CADM4 antibody
Principal name
CADM4 antibody
Alternative names for CADM4 antibody
Cell adhesion molecule 4, IGSF4C, NECL4, TSLL2, Immunoglobulin superfamily member 4C, Nectin-like protein 4, TSLC1-like protein 2
Ncbi ID
9606, NP_660339.1, NP_660339, NM_145296, NP_694752.1, NM_153112, NM_001047107
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of CADM4 antibody
- Kit Component:
- - KN302446G1, Cadm4 gRNA vector 1 in pCas-Guide vector
- KN302446G2, Cadm4 gRNA vector 2 in pCas-Guide vector
- KN302446D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Spiegel I, Adamsky K, Eshed Y, Milo R, Sabanay H, Sarig-Nadir O, Horresh I, Scherer SS, Rasband MN, Peles E.
A central role for Necl4 (SynCAM4) in Schwann cell-axon interaction and myelination.
Nat Neurosci. 2007 Jul;10(7):861-9. Epub 2007 Jun 3.
PMID: 17558405
CADM4 polyclonal antibody ( Cat # PAB7410 ) ( 0.05 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) .Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry: Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE).
Immunohistochemistry: Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE).
Western analysis of Human Brain Cerebellum Lysate
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot: Staining of Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer). B) (0.05 µg/ml) Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical analysis of CADM4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CADM4 expression in NCIH226 (A) whole cell lysates.
Immunoblot versus adult rat brain membranes (RBM) and adult mouse brain membranes from wild-type (WT) andSynCAM4 knockout (KO) mice and probed with N244/5 (left) and K89/41 (right) TC supe. Samples courtesy of Matt Rasband, Baylor College of Medicine.
Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).
Immunoblot versus adult rat brain membranes (RBM) and adult mouse brain membranes from wild-type (WT) andSynCAM4 knockout (KO) mice and probed with N244/5 (left) and K89/41 (right) TC supe. Samples courtesy of Matt Rasband, Baylor College of Medicine.
Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).
Staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). A) TA303285 (0.1µg/ml) and B) TA303307 (0.05µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). A) TA303285 (0.1µg/ml) and B) TA303307 (0.05µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from RAW264.7 cells, using CADM4 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CADM4 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using CADM4 antibody.The picture on the right is treated with the synthesized peptide.
Anti-CADM4 / NECL-4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CADM4 / NECL-4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA303285 | CADM4 antibody |
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| Goat Polyclonal Antibody against CADM4 | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA303307 | CADM4 (C-term) antibody |
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| Goat Polyclonal Antibody against CADM4 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA312478 | CADM4 antibody |
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| Rabbit polyclonal anti-CADM4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA341249 | CADM4 antibody |
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| Rabbit Polyclonal Anti-CADM4 Antibody (Internal) | |||||||
 |
Rabbit | Purified | Hu |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA351025 | CADM4 antibody |
||||||
| Rabbit Polyclonal Anti-CADM4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| 73-247 | CADM4 antibody |
||||||
 |
Mouse | IgG1 | N244/5 | Supernatant | Hu, Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
| +1 additional image |
|
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| 75-247 | CADM4 antibody |
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 |
Mouse | IgG1 | N244/5 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +1 additional image |
|
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| AP23058PU-N | CADM4 (73-83) antibody |
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 |
Goat | Aff - Purified | Bov, Bt, Can, Elephant, Eq, Hu, Mky, Ms, Platypus, Por, Rb, Rt | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP23071PU-N | CADM4 (370-384) antibody |
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 |
Goat | Aff - Purified | Bov, Bt, Can, Elephant, Eq, Hu, Mky, Por, Rb, Rt | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP313683 | CADM4 |
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| Recombinant protein of human cell adhesion molecule 4 (CADM4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403428 | CADM4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218439L2V | Lenti ORF particles, Cadm4 (GFP-tagged) - Mouse cell adhesion molecule 4 (Cadm4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR218439L1V | Lenti ORF particles, Cadm4 (Myc-DDK-tagged) - Mouse cell adhesion molecule 4 (Cadm4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214288L1V | Lenti ORF particles, Cadm4 (Myc-DDK-tagged ORF) - Rat cell adhesion molecule 4 (Cadm4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||