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BPI antibody

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Principal name

BPI antibody

Alternative names for BPI antibody

CAP57, Bactericidal permeability-increasing protein

SwissProt ID

P17213 (Human)

Gene ID

671 (BPI)

Ncbi ID

NP_001716, 9606, NP_001716.2, NM_001725

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Functional assay (FN), Immunoprecipitation (IP)

Background of BPI antibody

The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airways disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin.

AP50386PU-N - BPI
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP50386PU-N - BPI
Western blot analysis of BPI Antibody (Center) (Cat. #AP50386PU-N) in HL-60 cell line lysates (35µg/lane). BPI (arrow) was detected using the purified Pab.
AP50386PU-N - BPI
BPI Antibody (Center) (Cat. #AP50386PU-N) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TP308067 - BPI
TA332620 - BPI
Western blot analysis of extracts of various cell lines, using BPI antibody.
TA332620 - BPI
Immunohistochemical of paraffin-embedded mouse lung using BPI antibody at dilution of 1:200 (400x lens).
TA332620 - BPI
Immunohistochemical of paraffin-embedded mouse cancer using BPI antibody at dilution of 1:200 (400x lens).
TA332620 - BPI
Immunofluorescence analysis of A549 cell using BPI antibody. Blue: DAPI for nuclear staining.
TA348932 - BPI
TA348932 (0.3 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA349719 - BPI
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (BPI Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA349719 - BPI
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (BPI Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
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  2. Highly Validated (1)

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BPI antibody
  Rabbit anti-BPI Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using BPI antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

BPI antibody
  Goat Polyclonal Anti-BPI Antibody  
TA348932 (0.3 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

BPI antibody
  Rabbit Polyclonal Anti-BPI Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (BPI Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

BPI antibody
BPI Mouse IgG1 GE 4.90 Purified Hu E, FN
0.1 mg / €490.00
  OriGene Technologies GmbH

BPI antibody
BPI Mouse IgG1 4H5 Purified Hu E
0.1 mg / €450.00
  OriGene Technologies GmbH

BPI antibody
BPI Mouse IgG1 3F9 Purified Hu E
0.1 mg / €450.00
  OriGene Technologies GmbH

BPI antibody
BPI Rabbit IgG Purified E, IP
0.1 mg / €450.00
  OriGene Technologies GmbH

BPI (Center) antibody
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BPI
  Recombinant protein of human bactericidal/permeability-increasing protein (BPI)  
BPI Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

BPI
  Purified recombinant protein of Human bactericidal/permeability-increasing protein (BPI)  
BPI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

BPI overexpression lysate
BPI overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

11 Item(s)

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Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (8)
  3. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (3)
Target
  1. BPI (10)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (1)
Applications
  1. Enzyme Immunoassay (E) (5)
  2. Paraffin Sections (P) (2)
  3. Western blot / Immunoblot (WB) (2)
  4. Flow cytometry (F) (1)
  5. Functional assay (FN) (1)
  6. Immunoprecipitation (IP) (1)
Presentation
  1. Purified (8)
Host
  1. Rabbit (4)
  2. Mouse (3)
  3. Goat (1)
Images
  1. 1 image (5)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. IHC (2)
  3. Flow cytometry (F) (1)
  4. Immunofluorescence (IF) (1)
  5. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (11)
Service
  • OriGene Technologies GmbH
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  • Germany
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