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Caspase-4 antibody

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Principal name

Caspase-4 antibody

Alternative names for Caspase-4 antibody

CASP4, ICH2, ICE(rel)-II, Protease ICH-2, Protease TX

SwissProt ID

P49662 (Human), P70343 (Mouse), Q3MHS1 (Rat), Q5E9C1 (Bovin)

Gene ID

837 (CASP4), 12363 (Casp4), 114555

Ncbi ID

NP_001216.1, NP_031635.2, 9606, NP_001216, AAH17839, NM_001225, NM_033306, AAA86890, NM_053736

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat, Chicken, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Caspase-4 antibody

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/caspase-1, is comprised of caspase- 1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, and caspase-10. Ced- 3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the 112 kDa nuclear protein PARP into an 85 kDa apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

General readings

1. Munday NA, Vaillancourt JP, Ali A, Casano FJ, Miller DK, Molineaux SM, Yamin TT, Yu VL, Nicholson DW.
Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.J Biol Chem. 1995 Jun 30;270(26):15870-6.
PMID: 7797592.

AM00181PU-N - Caspase-4
Western blot analysis of caspase-4 in 293 cell lysate.
AP00008PU-N - Caspase-11
Western blot analysis of Caspase 11 with 3T3 cell lysate.
AP00011PU-N - Caspase-4
Western blot analysis of caspase-4 expression in HeLa cell extract.
SP2145P - Caspase-4
Immunohistochemical staining of mouse spleen with Rabbit anti caspase-4 (SP2145P)
SP2145P - Caspase-4
Western blot analysis of whole cell lysate from Ramos, human Burkitt's lymphoma derived, B-lymphoblastoid cells probed with Rabbit anti caspase-4 (SP2145P) at 0.5(A) and 1(B) µg/ml
AP07855PU-N - Caspase-4
Figure 1. Staining Caspase-4 (CASP4) in Heart by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
PAB6681 - Caspase-4
CASP4 polyclonal antibody ( Cat # PAB6681 ) ( 1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
PAB6681 - Caspase-4
CASP4 polyclonal antibody ( Cat # PAB6681 ) ( 0.3 µg/ml ) staining of human heart lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
MAB1750 - Caspase-4
Western blot analysis of CASP4 expression in 293 cell lysate.
PAB8616 - Caspase-4
Western blot analysis of Casp4 expression in NIH/3T3 cell lysate.
PAB8621 - Caspase-4
Western blot analysis of CASP4 expression in HeLa cell extract.
PAB9824 - Caspase-4
Western blot analysis of CASP4 in Ramos cells with CASP4 polyclonal antibody at ( A ) 0.5 and ( B ) 1 µg/ml.
PAB9825 - Caspase-4
Western blot analysis of CASP4 in human spleen cells with CASP4 polyclonal antibody at ( Lane 1 ) 1 and ( Lane 2 ) 2 µg/ml.
AP09034AF-N - Caspase-4
Liver: Formalin-Fixed Paraffin-Embedded (FFPE)
AP20779PU-N - Caspase-4
Immunohistochemistry (IHC) analyzes of Caspase-4 antibody (Cat.-No.: AP20779PU-N) in paraffin-embedded human lung carcinoma tissue.
AP20779PU-N - Caspase-4
Western blot (WB) analyzes of Caspase-4 antibody (Cat.-No.: AP20779PU-N) in extracts from K562 cells.
L0156-1 - Caspase-4
Western blot analysis of extracts from Jurkat cells, using Caspase 4 (p20,Cleaved-Gln81) Antibody. The lane on the right is treated with the synthesized peptide.
ARP58990_P050 - Caspase-4
Human Jurkat; WB Suggested Anti-CASP4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; CASP4 antibody - middle region (ARP58990_P050) in Human Jurkat cells using Western Blot
AP22937PU-N - Caspase-4
Immunohistochemical staining of mouse spleen using Caspase-4 antibody Ca.-No AP22937PU-N at 2 µg/ml.
AP22937PU-N - Caspase-4
Formalin-Fixed, Paraffin-Embedded Human Pancreas stained with Caspase-4 antibody Ca.-No AP22937PU-N at 5 µg/ml after heat-induced antigen retrieval.
AP22937PU-N - Caspase-4
Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 µg/ml.
AP22938PU-N - Caspase-4
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22938PU-N - Caspase-4
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22938PU-N - Caspase-4
Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody (AP22938PU-N) at (A) 1 and (B) 2 µg/ml.
AM26585AF-N - Caspase-4

Western blot analysis of Caspase-4 fragments expression in apoptosis induced SK-N-SH cells by 1 μg/mL tunicamycin using AM26585AF-N. AM26585AF-N reacts with pro-caspase-4 and cleaved form.


AM33086PU-S - Caspase-4
Western blot analysis of Raw264.7 cells starved overnight(lane 1), Raw264.7 cells starved overnight and treated with 1 ug/ml LPS for nine hours (lane 2) and NIH3T3 untreated cells (lane 3) using anti-Caspase11 antibody (clone cas11.17D9).
CPA1143-100ul - Caspase-4
Immunohistochemical analysis of Caspase 4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1143-100ul - Caspase-4
Western blot analysis of Caspase 4 expression in Ramos (A); HEK293 (B) whole cell lysates.
CPA1144-100ul - Caspase-4
Immunohistochemical analysis of Caspase 4 p20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1144-100ul - Caspase-4
Western blot analysis of Caspase 4 p20 expression in HepG2 colchicine-treated (A); SP2/0 H2O2-treated (B); rat kidney (C) whole cell lysates.
CPA3156-100ul - Caspase 4 p20
Western blot analysis of Caspase 4 p20 expression in Ramos (A) whole cell lysates.
TA805590 - Caspase-4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CASP4 (RC204711, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CASP4.(1:2000)
TA805591 - Caspase-4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CASP4 (RC204711, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CASP4.
TP760359 - Caspase-4
TA302442 - Caspase-4
TA302442 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA306195 - Caspase-4
Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 ugg/ml.
TA306195 - Caspase-4
Immunohistochemical staining of mouse spleen using caspase-4 antibody at 2 ug/ml.
TA306195 - Caspase-4
Immunofluorescence of Caspase-4 in Mouse Spleen 100 cells with Caspase-4 antibody at ug/mL.
TA306196 - Caspase-4
Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 ug/ml.
TA306196 - Caspase-4
Immunohistochemical staining of human spleen tissue using caspase-4 antibody at 2 ug/ml.
TA306196 - Caspase-4
Immunofluorescence of Caspase-4 in A20 cells with Caspase-4 antibody at 10 ug/mL.
TA326152 - Caspase-4
Western blot analysis of extracts from Jurkat cells, using CASP4 (p20, Cleaved-Gln81) antibody.The lane on the right is treated with the synthesized peptide.
TA326297 - Caspase-4
Western blot analysis of extracts from Jurkat cells, using Caspase 4/5 (p20, Cleaved-Asp270/Asp311) antibody.The lane on the right is treated with the synthesized peptide.
TA349009 - Caspase-4
Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 ug/mL.
TA349009 - Caspase-4
Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 ug/mL.

2 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Caspase-4 (N-term) antibody
  Rabbit Polyclonal Caspase-4 Antibody  
Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 ugg/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

Caspase-4 (Center) antibody
  Rabbit Polyclonal Caspase-4 Antibody  
Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

2 Item(s)

per page

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Product Type
  1. Primary Antibody (2)
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  2. N-term (1)
Reactivity
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  2. Mouse (Ms) (1)
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  2. 3+ images (2)
Images: Applications
  1. Immunofluorescence (IF) (2)
  2. IHC (2)
  3. Western blot / Immunoblot (WB) (2)
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