BRD4 antibody
Principal name
BRD4 antibody
Alternative names for BRD4 antibody
HUNK1, Bromodomain-containing protein 4
Ncbi ID
9606, NP_055114, NM_014299, NP_055114.1, NP_065254.3, NM_198094, NM_020508, NM_058243
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of BRD4 antibody
- Kit Component:
- - KN302254G1, Brd4 gRNA vector 1 in pCas-Guide vector
- KN302254G2, Brd4 gRNA vector 2 in pCas-Guide vector
- KN302254D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Schweiger,M.R., (2006) J. Virol. 80 (9), 4276-4285
Western blot analysis of Brd4 in HeLa lysate in the 1 ) absence and 2 ) presence of immunizing peptide. Using BRD4 polyclonal antibody ( Cat # PAB0292 ) at 0.5 µg/ml.
HeLa; . Sample Type :. HeLa. . Primary Antibody Dilution:. 4 ug/ml . Secondary Antibody :. Anti-rabbit Alexa 546 . Secondary Antibody Dilution:. 2 ug/ml . Gene Name :. BRD4; BRD4 antibody - C-terminal region (ARP39076_P050) in HeLa cells using Immunofluorescence
Human Thymus; WB Suggested Anti-BRD4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Thymus; BRD4 antibody - C-terminal region (ARP39076_P050) in Human Thymus cells using Western Blot
HCT116; Chromatin Immunoprecipitation (ChIP) Using BRD4 antibody - C-terminal region (ARP39076_P050) and HCT116 Cells; BRD4 antibody - C-terminal region (ARP39076_P050) in HCT116 cells using Chromatin Immunoprecitation
Western Blot analysis of BRD4 expression in transfected 293T cell line (H00023476-T02) by BRD4 MaxPab polyclonal antibody.Lane 1: BRD4 transfected lysate(88.30 KDa).Lane 2: Non-transfected lysate.
Western Blot: BRD4 Antibody [TA336367] - analysis of Brd4 in human HeLa lysate in the 1) absence and 2) presence of immunizing peptide and C) mouse NIH 3T3 lysate using Brd4 antibody at 3 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect EC
Host: Rabbit; Target Name: Brd4; Sample Tissue: Rat Small Intestine lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-BRD4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Thymus
Chromatin Immunoprecipitation (ChIP) Using BRD4 antibody - C-terminal region and HCT116 Cells
Sample Type: HeLa; Primary Antibody Dilution: 4 ug/ml; Secondary Antibody: Anti-rabbit Alexa 546; Secondary Antibody Dilution: 2 ug/ml; Gene Name: BRD4
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336367 | BRD4 antibody |
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| Rabbit Polyclonal BRD4 Antibody | |||||||
![Western Blot: BRD4 Antibody [TA336367] - analysis of Brd4 in human HeLa lysate in the 1) absence and 2) presence of immunizing peptide and C) mouse NIH 3T3 lysate using Brd4 antibody at 3 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect EC](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-56487-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Prim, Rt | WB |
0.1 mg /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| TA350939 | BRD4 antibody |
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| Rabbit Polyclonal Anti-BRD4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN216879 | BRD4 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN216879BN | BRD4 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN216879LP | BRD4 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN216879RB | BRD4 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY415375 | BRD4 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC218845L2V | Lenti ORF particles, BRD4 (mGFP-tagged) - Human bromodomain containing 4 (BRD4), transcript variant long, 200 uL, >10^7 TU/mL |
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|
200 µl /
€2,680.00
|
|
| OriGene Technologies, Inc. | ||
| RC218845L1V | Lenti ORF particles, BRD4 (Myc-DDK tagged) - Human bromodomain containing 4 (BRD4), transcript variant long , 200 uL, >10^7 TU/mL |
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|
200 µl /
€2,420.00
|
|
| OriGene Technologies, Inc. | ||