CAS9 antibody
Principal name
CAS9 antibody
Alternative names for CAS9 antibody
csn1, SpyCas9, CRISPR-associated endonuclease Cas9/Csn1
SwissProt ID
Gene ID
901176 (SPy_1046)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Immunoprecipitation of WT Cas9 and Cas9 nickase by using TA190309. HEK293T were transfected with either WT CAS9 plasmid (GE100002), CAS9 nickase plasmid (GE100019), or negative control vector (PS100001). Input: 10ug total cell lysates; WB was performed using TA190309 (1:2000). IP: 100ug of cell lysates were precipitated with 20ug of TA190309 or control antibody, and 5ug Protein G Beads. Mouse anti-DDK monoclonal antibody (TA50011) was used in WB to detect the immuno-precipitated Cas9 (as Cas9 is Myc-DDK tagged).
HEK293T were transfected with either WT CAS9 plasmid (GE100002), CAS9 nickase plasmid (GE100019) or control vector (PS100001). Equivalent amount of each cell lysates (10ug per lane) were immunoblotted with TA190309 (1:2000).
Western blot was performed on protein extracts from HeLa cells transfected with a FLAG-tagged Cas9 using the Cas9 antibody. The antibody was used at different dilutions. The marker is shown on the left, the position of the FLAG-tagged Cas9 protein is indicated on the right.
Western blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9 using the CRISPR/Cas9 antibody as well as two commercially available purified monoclonal myc tag antibodies (supplier A and B). The three antibodies were used at different dilutions (antibody stocks: purified monoclonal Myc A 1ug/ul, Myc B 1ug/ul and Cas9 1.9 ug/ul). The ladder has been revealed by using the HRP coupled Blue ladder antibody. Exposure time was 1 hour.
Western blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9 using the Cas9 antibody. The antibody was used at different dilutions. The position of the myc-tagged Cas9 protein is indicated on the right; the marker (in kDa) is shown on the left.
Western blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9. This figure shows the result of the WB on protein extracts from transfected (lane 2 and 4) and untransfected (lane 1 and 3) cells using the Cas9 antibody (lane 1 and 2) or an anti-myc antibody (lane 3 and 4). The marker lane (M) was incubated with Blue ladder - HRP antibody.
Hela cells were transiently transfected with a Flag-tagged Cas9 expression vector. 48 hours post transfection the cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the Cas9 (A) or with an anti- Flag (B) antibody at 4C overnight, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (bottom).
IP was performed on whole cell extracts (100 µg) from HEK293 cells transfected with a Flag-tagged Cas9 using the Cas9 antibody. The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody. Lane 3 shows the result of the IP; a negative IP control (IP on untransfected cells) and the input (15 µg) are shown in lane 2 and 1, respectively.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA190309 | CAS9 antibody |
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| Rabbit polyclonal CAS9 Antibody | |||||||
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Rabbit | Purified | S. pyogenes | WB |
0.1 ml /
€345.00
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
Top Selling product! |
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| TA160001 | CAS9 antibody |
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| Anti-CAS9 Antibody | |||||||
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Mouse | IgG1 | 7A9 | Purified | S. pyogenes | ICC/IF, IP, WB |
50 µg /
€590.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA160002 | CAS9 antibody |
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| Anti-CAS9 Antibody | |||||||
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Mouse | IgG1 | 7A9 | HRP | S. pyogenes | WB |
0.5 ml /
€620.00
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP790052 | CAS9 (N-term HIS tag) |
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| Purified recombinant protein of Bacteria S. pyogenes Cas9 endonuclease (Cas9), with N-terminal HIS tag, expressed in E. coli, 50ug | |||||
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> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€308.00
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| OriGene Technologies, Inc. | |||||
