BRD3 antibody
Principal name
BRD3 antibody
Alternative names for BRD3 antibody
KIAA0043, RING3L, RING3-like protein, Bromodomain-containing protein 3
Ncbi ID
9606, AAH32124, NP_031397, NP_031397.1, NP_001107045.1, NM_001113573, NM_023336, NM_001113574, NM_007371
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)
Background of BRD3 antibody
- Kit Component:
- - KN302253G1, Brd3 gRNA vector 1 in pCas-Guide vector
- KN302253G2, Brd3 gRNA vector 2 in pCas-Guide vector
- KN302253D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ishii,H., (2005) DNA Cell Biol. 24 (7), 432-437
Immunoperoxidase of monoclonal antibody to BRD3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
BRD3 monoclonal antibody (M01), clone 6E7 Western Blot analysis of BRD3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged BRD3 is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to BRD3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
BRD3 monoclonal antibody (M02), clone 7G6 Western Blot analysis of BRD3 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged BRD3 is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to BRD3 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
BRD3 monoclonal antibody (M03), clone 6C10. Western Blot analysis of BRD3 expression in human kidney.
BRD3 monoclonal antibody (M03), clone 6C10 Western Blot analysis of BRD3 expression in Hela NE ( Cat # L013V3 ).
Immunoperoxidase of monoclonal antibody to BRD3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
BRD3 monoclonal antibody (M04), clone 6F3 Western Blot analysis of BRD3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged BRD3 is approximately 0.1ng/ml as a capture antibody.
BRD3 polyclonal antibody (A01), Lot # 060707JCS1 Western Blot analysis of BRD3 expression in U-2 OS ( Cat # L022V1 ).
Human Jurkat; WB Suggested Anti-BRD3 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. BRD3 is supported by BioGPS gene expression data to be expressed in Jurkat.; BRD3 antibody - N-terminal region (ARP38939_T100) in Human Jurkat cells using Western Blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BRD3 polyclonal antibody ( Cat # PAB3246 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The BRD3 polyclonal antibody ( Cat # PAB3246 ) is used in Western blot to detect BRD3 in mouse brain tissue lysate ( Lane 1 ) and Sacs-2 cell lysate.
BRD3 monoclonal antibody (M03), clone 6C10. Western Blot analysis of BRD3 expression in human kidney.
BRD3 monoclonal antibody (M03), clone 6C10 Western Blot analysis of BRD3 expression in Hela NE ( Cat # L013V3 ).
Immunofluorescence of purified MaxPab antibody to BRD3 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of BRD3 expression in transfected 293T cell line (H00008019-T01) by BRD3 MaxPab polyclonal antibody.Lane 1: BRD3 transfected lysate(61.16 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BRD3 expression in transfected 293T cell line (H00008019-T01) by BRD3 MaxPab polyclonal antibody.Lane 1: BRD3 transfected lysate(60.90 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-BRD3 Pab is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).
Human brain; WB Suggested Anti-BRD3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; BRD3 antibody - middle region (ARP34431_P050) in Human brain cells using Western Blot
Hum. Adult Placenta; Host: Rabbit . Target Name: BRD3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; BRD3 antibody - middle region (ARP34431_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: BRD3 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; BRD3 antibody - middle region (ARP34431_P050) in Hum. Fetal Lung cells using Western Blot
Hum. Adult Placenta; BRD3 antibody - middle region (ARP34431_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Lung; BRD3 antibody - middle region (ARP34431_P050) in Hum. Fetal Lung cells using Western Blot
Human Thymus; WB Suggested Anti-BRD3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Thymus; BRD3 antibody - middle region (ARP38940_P050) in Human Thymus cells using Western Blot
Immunohistochemical analysis of BRD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of BRD3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of BRD3 expression in Saos2 (A); HepG2 (B) whole cell lysates.
Western blot analysis of extracts from A549 cells, using BRD3 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2), using BRD3 Antibody. The lane on the left is treated with synthesized peptide.
WB Suggested Anti-BRD3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain
Host: Rabbit; Target Name: BRD3; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: BRD3; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
Hum. Fetal Lung
Hum. Adult Placenta
WB Suggested Anti-BRD3 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate; BRD3 is supported by BioGPS gene expression data to be expressed in Jurkat
WB Suggested Anti-BRD3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314713 | BRD3 antibody |
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| Rabbit polyclonal anti-BRD3 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP14979PU-N | BRD3 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210310L2V | Lenti ORF particles, Brd3 (GFP-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 2, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,500.00
|
|
| OriGene Technologies, Inc. | ||
| MR210310L1V | Lenti ORF particles, Brd3 (Myc-DDK-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,350.00
|
|
| OriGene Technologies, Inc. | ||