Calbindin antibody
Principal name
Calbindin antibody
Alternative names for Calbindin antibody
CALB1, CAB27, Calbindin D28, D-28K, Vitamin D-dependent calcium-binding protein
Ncbi ID
NP_004920.1, 9606, NP_004920, 9913, 10090, NP_001069663, NM_004929, NP_033918.1, NM_009788, NM_031984
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Radioimmunoassay (R), Immunoprecipitation (IP)
Background of Calbindin antibody
- Kit Component:
- - KN302450G1, Calb1 gRNA vector 1 in pCas-Guide vector
- KN302450G2, Calb1 gRNA vector 2 in pCas-Guide vector
- KN302450D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Parmentier, M.; Passage, E.; Vassart, G.; Mattei, M.-G. : The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3-q22.1 and 16q22-q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci. Cytogenet. Cell Genet. 57: 41-43, 1991.
Calbindin D-28 Antibody (24427)
Western Blot analysis of CALB1 expression in transfected 293T cell line (H00000793-T01) by CALB1 MaxPab polyclonal antibody.Lane 1: CALB1 transfected lysate(28.71 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab rabbit antibody to CALB1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 MaxPab rabbit polyclonal antibody.Lane 1: CALB1 transfected lysate(30.00 KDa).Lane 2: Non-transfected lysate.
Human cerebellum stained with Anti-Calbindin D28 (Cat# AP15384PU)
Western Blot analysis of kidney cell lysate with Calbindin D28 antibody
Formalin fixed paraffin embedded human kidney stained with Calbindin-D antibody Cat.-No AM08219SU (Cone CB-955)
Recombinant human Calbindin 1 , 1-261 aa: 15% SDS-PAGE (3 µg)
Immunohistochemical staining of CALB1 on formalin fixed, paraffin embedded human cerebellum with CALB1 polyclonal antibody.
Western blot analysis of Calb1 expression in rat kidney tissue lysate.
Immunoblot analysis of cell lysates.Lane 1 : IMR-32 cell lysate. Lnae 2 : Mouse brain tissue lysate.Lane 3 : SK-N-MC cell lysate.
Lane 1: Rat brain tissue lysateLane 2: Rat brain tissue lysateLane 3: Rat kidney tissue lysateLane 4: Rat lung tissue lysate
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
human purkinje fibers; CALB1 Antibody (ARP60104_P050) tested with human purkinje fibers in Immunohistochemistry; CALB1 antibody - C-terminal region (ARP60104_P050) in human purkinje fibers cells using Immunohistochemistry
Tadpole tegmenta; . Primary Antibody Dilution :. 1:500. Secondary Antibody :. Donkey anti-rabbit Alexa 488. Secondary Antibody Dilution :. 1:500. Gene Name :. CALB1. Submitted by :. Anonymous; CALB1 antibody - C-terminal region (ARP60104_P050) in Tadpole tegmenta cells using Immunohistochemistry
Tadpole tegmenta; . Primary Antibody Dilution :. 1:500. Secondary Antibody :. Donkey anti-rabbit Alexa 488. Secondary Antibody Dilution :. 1:500. Gene Name :. CALB1. Submitted by :. Anonymous; CALB1 antibody - C-terminal region (ARP60104_P050) in Tadpole tegmenta cells using Immunohistochemistry
Tadpole; . Application: IHC. Species+tissue/cell type: Tadpole tegmenta horizontal section. Primary antibody dilution: 1:500. Secondary antibody: Donkey anti-rabbit Alexa 488. Secondary antibody dilution: 1:500; CALB1 antibody - C-terminal region (ARP60104_P050) in Tadpole cells using Immunohistochemistry
Tadpole; . Species+tissue/cell type: Tadpole forebrain horizontal section. Primary antibody dilution: 1:500. Secondary antibody: Donkey anti-rabbit Alexa 488. Secondary antibody dilution: 1:500; CALB1 antibody - C-terminal region (ARP60104_P050) in Tadpole cells using Immunohistochemistry
Human COLO205; CALB1 antibody - C-terminal region (ARP60104_P050) validated by WB using COLO205 cells lysate at 1ug/ml.; CALB1 antibody - C-terminal region (ARP60104_P050) in Human COLO205 cells using Western Blot
TA311315 (0.03 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311315 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-CALB1 / Calbindin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of Calbindin-D-28K in rat kidney tissue lysate.
CALB1 Antibody (Center) (Cat. #TA325174) western blot analysis in NCI-H460 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the CALB1 antibody detected the CALB1 protein (arrow).
CALB1 antibody validated by WB using COLO205 cells lysate at 1 ug/ml.
CALB1 Antibody tested with human purkinje fibers in Immunohistochemistry
Primary Antibody Dilution: 1:500 Secondary Antibody: Donkey anti-rabbit Alexa 488 Secondary Antibody Dilution: 1:500 Gene Name: CALB1 Submitted by :Anonymous
Primary Antibody Dilution: 1:500 Secondary Antibody: Donkey anti-rabbit Alexa 488 Secondary Antibody Dilution: 1:500 Gene Name: CALB1
Species+tissue/cell type: Tadpole forebrain horizontal section; Primary antibody dilution: 1:500; Secondary antibody: Donkey anti-rabbit Alexa 488; Secondary antibody dilution: 1:500
Application: IHC; Species+tissue/cell type: Tadpole tegmenta horizontal section; Primary antibody dilution: 1:500; Secondary antibody: Donkey anti-rabbit Alexa 488; Secondary antibody dilution: 1:500
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311315 | Calbindin antibody |
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| Goat Anti-calbindin D28 Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA318675 | Calbindin antibody |
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| Anti-Calbindin Mouse Monoclonal Antibody | |||||||
 |
Mouse | IgG2a | Purified | Hu, Ms, Rt | E, IP |
50 µl /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325174 | Calbindin antibody |
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| Rabbit polyclonal CALB1 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Bov, Hu, Ms, Rt | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349732 | Calbindin antibody |
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| Rabbit Polyclonal Anti-CALB1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM20599PU-N | Calbindin antibody |
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 |
Mouse | IgG1 | CB-D7 | Aff - Purified | Rt | IP, P, WB |
0.1 mg /
€390.00
|
| OriGene Technologies GmbH | |||||||
| AP31029PU-N | Calbindin (185-199) antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.25 ml /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301358 | Calbindin |
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| Recombinant protein of human calbindin 1, 28kDa (CALB1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203429L2V | Lenti ORF particles, Calb1 (GFP-tagged) - Mouse calbindin 1 (Calb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203429L1V | Lenti ORF particles, Calb1 (Myc-DDK-tagged) - Mouse calbindin 1 (Calb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212318L2V | Lenti ORF particles, Calb1 (GFP-tagged ORF) - Rat calbindin 1 (Calb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212318L1V | Lenti ORF particles, Calb1 (Myc-DDK-tagged ORF) - Rat calbindin 1 (Calb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
