CASZ1 antibody
Principal name
CASZ1 antibody
Alternative names for CASZ1 antibody
CST, SRG, ZNF693, Castor-related protein, Zinc finger protein 693, Zinc finger protein castor homolog 1, Putative survival-related protein
Ncbi ID
NP_001073312, NP_001073312.1, ABZ92343.1, NP_081471.2, BC035954, NM_017766
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of CASZ1 antibody
- Kit Component:
- - KN302578G1, Casz1 gRNA vector 1 in pCas-Guide vector
- KN302578G2, Casz1 gRNA vector 2 in pCas-Guide vector
- KN302578D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot using CASZ1 polyclonal antibody ( Cat # PAB10183 ) shows detection of a band ~125 kDa in size corresponding to CASZ1 ( arrowhead ).Lane 1 contains a lysate from AS cells transfected with empty vector.Lane 2 contains lysate from AS cells transfected with CASZ1 ( predicted MW 125 kDa ).Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA ( as tested by Northern blot ).Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA.Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide ( data not shown ).Personal Communication. Carol Thiele, NCI, Bethesda, MD.
WB using anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg). The primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa).
Transfected 293T; WB Suggested Anti-CASZ1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; CASZ1 antibody - middle region (ARP33139_P050) in Transfected 293T cells using Western Blot
Human Lung (formalin-fixed, paraffin-embedded) stained with CASZ1 antibody AP22621PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
CASZ1 Antibody (C-term) (Cat. #AP50735PU-N) western blot analysis in mouse bladder tissue lysates (15µg/lane).This demonstrates the CASZ1 antibody detected CASZ1 protein (arrow).
WB using Anti-hCas5 antibody shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA.
WB Suggested Anti-CASZ1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319174 | CASZ1 antibody |
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| Rabbit polyclonal anti-Cas5 antibody | |||||||
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Rabbit | IgG | Aff - Purified | Bov, Chimp, Hu, Ms, Rt | E, WB |
0.1 mg /
€515.00
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| OriGene Technologies, Inc. | |||||||
| TA319343 | CASZ1 antibody |
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| Rabbit polyclonal anti-CASZ1 antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351981 | CASZ1 antibody |
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| Rabbit Polyclonal Anti-CASZ1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP22621PU-N | CASZ1 (N-term) antibody |
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 |
Rabbit | Aff - Purified | Bov, Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP50735PU-N | CASZ1 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206423L2V | Lenti ORF particles, Casz1 (GFP-tagged) - Mouse castor homolog 1, zinc finger (Drosophila) (cDNA clone IMAGE:4911231), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR206423L1V | Lenti ORF particles, Casz1 (Myc-DDK-tagged) - Mouse castor homolog 1, zinc finger (Drosophila) (cDNA clone IMAGE:4911231), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||