BUB1A antibody
Principal name
BUB1A antibody
Alternative names for BUB1A antibody
BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1
Ncbi ID
9606, NP_004327.1, NM_004336, NP_004327, O43683, NM_009772, NM_001113179
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of BUB1A antibody
The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Aphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with uligned chromosomes from prematurely entering aphase. Research studies have shown that Bub1b and Bub1 kises are mutated in several types of human maligncies including hematopoietic, colorectal, lung, and breast cancers. Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome. Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility. Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores, and is required for functiol microtubule-kinetochore interactions.
Immunofluorescence of monoclonal antibody to BUB1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M01), clone 4C3-4C9.Lane 1: BUB1 transfected lysate(122.4 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged BUB1 is approximately 0.03ng/ml as a capture antibody.
BUB1 monoclonal antibody (M02), clone 2F9 Western Blot analysis of BUB1 expression in HeLa NE ( Cat # L013V3 ).
Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M02), clone 2F9.Lane 1: BUB1 transfected lysate(122 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged BUB1 is approximately 0.1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BUB1 polyclonal antibody ( Cat # PAB3262 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of BUB1 polyclonal antibody ( Cat # PAB3262 ) in T-47D cell line lysate. BUB1 ( arrow ) was detected using the purified polyclonal antibody.
Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.Lane1:BUB1 transfected lysate(119.35 KDa).Lane2:Non-transfected lysate.
BUB1 MaxPab polyclonal antibody. Western Blot analysis of BUB1 expression in 293.
Proximity Ligation Analysis (PLA) of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in mouse testis.
Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.Lane 1: BUB1 transfected lysate(122.40 KDa).Lane 2: Non-transfected lysate.
BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in Jurkat.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of anti-BUB1A Antibody (C-term) in T47D cell line lysates (35ug/lane). BUB1A (arrow) was detected using the purified Pab.
Immunocytochemistry of BUB1 in A-20 cells with BUB1 polyclonal antibody ( Cat # PAB13270 ) at 10 µg/ml.
Western blot analysis of BUB1 in A-20 lysate with BUB1 polyclonal antibody ( Cat # PAB13270 ) at ( A ) 2 and ( B ) 4 µg/ml.
Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL.
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 ug/mL.
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 ug/mL.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COLO205 cells, using BUB1 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of HeLa cell line , using BUB1 antibody.
Western blot analysis of BUB1 expression in COLO205 cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306432 | BUB1A (N-term) antibody |
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| Rabbit Polyclonal Bub1 Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA312145 | BUB1A antibody |
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| Rabbit polyclonal anti-BUB1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312146 | BUB1A antibody |
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| Rabbit polyclonal anti-BUB1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327215 | BUB1A antibody |
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| Rabbit anti-BUB1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347945 | BUB1A antibody |
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| Rabbit Polyclonal Anti-BUB1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349726 | BUB1A antibody |
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| Rabbit Polyclonal Anti-BUB1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP08494PU-N | BUB1A antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| AP14998PU-N | BUB1A (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307013 | BUB1A |
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| Recombinant protein of human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401381 | BUB1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211557L2V | Lenti ORF particles, Bub1 (GFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2, 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,960.00
|
|
| OriGene Technologies, Inc. | ||
| MR211557L1V | Lenti ORF particles, Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,770.00
|
|
| OriGene Technologies, Inc. | ||
