CAPNS1 antibody
Principal name
CAPNS1 antibody
Alternative names for CAPNS1 antibody
Calpain small subunit 1, CSS1, CAPN4, CAPNS, Calcium-dependent protease small subunit 1, Calcium-dependent protease small subunit, Calpain regulatory subunit, Calcium-activated neutral proteinase small subunit
Ncbi ID
9606, NP_001003962, NP_001740, NP_001003962.1, NP_033925.2, NM_009795, NM_017118, NM_001003962, NM_001749
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of CAPNS1 antibody
- Kit Component:
- - KN302515G1, Capns1 gRNA vector 1 in pCas-Guide vector
- KN302515G2, Capns1 gRNA vector 2 in pCas-Guide vector
- KN302515D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Miyake S., et al. (1987) Nucleic Acids Res. 14(22): 8805-8817.Ohno S., et al. (1986) Nucleic Acids Res. 14(13): 5559.
Immunoperoxidase of monoclonal antibody to CAPNS1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to CAPNS1 on HeLa cell. [antibody concentration 20 ug/ml]
Western blot analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi ( Cat # H00000826-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody (M01), clone 3C4 (Cat # H00000826-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
CAPNS1 monoclonal antibody (M01), clone 3C4. Western Blot analysis of CAPNS1 expression in PC-12 ( Cat # L012V1 ).
CAPNS1 monoclonal antibody (M01), clone 3C4 Western Blot analysis of CAPNS1 expression in A-431 ( Cat # L015V1 ).
Western Blot analysis of CAPNS1 expression in transfected 293T cell line by CAPNS1 monoclonal antibody (M01), clone 3C4.Lane 1: CAPNS1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged CAPNS1 is approximately 0.1ng/ml as a capture antibody.
CAPNS1 MaxPab polyclonal antibody. Western Blot analysis of CAPNS1 expression in human placenta.
Western Blot analysis of CAPNS1 expression in transfected 293T cell line (H00000826-T02) by CAPNS1 MaxPab polyclonal antibody.Lane 1: CAPNS1 transfected lysate(29.48 KDa).Lane 2: Non-transfected lysate.
Human breast stained with anti-calpain antibody
Human breast carcinoma stained with anti-Calpain antibody.
Western Blot analysis of 293T cell lysate with calpain antibody
Predicted band size: 28 kDa . Positive control: Mouse spleen tissue, K562 and NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Mouse spleen tissue lysate Lane2: K562 cell lysate Lane3: NIH/3T3 cell lysate Lysates: 60 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using CAPNS1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 28 kDa . Positive control: K562 and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: K562 cell lysate Lane2: NIH/3T3 cell lysate Lysates: 60 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds)
Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using CAPNS1 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-CAPNS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lung
The recombinant human CAPNS1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: CAPNS1 Recombinant proteinLane 2.: HeLa cell lysate Lane 3.: A431 cell lysate Lane 4.: 293T cell lysate Lane 5.: Balb/3T3 cell lysateLane 6.: U87MG cell lysate
ICC/IF analysis of CAPNS1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPNS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM50627PU-N | CAPNS1 antibody |
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Mouse | IgG1 | AT1D11 | Purified | Hu | E, F, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AM50627PU-S | CAPNS1 antibody |
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|
Mouse | IgG1 | AT1D11 | Purified | Hu | E, F, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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