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CAMK4 antibody

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Principal name

CAMK4 antibody

Alternative names for CAMK4 antibody

CaMK IV, CAM kinase-GR, Calcium/calmodulin-dependent protein kinase type IV

SwissProt ID

P08414 (Mouse), P13234 (Rat), Q16566 (Human)

Gene ID

814 (CAMK4), 25050 (Camk4), 12326

Ncbi ID

9606, 10090, NP_001735.1, 10116, NM_001744, NP_001735, NM_012727

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Frog, Ze (Zebrafish), Chimp (Chimpanzee), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of CAMK4 antibody

CaM Kinase IV (also known as CAM kinase-GR, CAMK4 and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampusdependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is equired for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.

General readings

Tombes R.M. et al. (1995) Cell Growth Diff. 6:1063-1070.Tokumitsu H. et al. (1995) J. Biol. Chem. 270: 19320-19324.

H00000814-M01 - CAMK4
Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml]
H00000814-M01 - CAMK4
Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10 ug/ml]
H00000814-M01 - CAMK4
Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi ( Cat # H00000814-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody (M01) clone 1A3 (Cat # H00000814-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00000814-M01 - CAMK4
CAMK4 monoclonal antibody (M01), clone 1A3 Western Blot analysis of CAMK4 expression in Jurkat ( Cat # L017V1 ).
H00000814-M01 - CAMK4
Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody (M01), clone 1A3.Lane 1: CAMK4 transfected lysate(52.14 KDa).Lane 2: Non-transfected lysate.
ARP37025_T100 - CAMK4
Human Thymus; WB Suggested Anti-CAMK4 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: Human Thymus; CAMK4 antibody - C-terminal region (ARP37025_T100) in Human Thymus cells using Western Blot
AP06503PU-N - CAMK4
Immunohistochemistry (IHC) analyzes of CaMK4 antibody (Cat.-No.: AP06503PU-N) in paraffin-embedded human breast tissue.
AP07552SU-N - CAMK4
Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE)
H00000814-B01P - CAMK4
Immunofluorescence of purified MaxPab antibody to CAMK4 on HeLa cell. [antibody concentration 10 ug/ml]
H00000814-B01P - CAMK4
Western Blot analysis of CAMK4 expression in transfected 293T cell line (H00000814-T01) by CAMK4 MaxPab polyclonal antibody.Lane 1: CAMK4 transfected lysate(52.03 KDa).Lane 2: Non-transfected lysate.
H00000814-B01P - CAMK4
CAMK4 MaxPab polyclonal antibody. Western Blot analysis of CAMK4 expression in Jurkat.
H00000814-D01P - CAMK4
Western Blot analysis of CAMK4 expression in transfected 293T cell line (H00000814-T01) by CAMK4 MaxPab polyclonal antibody.Lane 1: CAMK4 transfected lysate(51.90 KDa).Lane 2: Non-transfected lysate.
H00000814-D01P - CAMK4
CAMK4 MaxPab rabbit polyclonal antibody. Western Blot analysis of CAMK4 expression in Jurkat.
AP15388PU-S - CAMK4
Human brain stained with Anti-CaM Kinase IV, Cat. AP15388PU
PAB11208 - CAMK4
Immunohistochemical staining of Camk4 on formalin fixed, paraffin embedded human brain with Camk4 polyclonal antibody.
PAB9938 - CAMK4
Immunohistochemical staining with CAMK4 polyclonal antibody ( Cat # PAB938 ) was diluted 1 : 500 to detect CAMK4 in human brain cortex tissue.Tissue was formalin fixed and paraffin embedded.No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
PAB9938 - CAMK4
Western blot using CAMK4 polyclonal antibody ( Cat # PAB9938 ) shows detection of a band ~52 kDa corresponding to CAMK4 ( arrowhead ) in various preparations.Staining ofrat brain lysate is shown in lane 1.Jurkat cell lysate staining is shown in lane 2.Specific reactivity is blocked in both lysates when antibodyis preincubated with immunizing peptide ( lanes 3 and 4 respectively ).Approximately 35 µg of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.CAMK4 was similarly detected on lysates from mouse brain ( not shown ).After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L]MX for 45 min at room temperature.
A0831-1 - CAMK4
Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is treated with the synthesized peptide.
A0831-1 - CAMK4
Immunofluorescence analysis of HeLa cells, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is treated with the synthesized peptide.
A0831-1 - CAMK4
Western blot analysis of extracts from K562 cells treated with H2O2 100uM 30', using CaMK4 (Phospho-Thr196/200) Antibody. The lane on the right is treated with the synthesized peptide.
B0831-1 - CAMK4
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CaMK4 (Ab-196/200) Antibody. The picture on the right is treated with the synthesized peptide.
B0831-1 - CAMK4
Immunofluorescence analysis of HepG2 cells, using CaMK4 (Ab-196/200) Antibody. The picture on the right is treated with the synthesized peptide.
B0831-1 - CAMK4
Western blot analysis of extracts from K562 cells, treated with H2O2 100uM 30', using CaMK4 (Ab-196/200) Antibody. The lane on the right is treated with the synthesized peptide.
ARP61351_P050 - CAMK4
Human ACHN; WB Suggested Anti-CAMK4 Antibody. . Titration: 1.0 ug/ml. . Positive Control: ACHN Whole Cell; CAMK4 antibody - N-terminal region (ARP61351_P050) in Human ACHN cells using Western Blot
ARP61352_P050 - CAMK4
Human HepG2; WB Suggested Anti-CAMK4 Antibody. . Titration: 1.0 ug/ml. . Positive Control: HepG2 Whole Cell; CAMK4 antibody - C-terminal region (ARP61352_P050) in Human HepG2 cells using Western Blot
AM31727PU-N - CAMK4
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31727PU-N - CAMK4
Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell at 10 µg/ml.
AM31727PU-N - CAMK4
RNA interference: Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 antibody. GAPDH (36.1 kDa) used as specificity and loading control.
AR50204PU-N - CAMK4
AP55930PU-S - CAMK4
Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) antibody AP55930PU-N.The lane on the right is treated with the antigen-specific peptide.
TP306813 - CAMK4
CPA4587-100ul - CAMK4
Western blot analysis of CaMK4 (pT200) expression in Jurkat (A); Raji (B) whole cell lysates.
CPA4587-100ul - CAMK4
Immunohistochemical analysis of CaMK4 (pT200) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA4587-100ul - CAMK4
Immunofluorescent analysis of CaMK4 (pT200) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TA319475 - CAMK4
WB using Anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively).The primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000.
TA319475 - CAMK4
Anti-CAMK4 antibody was diluted 1:500 to detect CAMK4 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
TA325291 - CAMK4
Western blot analysis of CaMK4 expression in H2O2 treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA332597 - CAMK4
Western blot analysis of extracts of various cell lines, using CAMK4 antibody.
TA332597 - CAMK4
Immunohistochemistry of paraffin-embedded human kidney cancer using CAMK4 antibody at dilution of 1:200 (400x lens).
TA332597 - CAMK4
Immunohistochemistry of paraffin-embedded rat lung using CAMK4 antibody at dilution of 1:200 (400x lens).
TA332597 - CAMK4
Immunofluorescence analysis of U2OS cell using CAMK4 antibody. Blue: DAPI for nuclear staining.
TA341837 - CAMK4
WB Suggested Anti-CAMK4 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Thymus
TA341838 - CAMK4
Host: Rabbit; Target Name: Camk4; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml
TA346900 - CAMK4
Western blot detection of CaMKIV in Romas and Jurkat cell lysates using CaMKIV mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.
TA352264 - CAMK4
WB showing CaMKIV specificity. Lane 1: MW std; Lane 2: Mouse brain lysate; Lane 3: Mouse brain lysate+CIP; Lane 4: CaMKIV overexpression lysate; Lane 5: CaMKIV overexpression lysate+CIP; Lane 6: Jurkat cell lysate.
TA353631 - CAMK4
Human Brain stained with anti-CaM Kinase IV antibody.
TA349738 - CAMK4
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (CAMK4 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
TA349738 - CAMK4
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (CAMK4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
TA349738 - CAMK4
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CAMK4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
TA325292 - CAMK4
Western blot analysis of CaMK4 phosphorylation expression in H2O2 treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAMK4 antibody
  Rabbit anti-CAMK4 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CAMK4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

CAMK4 (305-323) antibody
  Rabbit polyclonal CaM Kinase IV antibody  
WB using Anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively).The primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000. Rabbit IgG Serum Bov, Can, Chimp, Chk, Frog, Hu, Ms, Rt, Ze E, P, WB
0.1 ml / €515.00
  OriGene Technologies, Inc.
+1 additional image

CAMK4 antibody
  Rabbit Polyclonal CaMK4 Antibody  
Western blot analysis of CaMK4 expression in H2O2 treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CAMK4 antibody
  Rabbit Polyclonal CaMK4 (Thr196/200) Antibody (Phospho-specific)  
Western blot analysis of CaMK4 phosphorylation expression in H2O2 treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CAMK4 antibody
  Mouse Monoclonal CaMKIV Antibody  
Western blot detection of CaMKIV in Romas and Jurkat cell lysates using CaMKIV mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa. Mouse IgG2a 3C10-D5-G7 Purified WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CAMK4 antibody
  Rabbit Polyclonal Anti-CAMK4 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (CAMK4 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

CAMK4 antibody
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1A3 Purified Hu E, ICC/IF, P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+2 additional images

CAMK4 antibody
Immunohistochemistry (IHC) analyzes of CaMK4 antibody (Cat.-No.: AP06503PU-N) in paraffin-embedded human breast tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

CAMK4 (305-323) antibody
Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Serum Bov, Can, Hu, Ms, Rt E, ICC/IF, P, WB
50 µl / €425.00
  OriGene Technologies GmbH

CAMK4 pThr196/200 antibody
Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) antibody AP55930PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €300.00
  OriGene Technologies GmbH

CAMK4 pThr196/200 antibody
Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) antibody AP55930PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €230.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CAMK4
  Recombinant protein of human calcium/calmodulin-dependent protein kinase IV (CAMK4)  
CAMK4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Camk4 (GFP-tagged ORF) - Rat calcium/calmodulin-dependent protein kinase IV (Camk4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Camk4 (GFP-tagged ORF) - Rat calcium/calmodulin-dependent protein kinase IV (Camk4), 200 uL, >10^7 TU/mL  
200 µl / €1,150.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Camk4 (Myc-DDK-tagged ORF) - Rat calcium/calmodulin-dependent protein kinase IV (Camk4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Camk4 (Myc-DDK-tagged ORF) - Rat calcium/calmodulin-dependent protein kinase IV (Camk4), 200 uL, >10^7 TU/mL  
200 µl / €1,030.00
  OriGene Technologies, Inc.
 

14 Item(s)

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14 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (11)
  2. Protein | Growth Factor (1)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (9)
  2. Monoclonal (2)
Target
  1. CAMK4 (12)
Property
  1. 305-323 (2)
  2. pThr196/200 (2)
Reactivity
  1. Human (Hu) (10)
  2. Mouse (Ms) (9)
  3. Rat (Rt) (9)
  4. Bovine (Bov) (2)
  5. Canine (Can) (2)
  6. Chimpanzee (Chimp) (1)
  7. Chicken (Chk) (1)
  8. Frog (1)
  9. Zebrafish (Ze) (1)
Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Paraffin Sections (P) (5)
  3. Enzyme Immunoassay (E) (4)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
Presentation
  1. Purified (6)
  2. Aff - Purified (3)
  3. Serum (2)
Host
  1. Rabbit (9)
  2. Mouse (2)
Images
  1. 1 image (12)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
  2. IHC (3)
  3. Paraffin Sections (P) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  5. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (14)
Service
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