Calreticulin antibody
Principal name
Calreticulin antibody
Alternative names for Calreticulin antibody
CRP55, Calregulin, HACBP, ERp60, grp60, CALR, CRTC
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F)
Background of Calreticulin antibody
Calreticulin, also known as RO, CRT, SSA, cC1qR, FLJ26680, CALR. Entrez Protein NP_004334. It is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
General readings
Kropp LE., et al. (2010) J Immunol. 184(10):5619-27.
Singh P., et al. (2010) J Biochem. 147(5):625-32.
Human HepG2; WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. CALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; CALR antibody - C-terminal region (ARP30114_P050) in Human HepG2 cells using Western Blot
Immunoperoxidase of monoclonal antibody to CALR on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml]
Western Blot analysis of CALR expression in transfected 293T cell line by CALR monoclonal antibody (M01), clone 1G11-1A9.Lane 1: CALR transfected lysate(48 KDa).Lane 2: Non-transfected lysate.
CALR monoclonal antibody (M01), clone 1G11-1A9 Western Blot analysis of CALR expression in K-562 ( Cat # L009V1 ).
Human Daudi; WB Suggested Anti-CALR Antibody Titration: 1.0-2.0ug/ml. ELISA Titer: 1:12500. Positive Control: Daudi cell lysate. CALR is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells.; CALR antibody - C-terminal region (ARP30113_T100) in Human Daudi cells using Western Blot
Immunofluorescence of MCF-7 cells stained with antibody at 1/50 dilution.
Western Blot using antibody at 1/5,000 dilution on indicated samples.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and APP. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-APP mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CALR expression in transfected 293T cell line (H00000811-T01) by CALR MaxPab polyclonal antibody.Lane 1: CALR transfected lysate(48.10 KDa).Lane 2: Non-transfected lysate.
CALR MaxPab rabbit polyclonal antibody. Western Blot analysis of CALR expression in A-431.
Immunofluorescence staining of Calr in HCT15 colon cancer cells using Calr polyclonal antibody ( Cat # PAB12147 ). Secondary antibody was an Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder.
Western blot analysis of Calr in human kidney lysate using Calr polyclonal antibody ( Cat # PAB12148 ) at 1 : 1,000.
CALR Antibody (Center) (Cat.-No AP17172PU-N) western blot analysis in Jurkat, Ramos, Rat C6 cell line lysates (35ug/lane). This demonstrates the CALR antibody detected the CALR protein (arrow).
Anti-CALR Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST
CALR Antibody (Center) (Cat.-No AP17172PU-N). Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CALR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Recombinant human Calreticulin, 18-417 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human Brain; WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; CALR antibody - middle region (ARP58880_P050) in Human Brain cells using Western Blot
Human HepG2; WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. CALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; CALR antibody - middle region (ARP58124_P050) in Human HepG2 cells using Western Blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of CALR expression in transfected 293T cell line by CALR monoclonal antibody (M01), clone 1G11-1A9.
Western Blot analysis of CALR expression in K-562 by CALR monoclonal antibody (M01), clone 1G11-1A9.
Formalin-Fixed, Paraffin-Embedded Human Placenta stained with CALR antibody Cat.-No AM06211SU-N followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
Confocal immunofluorescence analysis of 3T3-L1 cells using Calreticulin antibody Cat.-No AM06211SU-N (green). Blue: DRAQ5 fluorescent DNA dye.
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Calreticulin antibody Cat.-No AM06211SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis using Calreticulin antibody Cat.-No AM06211SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY Calreticulin cDNA (Lane 2).
Western blot analysis using Calreticulin antibody Cat.-No AM06211SU-N against Hela (Lane 1), A549 (Lane 2), NTERA2 (Lane 3) and MCF-7 (Lane 4) cell lysate.
Immunohistochemical analysis of Calreticulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Calreticulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Calreticulin expression in HeLa (A); A431 (B); Raw264.7 (C); rat kidney (D) whole cell lysates.
Western blot analysis of Calreticulin in various cell lysates, using a 1:1000 dilution of TA309300.
Calreticulin visualized using anti-CALR, tested on Bouin's fixed paraffin-embedded backskin sections of transgenic mice.
Western blot analysis of extracts from COS-7 cells, using CALR antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using CALR antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of NIH/3T3 cells, using CALR antibody.The picture on the right is treated with the synthesized peptide.
Predicted band size: 48 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds)
Predicted cell location: Cytoplasm . Positive control: Human gastric cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using CALR antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 48 kDa . Positive control: Hela, 293T and NIH/3T3 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm . Positive control: Human gastric cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using CALR antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using IL-8R beta/CDw128 beta (Phospho-Ser347) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta (Phospho-Ser347) antibody.The picture on the right is treated with the synthesized peptide.
Anti-CALR antibody (0.01µg/ml) staining of Human (A) and Rat (B) Lung lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Calreticulin Antibody [TA336667] - Human kidney lysate.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [TA336667] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using TA336667. Secondary antibody was an Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder, Arhu
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [TA336667] - The Calreticulin antibody TA336667 was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red)
Western Blot: Calreticulin Antibody [TA336669] - Detection of calreticulin in human kidney lysate using NB 600-103 at 1:1,000. ECL exposure, 10 seconds.
Western Blot: Calreticulin Antibody [TA336669] - Western blot analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [TA336669] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (TA336669, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filam
Western Blot: Calreticulin Antibody (1G6A7) [TA336776] - Western blot analysis using anti-Calreticulin mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.
Western Blot: Calreticulin Antibody (1G6A7) [TA336776] - Western blot analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).
Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) [TA336776] - Immunohistochemical analysis of paraffin-embedded human placenta tissues using anti-Calreticulin mAb.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [TA336776] - Confocal immunofluorescence analysis of 3T3-L1 cells using anti-Calreticulin mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [TA336776] - Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-Calreticulin mAb (green). Red: Actin filaments have been labeled with DY-554 phalloi
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [TA336776] - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mou
Flow Cytometry: Calreticulin Antibody (1G6A7) [TA336776] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Calreticulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6
WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain
WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateCALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
Host: Rabbit; Target Name: CALR; Sample Tissue: 721_B Whole cell lysates; Antibody Dilution: 1.0 ug/mlThere is BioGPS gene expression data showing that CALR is expressed in 721_B
CALR Antibody (Cat.-No AP33519PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
CALR Antibody (Cat.-No AP33519PU-N) western blot analysis in Hela,SH-SY5Y,rat C6 cell line lysates (35ug/lane).This demonstrates the CALR antibody detected the CALR protein (arrow).antibody detected the CALR protein (arrow).
All lanes: Anti-CALR Antibody at 1:1000 dilution.
Lane 1: Hela whole cell lysate.
Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: Hela whole cell lysate.
Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.Blocking/Dilution buffer: 5% NFDM/TBST.
CALR Antibody (Cat.-No AP33519PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336776 | Calreticulin antibody |
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| Mouse Monoclonal Calreticulin Antibody (1G6A7) | |||||||
![Western Blot: Calreticulin Antibody (1G6A7) [TA336776] - Western blot analysis using anti-Calreticulin mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-47518-1-W.jpg) |
Mouse | IgG2a | 1G6A7 | Purified | Hu, Ms | C, E, F, ICC/IF, P, WB |
0.1 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| AM06211SU-N | Calreticulin antibody |
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Mouse | IgG2a | 1G6A7 | Ascites | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€420.00
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| OriGene Technologies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
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| AP17172PU-N | Calreticulin antibody |
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Rabbit | Aff - Purified | Hu, Rt | E, F, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AP33519PU-N | Calreticulin antibody |
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Rabbit | Aff - Purified | Hu, Rt | E, F, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA309300 | Calreticulin antibody |
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| Rabbit Polyclonal anti-CALR Antibody | |||||||
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Rabbit | IgG | Serum | Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh | C, ICC/IF, IP, WB |
0.2 ml /
€355.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312147 | Calreticulin antibody |
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| Rabbit polyclonal anti-CALR antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA321646 | Calreticulin antibody |
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| Anti-CALR Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321647 | Calreticulin antibody |
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| Anti-CALR Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA326909 | Calreticulin antibody |
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| Rabbit anti-CALR Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA327765 | Calreticulin antibody |
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| CALR Goat Polyclonal Antibody | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA336667 | Calreticulin antibody |
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| Rabbit Polyclonal Calreticulin Antibody | |||||||
![Western Blot: Calreticulin Antibody [TA336667] - Human kidney lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB600-101-1-W.jpg) |
Rabbit | Purified | Bov, Hst, Hu, Ms, Prim, Rt | C, ICC/IF, WB |
0.1 ml /
€475.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336669 | Calreticulin antibody |
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| Rabbit Polyclonal Calreticulin Antibody | |||||||
![Western Blot: Calreticulin Antibody [TA336669] - Detection of calreticulin in human kidney lysate using NB 600-103 at 1:1,000. ECL exposure, 10 seconds.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB600-103-1-W.jpg) |
Rabbit | Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM00171PU-N | Calreticulin antibody |
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Mouse | IgG1 | S75 | Purified | Hu, Mky | IP, P, WB |
0.1 mg /
€415.00
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| OriGene Technologies GmbH | |||||||
| AM31148PU-N | Calreticulin antibody |
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Mouse | IgG1 | 1G11-1A9 | Purified | Hu | E, P, WB |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP00030PU-N | Calreticulin antibody |
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Rabbit | Purified | Hu, Mky, Ms, Rt | P, WB |
0.1 mg /
€390.00
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| OriGene Technologies GmbH | |||||||
| AP05311PU-N | Calreticulin antibody |
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Sheep | IgG | Purified | Can, Hu, Ms, Rt | ICC/IF, IP, P, WB |
0.25 mg /
€400.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| SP1087P | Calreticulin antibody |
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Goat | Serum | Hu | C, WB |
0.1 ml /
€410.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303222 | Calreticulin |
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| Recombinant protein of human calreticulin (CALR) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209415L2V | Lenti ORF particles, Calr (GFP-tagged ORF) - Rat calreticulin (Calr), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR209415L1V | Lenti ORF particles, Calr (Myc-DDK-tagged ORF) - Rat calreticulin (Calr), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||