BTG2 antibody
Principal name
BTG2 antibody
Alternative names for BTG2 antibody
PC3, BTG family member 2, NGF-inducible anti-proliferative protein PC3
Ncbi ID
9606, NP_006754, NP_006754.1, NM_006763, NP_031596.1, NM_007570
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of BTG2 antibody
- Kit Component:
- - KN302307G1, Btg2 gRNA vector 1 in pCas-Guide vector
- KN302307G2, Btg2 gRNA vector 2 in pCas-Guide vector
- KN302307D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Morel,A.P., et al., (2003) J. Cell. Sci. 116 (Pt 14), 2929-2936
Western Blot analysis of BTG2 expression in transfected 293T cell line by BTG2 monoclonal antibody (M01), clone 1A5.Lane 1: BTG2 transfected lysate(17.4 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BTG2 expression in transfected 293T cell line by BTG2 monoclonal antibody (M02), clone 1B8.Lane 1: BTG2 transfected lysate(17.4 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BTF3L4 expression in transfected 293T cell line (H00091408-T01) by BTF3L4 MaxPab polyclonal antibody.Lane 1: BTF3L4 transfected lysate(17.38 KDa).Lane 2: Non-transfected lysate.
Human Lung; WB Suggested Anti-BTG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Lung; BTG2 antibody - middle region (ARP33561_P050) in Human Lung cells using Western Blot
Human ACHN; WB Suggested Anti-BTG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: ACHN cell lysate. BTG2 is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells.; BTG2 antibody - N-terminal region (ARP33560_P050) in Human ACHN cells using Western Blot
BTG2 Antibody (N-term) (Cat. #AP50404PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTG2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
BTG2 Antibody (N-term) (Cat. #AP50404PU-N) western blot analysis in K562 cell line lysates (35µg/lane).This demonstrates the BTG2 antibody detected the BTG2 protein (arrow).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808149)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808149)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:500)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:500)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808197)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808197)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808197)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BTG2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808197)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTG2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808197)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2.(1:2000)
Western blot analysis of Hep-2 lysates with anti-BTG2 antibody (1:250). This antibody identified a band of ~17kDa. The upper bands were non-specific for loading controls.
Predicted band size: 17 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
Predicted band size: 17 kDa . Positive control: PC3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using BTG2 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-BTG2 Antibody Titration: 0.2-1 ug/ml; Positive Control: ACHN cell lysateBTG2 is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells
WB Suggested Anti-BTG2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Lung
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808197 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI4C10 (formerly 4C10) | |||||||
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Mouse | IgG1 | OTI4C10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| TA310107 | BTG2 antibody |
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| Rabbit anti-BTG2 polyclonal antibody | |||||||
 |
Rabbit | Purified | Hu | WB |
0.1 ml /
€220.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA321783 | BTG2 antibody |
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| Anti-BTG2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321784 | BTG2 antibody |
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| Anti-BTG2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA808149 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3) | |||||||
 |
Mouse | IgG1 | OTI1G3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA808150 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI2F3 (formerly 2F3) | |||||||
 |
Mouse | IgG1 | OTI2F3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808151 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5) | |||||||
 |
Mouse | IgG1 | OTI2G5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808152 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI7A10 (formerly 7A10) | |||||||
 |
Mouse | IgG2a | OTI7A10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808153 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3) | |||||||
 |
Mouse | IgG1 | OTI9D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808155 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI5C7 (formerly 5C7) | |||||||
 |
Mouse | IgG2b | OTI5C7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808268 | BTG2 antibody |
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| BTG2 mouse monoclonal antibody, clone OTI4H8 (formerly 4H8) | |||||||
 |
Mouse | IgG1 | OTI4H8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP50404PU-N | BTG2 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760250 | BTG2 (full length, N-term HIS tag) |
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| Recombinant protein of human BTG family, member 2 (BTG2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402022 | BTG2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |