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CBFA2T2 antibody

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Principal name

CBFA2T2 antibody

Alternative names for CBFA2T2 antibody

EHT, MTGR1, MTG8-like protein, MTG8-related protein 1, ETO homologous on chromosome 20, p85, Myeloid translocation-related protein 1

SwissProt ID

O43439 (Human), O70374 (Mouse), Q96L00 (Human), Q9IAB2 (Xenla)

Gene ID

12396 (Cbfa2t2), 9139 (CBFA2T2)

Ncbi ID

NP_766448, NP_001028171, 9606, 10090, NP_005084, NM_172860, NM_001039709, NP_001034798, NP_001028171.1, NP_033953.1, NM_009823

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Sheep, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CBFA2T2 antibody

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. CBFA2T2 binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length tures of only three have been described.

General readings

Yoshikawa,T., et al., (er) Gene Expr. Patterns (2005) In press

ARP37525_P050 - CBFA2T2
Mouse NIH-3T3; WB Suggested Anti-CBFA2T2H Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; CBFA2T2H antibody - middle region (ARP37525_P050) in Mouse NIH-3T3 cells using Western Blot
H00009139-M05 - CBFA2T2
Western Blot analysis of CBFA2T2 expression in transfected 293T cell line by CBFA2T2 monoclonal antibody (M05), clone 3A2.Lane 1: CBFA2T2 transfected lysate(63.9 KDa).Lane 2: Non-transfected lysate.
H00009139-M15 - CBFA2T2
Western Blot analysis of CBFA2T2 expression in transfected 293T cell line by CBFA2T2 monoclonal antibody (M15), clone 2C10.Lane 1: CBFA2T2 transfected lysate(63.9 KDa).Lane 2: Non-transfected lysate.
H00009139-B01P - CBFA2T2
Immunofluorescence of purified MaxPab antibody to CBFA2T2 on HeLa cell. [antibody concentration 10 ug/ml]
H00009139-B01P - CBFA2T2
CBFA2T2 MaxPab polyclonal antibody. Western Blot analysis of CBFA2T2 expression in human colon.
H00009139-B01P - CBFA2T2
Western Blot analysis of CBFA2T2 expression in transfected 293T cell line (H00009139-T01) by CBFA2T2 MaxPab polyclonal antibody.Lane 1: CBFA2T2 transfected lysate(63.25 KDa).Lane 2: Non-transfected lysate.
ARP38558_P050 - CBFA2T2
Human HepG2; WB Suggested Anti-CBFA2T2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. CBFA2T2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; CBFA2T2 antibody - N-terminal region (ARP38558_P050) in Human HepG2 cells using Western Blot
ARP38559_P050 - CBFA2T2
Human Thymus; WB Suggested Anti-CBFA2T2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Thymus; CBFA2T2 antibody - middle region (ARP38559_P050) in Human Thymus cells using Western Blot
ARP37524_P050 - CBFA2T2
Mouse Brain; WB Suggested Anti-Cbfa2t2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Mouse Brain; Cbfa2t2 antibody - middle region (ARP37524_P050) in Mouse Brain cells using Western Blot
TP302013 - CBFA2T2
TA308048 - CBFA2T2
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308048 diluted at 1:1000.
TA308048 - CBFA2T2
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBFA2T2(TA308048) antibody at 1:200 dilution.
TA324594 - CBFA2T2
CBFA2T2 Antibody (N-term) (Cat. #TA324594) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CBFA2T2 antibody detected the CBFA2T2 protein (arrow).
TA324594 - CBFA2T2
IF image of Hela cell stained with CBFA2T2 Antibody (N-term)(Cat#TA324594).Hela cells were incubated with CBFA2T2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).CBFA2T2 immunoreactivity is localized to Nucleus significantly.
TA343502 - CBFA2T2
WB Suggested Anti-CBFA2T2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate; CBFA2T2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
TA343503 - CBFA2T2
WB Suggested Anti-CBFA2T2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Thymus
TA329600 - CBFA2T2
WB Suggested Anti-CBFA2T2H Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate
TA329599 - CBFA2T2
WB Suggested Anti-Cbfa2t2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Mouse Brain

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBFA2T2 antibody
  Rabbit polyclonal antibody to CBFA2T2 (core-binding factor, runt domain, alpha subunit 2; translocated to, 2)  
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308048 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

CBFA2T2 (N-term) antibody
  Rabbit polyclonal CBFA2T2 Antibody (N-term)  
CBFA2T2 Antibody (N-term) (Cat. #TA324594) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CBFA2T2 antibody detected the CBFA2T2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

2 Item(s)

per page

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  1. Primary Antibody (2)
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  2. Western blot / Immunoblot (WB) (2)
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