BNIP1 antibody
Principal name
BNIP1 antibody
Alternative names for BNIP1 antibody
NIP1, SEC20L, TRG8, Vesicle transport protein SEC20, BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, Transformation-related gene 8 protein
Ncbi ID
NP_001196.2, 9606, NP_001196, XP_002044748.1, NM_001205, NP_742161.4, NM_172149, NM_080897
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of BNIP1 antibody
- Kit Component:
- - KN302212G1, Bnip1 gRNA vector 1 in pCas-Guide vector
- KN302212G2, Bnip1 gRNA vector 2 in pCas-Guide vector
- KN302212D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Nakajima K, Hirose H, Taniguchi M, Kurashina H, Arasaki K, Nagahama M, Tani K, Yamamoto A, Tagaya M.
Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion.
EMBO J. 2004 Aug 18;23(16):3216-26. Epub 2004 Jul 22.
PMID: 15272311
BNIP1 MaxPab polyclonal antibody. Western Blot analysis of BNIP1 expression in human pancreas.
Western Blot analysis of BNIP1 expression in transfected 293T cell line (H00000662-T01) by BNIP1 MaxPab polyclonal antibody.Lane1:BNIP1 transfected lysate(25.19 KDa).Lane 2:Non-transfected lysate.
BNIP1 polyclonal antibody ( Cat # PAB7347 ) ( 0.5 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Detection limit for recombinant GST tagged BNIP1 is approximately 3ng/ml as a capture antibody.
BNIP1 MaxPab polyclonal antibody. Western Blot analysis of BNIP1 expression in human pancreas.
Western Blot analysis of BNIP1 expression in transfected 293T cell line (H00000662-T01) by BNIP1 MaxPab polyclonal antibody.Lane1:BNIP1 transfected lysate(25.19 KDa).Lane 2:Non-transfected lysate.
Human Liver; WB Suggested Anti-BNIP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; BNIP1 antibody - N-terminal region (ARP59522_P050) in Human Liver cells using Western Blot
Human Liver; WB Suggested Anti-BNIP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; BNIP1 antibody - C-terminal region (ARP59521_P050) in Human Liver cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802188)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802188)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802188)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802188)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802188)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802188)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802217)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802217)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802217)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BNIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802217)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1 (RC203147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BNIP1.
TA302435 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 26kDa. Positive control: A431 and A172 cell, human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1:A431 cell lysate Lane2: A172 cell lysate Lane3: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds)
Predicted cell location: Nucleus. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using BNIP1 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 26kDa. Positive control: Jurkat and A172 cell, human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: A172 cell lysate Lane3: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/275 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using BNIP1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR215918L2V | Lenti ORF particles, Bnip1 (GFP-tagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR215918L1V | Lenti ORF particles, Bnip1 (Myc-DDK-tagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
