CAMK1D antibody
Principal name
CAMK1D antibody
Alternative names for CAMK1D antibody
CaM kinase I delta, Camk1D, CamKI-like protein kinase, CKLiK, CaM kinase ID, CaMKI-delta, CaM kinase ID, Calcium/calmodulin-dependent protein kinase type 1D
Ncbi ID
9606, NP_065130, NP_705718, NP_065130.1, NM_153498, NP_796317.2, NM_177343, NM_020397
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CAMK1D antibody
- Kit Component:
- - KN302475G1, Camk1d gRNA vector 1 in pCas-Guide vector
- KN302475G2, Camk1d gRNA vector 2 in pCas-Guide vector
- KN302475D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Grarup N, Andersen G, Krarup NT, Albrechtsen A, Schmitz O, Jørgensen T, Borch-Johnsen K, Hansen T, Pedersen O.
Association testing of novel type 2 diabetes risk alleles in the JAZF1, CDC123/CAMK1D, TSPAN8, THADA, ADAMTS9, and NOTCH2 loci with insulin release, insulin sensitivity, and obesity in a population-based sample of 4,516 glucose-tolerant middle-aged Danes.
Diabetes 2008 Sep 57 (9): 2534-40.
PMID: 18567820
Western Blot analysis of CAMK1D expression in transfected 293T cell line (H00057118-T01) by CAMK1D MaxPab polyclonal antibody.Lane 1: CAMK1D transfected lysate(42.35 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of CAMK1D in human brain lysate using CAMK1D polyclonal antibody ( Cat # PAB0213 ) at 2 µg/ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CAMK1D polyclonal antibody ( Cat # PAB2768 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Immunoperoxidase of monoclonal antibody to CAMK1D on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Western Blot analysis of CAMK1D expression in transfected 293T cell line by CAMK1D monoclonal antibody (M10), clone 3H8.Lane 1: CAMK1D transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CAMK1D expression in transfected 293T cell line (H00057118-T01) by CAMK1D MaxPab polyclonal antibody.Lane 1: CAMK1D transfected lysate(42.35 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CAMK1-like Antibody (C-term) (AP13881PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-CAMK1-like C-term Pab (AP13881PU-N) in NCIH460 cell lysate. CAMK1-like (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of CAMK1D expression in transfected 293T cell line (H00057118-T02) by CAMK1D MaxPab polyclonal antibody.Lane 1: CAMK1D transfected lysate(42.90 KDa).Lane 2: Non-transfected lysate.
CAMK1D polyclonal antibody ( Cat # PAB11616 ) ( 0.5 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of CAMK1D (arrow) using rabbit polyclonal CAMK1D Antibody (N-term) (AP17173PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1D gene (Lane 2).
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of CAMK1D expression in transfected 293T cell line by CAMK1D Antibody Cat.-No. AM31897PU-N
Detection limit for recombinant GST tagged CAMK1D is 0.3 ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-CAMK1Dmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500919, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CAMK1D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500919, Dilution 1:50)
Anti-CAMK1D mouse monoclonal antibody (TA500919) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CAMK1D(RC207736).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMK1D (RC207736, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMK1D.
TA305711 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of CAMK1D expression in transfected 293T cell line by CAMK1D polyclonal antibody. A: Non-transfected lysate. B: CAMK1D transfected lysate. 12% SDS PAGE. TA309025 diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Thyroid CA, using CAMK1D (TA309025) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using CaMK1D(TA309025) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. TA309026 diluted at 1:500
CaMK1D antibody detects CAMK1D protein at nucleus on breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded breast carcinoma. CaMK1D antibody (TA309026) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CAMK1D(TA309026) antibody at 1:200 dilution.
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA309027 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 . 10% SDS PAGE. TA309027 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using CAMK1D(TA309027) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CAMK1D(TA309027) antibody at 1:200 dilution.
WB Suggested Anti-CAMK1D Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: Camk1d; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CAMK1D Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (CAMK1D Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309027 | CAMK1D antibody |
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| Rabbit Polyclonal antibody to CaMK1D (calcium/calmodulin-dependent protein kinase ID) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA500919 | CAMK1D antibody |
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| anti-CAMK1D mouse monoclonal antibody, clone OTI2C6 (formerly 2C6) | |||||||
 |
Mouse | IgG1 | OTI2C6 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA305711 | CAMK1D (C-term) (Isoform 1) antibody |
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| Goat Anti-CAMK1D Antibody | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA309025 | CAMK1D antibody |
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| Rabbit polyclonal antibody to CAMK1D (calcium/calmodulin-dependent protein kinase ID) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA309026 | CAMK1D antibody |
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| Rabbit polyclonal antibody to CAMK1D (calcium/calmodulin-dependent protein kinase ID) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA349737 | CAMK1D antibody |
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| Rabbit Polyclonal Anti-CAMK1D Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AM31897PU-N | CAMK1D (1-96) antibody |
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 |
Mouse | IgG2b | 3H8 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP13881PU-N | CAMK1D (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP17173PU-N | CAMK1D (N-term) antibody |
||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307736 | CAMK1D (transcript variant 2) |
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| Recombinant protein of human calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||