expression in transfected 293T cell line (H00000686-T01) by BTD MaxPab polyclonal antibody.Lane 1: BTD transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BTD expression in transfected 293T cell line (H00000686-T01) by BTD MaxPab polyclonal antibody.Lane 1: BTD transfected lysate(61.10 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BTD expression in transfected 293T cell line by BTD monoclonal antibody (M01), clone 3B10-2B3.Lane 1: BTD transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of BTD transfected lysate using anti-BTD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BTD MaxPab rabbit polyclonal antibody.
Staining of Human Liver lysate using Biotinidase antibody Cat.-No AP32132PU-N at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32132PU-N | Biotinidase (372-385) antibody |
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 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304153 | Biotinidase |
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| Recombinant protein of human biotinidase (BTD) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208483L2V | Lenti ORF particles, Btd (GFP-tagged) - Mouse biotinidase (Btd), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,230.00
|
|
| OriGene Technologies, Inc. | ||
| MR208483L1V | Lenti ORF particles, Btd (Myc-DDK-tagged) - Mouse biotinidase (Btd), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201713L2V | Lenti ORF particles, Btd (GFP-tagged ORF) - Rat biotinidase (Btd), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
| RR201713L1V | Lenti ORF particles, Btd (Myc-DDK-tagged ORF) - Rat biotinidase (Btd), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||
