CAMK2A antibody
Principal name
CAMK2A antibody
Alternative names for CAMK2A antibody
CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain
Ncbi ID
NP_741960.1, NP_741960, NP_037052.1, 9606, NP_037052, NM_171825, NP_057065.2, NP_033922.1, NM_177407, NM_009792, NM_012920, NM_015981
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Paraffin Sections (P), Immunoprecipitation (IP), Radioimmunoassay (R)
Background of CAMK2A antibody
Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning.
CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.
General readings
J Bossuyt, K Helmstadter, X Wu, et al. (2008) Ca2+/Calmodulin-Dependent Protein Kinase II {delta} and Protein Kinase D Overexpression Reinforce. Circulation Research, 102(6):695-702.
Immunofluorescence of monoclonal antibody to CAMK2A on HeLa cell. [antibody concentration 10 ug/ml]
CAMK2A monoclonal antibody (M01), clone 2C4. Western Blot analysis of CAMK2A expression in human stomach.
CAMK2A monoclonal antibody (M01), clone 2C4. Western Blot analysis of CAMK2A expression in RIN-m5F.
Detection limit for recombinant GST tagged CAMK2A is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CAMK2A is approximately 3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to CAMK2A on HeLa cell. [antibody concentration 10 ug/ml]
CAMK2A monoclonal antibody (M03), clone 2C12 Western Blot analysis of CAMK2A expression in LNCaP ( Cat # L004V1 ).
Detection limit for recombinant GST tagged CAMK2A is approximately 0.3ng/ml as a capture antibody.
Proximity Ligation Analysis (PLA) of protein-protein interactions between ACTN1 and CAMK2A. HeLa cells were stained with anti-ACTN1 rabbit purified polyclonal 1:1200 and anti-CAMK2A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Detection limit for recombinant GST tagged CAMK2A is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CAMK2A is approximately 0.1ng/ml as a capture antibody.
Figure 2. Immunofluorescence staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using CaMKII (phospho-Thr286) antibody (#AP02526PU, green).
Figure 1. Western blot analysis of extract from 293 cells untreated or treated with PMA using CaMKII (Phospho-Thr286) Antibody
Immunofluorescence staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using CaMKII antibody
Western Blot analysis of extracts from mouse brain tissue using CaMKII antibody and the same antibody preincubated with blocking peptide
Western Blot analysis of CAMK2A expression in transfected 293T cell line (H00000815-T04) by CAMK2A MaxPab polyclonal antibody.Lane 1: CAMK2A transfected lysate(52.69 KDa).Lane 2: Non-transfected lysate.
Western blot of 10 μg of rat brain lysate showing specific immunolabeling of α- and β-CaM Kinase II phosphorylated at Thr286. The labeling by the antibody to CaM Kinase II Thr286 is specifically blocked by the Thr286 phosphopeptide used as antigen. The corresponding nonphosphopeptide did not block the immunolabeling (not shown).
Western blot of 10 μg of rat brain lysate showing specific immunolabeling of α- and β-CaM Kinase II phosphorylated at Thr305. The labeling by the antibody to CaM Kinase II Thr305 is specifically blocked by the Thr305 phosphopeptide used as antigen. The corresponding nonphosphopeptide did not block the immunolabeling (not shown).
Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CAMK2A polyclonal antibody ( Cat # PAB2770 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of CAMK2A polyclonal antibody ( Cat # PAB2770 ) in HL-60 cell lysate. CAMK2A ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of CAMK2A expression in transfected 293T cell line (H00000815-T01) by CAMK2A MaxPab polyclonal antibody.Lane 1: CAMK2A transfected lysate(52.69 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2 alpha Antibody (C-term) (AP13884PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-CAMK2 alpha C-term Pab (AP13884PU-N) in 293 cell lysate. CAMK2 alpha (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2A (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining
Western blot analysis of anti-CAMK2A Antibody (C-term) in 293 cell line lysates (35ug/lane). CAMK2A(arrow) was detected using the purified Pab.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CAMK2A and SMAD2 HeLa cells were stained with anti-CAMK2A rabbit purified polyclonal 1:1200 and anti-SMAD2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of CAMK2A expression in transfected 293T cell line (H00000815-T01) by CAMK2A MaxPab polyclonal antibody.Lane 1: CAMK2A transfected lysate(52.69 KDa).Lane 2: Non-transfected lysate.
Western blot of rat brain lysate showing specific immunolabeling of the ~50 KDa- and the ~60 KDa Camk2a/Camk2b phosphorylated at Thr286 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Phospho-Camk2a/Camk2b T286. The immunolabeling is completely eliminated by treatment with l-Ptase.
Figure 1. Western blot of rat brain lysate showing specific immunolabeling of the ~50k Alpha- and the ~60k Beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE):
Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.
CAMK2A polyclonal antibody ( Cat # PAB14145 ) ( 0.1µg/ml ) staining of mouse brain lysate ( 35µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
IHC staining of hippocampal neurons
Three western blot analyses of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.
CaMKII visualized using the antibody
Immunohistochemistry (IHC) analyzes of CaMKIIalpha/delta antibody (Cat.-No.: AP20564PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of CaMKIIα/δ antibody (Cat.-No.: AP205564PU-N) in extracts from HEK293A cells.
Immunohistochemistry (IHC) analyzes of p-CaMKIIalpha/delta antibody (Cat.-No AP20855PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analyzes of p-CaMKIIalpha/delta antibody (Cat.-No AP20855PU-N) in extracts from K562 cells.
Immunohistochemistry (IHC) analyzes of p-CaMK2-a antibody (Cat.-No.: AP20879PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of p-CaMK2-a antibody (Cat.-No.: AP20879PU-N) in extracts from A431 EGF cells
Immunohistochemistry (IHC) analyzes of CaMK2-a antibody (Cat.-No.: AP20970PU-N) in paraffin-embedded human A431 tissue.
Western blot (WB) analyzes of CaMK2-a antibody (Cat.-No.: AP20970PU-N) in extracts from A431 cells.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) antibody AP55687PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2α/β/δ (Phospho-Thr305) Antibody AP55687PU-N. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of CaMK2 alpha/delta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CaMK2 alpha/delta staining in HEK293A cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CaMK2 alpha/delta expression in HEK293A (A); NIH3T3 (B) whole cell lysates.
TA305709 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2a/ß/d antibody.The picture on the right is treated with the synthesized peptide.
WB using Anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). The primary antibody diluted to 1:1,500 for 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Western blot analysis of CaMK2 alpha/delta expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Human Brain stained with anti-CaM Kinase Kinase alpha antibody.
Western blot analysis of CaMK2 alpha/ beta/ delta phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of CaMK2 phosphorylation expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from NIH/3T3 cells, using CaMKII (Phospho-Thr305) Antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2a/Ã?d (Phospho-Thr305) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) Antibody.The picture on the right is treated with the synthesized peptide.
Western blot of rat brain lysate showing specific immunolabeling of the ~50k α- and the ~60k β-CaM Kinase II phosphorylated at Thr305 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Peptide) but not by the corresponding dephosphopeptide (not shown).
Western blot of rat brain lysate showing specific immunolabeling of the ~50k α- and the ~60k β-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326521 | CamKII pThr286 antibody |
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| Mouse Monoclonal Anti-CaMKII Antibody | |||||||
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Mouse | IgG1 | 22B1 | Purified | Hu, Rt | C, E, ICC/IF, IP, WB |
0.1 mg /
€385.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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