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CACNA2D1 antibody

Principal name

CACNA2D1 antibody

Alternative names for CACNA2D1 antibody

CACNL2A, CCHL2A, MHS3, Voltage-dependent calcium channel subunit alpha-2/delta-1

SwissProt ID

O08532 (Mouse), P13806 (Rabit), P54289 (Human), P54290 (Rat)

Gene ID

781 (CACNA2D1), 100009105, 12293 (Cacna2d1)

Ncbi ID

NP_000713, 10116, 9606, NP_000713.2, NM_000722, NP_001075745.1, NP_001104313.1, NM_009784, NM_001110843, NM_001110844, NM_001110845, NM_001110846

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of CACNA2D1 antibody

Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

General readings

Stotz,S.C., et al., (2004) J. Biol. Chem. 279 (5), 3793-3800

Human Muscle; WB Suggested Anti-CACNA2D1 Antibody Titration: 0.12ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; CACNA2D1 antibody - middle region (ARP34952_P050) in Human Muscle cells using Western Blot

ARP34952_P050

Fetal Muscle; Host: Rabbit. Target Name:CACNA2D1. Sample Tissue:Fetal Muscle. Lane A: Primary Antibody . Lane B:Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/Lane. Gel Concentration: 0.12.; CACNA2D1 antibody - middle region (ARP34952_P050) in Fetal Muscle cells using Western Blot

ARP34952_P050

Western blot analysis using anti-CACNA2D antibody at 1.0 µg/ml on K562 cell lysate.

AP05136PU-N

CACNA2D1 polyclonal antibody ( Cat # PAB7294 ) ( 0.03 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7294

Western blot analysis of CACNA2D1 Antibody (N-term) (AP17917PU-N) in mouse brain tissue lysates (35ug/lane). CACNA2D1 (arrow) was detected using the purified Pab.

AP17917PU-N

AP17917PU-N CACNA2D1 Antibody (N-term) Flow Cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

AP17917PU-N

Nerve stained with CA2D1 antibody Cat.-No AP55391PU-N in Immunohistochemistry on Paraffin Sections

AP55391PU-N

TA303244 (0.03µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303244

WB Suggested Anti-CACNA2D1 Antibody Titration: 0.12 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle

TA338373

Host: Rabbit; Target Name:CACNA2D1; Sample Tissue:Fetal Muscle; Lane A: Primary Antibody; Lane B:Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1 ug/ml; Peptide Concentration: 5ug/ml; Lysate Quantity: 25ug/lane/Lane; Gel Concentratio

TA338373

Western blot analysis of CACNA2D1 expression in rat brain (A) whole cell lysates.

Immunohistochemical analysis of CACNA2D1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA303244	CACNA2D1 antibody
	Goat Polyclonal Antibody against CACNA2D1
	Goat		Aff - Purified	Hu	WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
AP05136PU-N	CACNA2D1 antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 mg / €400.00
						OriGene Technologies GmbH
AP17917PU-N	CACNA2D1 (N-term) antibody
	Rabbit			Hu	WB	0.4 ml / €415.00
						OriGene Technologies GmbH
+1 additional image
AP55391PU-N	CACNA2D1 (alpha-2) antibody
	Rabbit		Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €450.00
						OriGene Technologies GmbH

Lysates

Catalog No.
LY424551	CACNA2D1 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

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