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Bu-1b antibody

Principal name

Bu-1b antibody

Alternative names for Bu-1b antibody

B6.2, chicken B-cell marker

SwissProt ID

Q90747 (Chick)

Ncbi ID

9031

Available reactivities

Chk (Chicken)

Available hosts

Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P)

Background of Bu-1b antibody

Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were first incubated with purified Mouse anti-Chicken Bu-1b and then counter-stained with Goat anti-Mouse IgG-FITC. Small lymphocytes were then gated using light scatter criterias and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.

AM08135PU-N

Flow cytometry: Bu-1b Antibody (5-11G2) [NBP1-28323] - 1 ug/10^6 Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using light scatter criteria and analyzed on a flow cytometer

NBP1-28323

Flow cytometry / FACS analysis: Bu-1b Antibody (5-11G2) [FITC] [NBP1-28324] - Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using lignt scatter criteria and analyzed on a FACScan® flow cytometer (BDB, San Jose, CA).

NBP1-28324

Flow cytometry / FACS analysis: Bu-1b Antibody (5-11G2) [Biotin] [NBP1-28325] - Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using lignt scatter criteria and analyzed on a FACScan® flow cytometer (BDB, San Jose, CA).

NBP1-28325

Chicken peripheral blood mononuclear cells were first incubated with Chicken Bu-1b monoclonal antibody, clone 5-11G2 ( Cat # MAB3276 ) and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using lignt scatter criteria and analyzed on a FACScan^TM flow cytometer ( BDB, San Jose, CA ).

MAB3276

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM08135BT-N	Bu-1b antibody
	Mouse	IgG1	5-11G2	Biotin	Chk	F	0.5 mg / €450.00
							OriGene Technologies GmbH
AM08135FC-N	Bu-1b antibody
	Mouse	IgG1	5-11G2	FITC	Chk	F	0.5 mg / €500.00
							OriGene Technologies GmbH
AM08135PU-N	Bu-1b antibody
	Mouse	IgG1	5-11G2	Purified	Chk	C, F, IP	0.5 mg / €370.00
							OriGene Technologies GmbH
AM08135RP-N	Bu-1b antibody
	Mouse	IgG1	5-11G2	PE	Chk	F	0.1 mg / €340.00
							OriGene Technologies GmbH

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Bu-1b antibody

Principal name

Alternative names for Bu-1b antibody

SwissProt ID

Ncbi ID

Available reactivities

Available hosts

Available applications

Background of Bu-1b antibody

Primary Antibodies

Bu-1b antibody

Bu-1b antibody

Bu-1b antibody

Bu-1b antibody

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