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CBFB antibody

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Principal name

CBFB antibody

Alternative names for CBFB antibody

Core-binding factor beta, CBF-beta, CBF beta, PEBP2-beta, PEA2-beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit

SwissProt ID

Q08024 (Mouse), Q13951 (Human)

Gene ID

865 (CBFB), 12400 (Cbfb)

Ncbi ID

NP_001746, 9606, NP_001746.1, NM_022845, NP_001154928.1, NM_022309, NM_001161456, NM_001161458, NM_001755

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of CBFB antibody

The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, alpha and beta, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2alpha subunits, PEBP2alphaA, PEBP2alphaB and PEBP2alphaC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, runt. This region is involved in DNA binding and heterodimerization with the regulatory beta subunit, which facilitates DNA binding of the alpha subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2beta is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2beta/CBFbeta-SMMHC, that is associated with human acute myeloid leukemia.

General readings

Speck NA., et al. (2009) Blood. 113(13):3070-9.
Ito Y., et al. (2008) Blood. 112(9):3777-87.

P100598_P050 - CBFB
Human K562; WB Suggested Anti-CBFB Antibody Titration: 0.2-1 ug/ml. Positive Control: K562 cell lysate; CBFB antibody - N-terminal region (P100598_P050) in Human K562 cells using Western Blot
H00000865-A01 - CBFB
CBFB polyclonal antibody (A01), Lot # 050928JC01 Western Blot analysis of CBFB expression in Jurkat ( Cat # L017V1 ).
AR09605PU-L - CBFB
AR09605PU-L - CBFB
Recombinant human CBFB, 1-182aa, His-tagged
AP20442PU-N - CBFB
Immunohistochemistry (IHC) analyzes of PEBP2beta antibody (Cat.-No.: AP202442PU-N) in paraffin-embedded human heart tissue.
AP20442PU-N - CBFB
Western blot (WB) analysis of PEBP2beta antibody (Cat.-No.: AP202442PU-N) in extracts from HUVEC
C11013-1 - CBFB
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using CBF beta Antibody. The picture on the right is treated with the synthesized peptide.
C11013-1 - CBFB
Western blot analysis of extracts from HuvEc/HeLa cells, using CBF beta Antibody. The lane on the right is treated with the synthesized peptide.
TP302111 - CBFB
TA313562 - CBFB
Western blot analysis of extracts from HeLa cells and HUVEC cells, using CBF ß antibody.The lane on the right is treated with the synthesized peptide.
TA313562 - CBFB
Immunohistochemistry analysis of paraffin-embedded human heart tissue using CBF ß antibody.The picture on the right is treated with the synthesized peptide.
TA313563 - CBFB
Western blot analysis of extracts from HepG2 cells, COLO cells, Jurkat cells, HUVEC cells and COS cells, using PPP4R1L antibody.The lane on the right is treated with the synthesized peptide.
TA320210 - CBFB
Anti-CBFB (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA324878 - CBFB
CBFB Antibody (Center) (Cat. #TA324878) western blot analysis in K562 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the CBFB antibody detected the CBFB protein (arrow).
TA324878 - CBFB
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA324878 - CBFB
Confocal immunofluorescent analysis of CBFB Antibody (Center) (Cat. #TA324878) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
TA324878 - CBFB
CBFB Antibody (Center) (Cat. #TA324878) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA330024 - CBFB
WB Suggested Anti-CBFB Antibody Titration: 0.2-1 ug/ml; Positive Control: K562 cell lysate
TA332598 - CBFB
Western blot analysis of extracts of various cell lines, using CBFB antibody.
TA332598 - CBFB
Immunofluorescence analysis of U2OS cell using CBFB antibody. Blue: DAPI for nuclear staining.
TA347086 - CBFB
ChIP assays were performed using SKNO-1 cells, the antibody against CBFb and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Image shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
TA347086 - CBFB
ChIP was performed as described above. The IP'd DNA from 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
TA347086 - CBFB
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human CBFb. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,800.
TA329190 - CBFB
Host: Rabbit; Target Name: CBFB; Sample Tissue: Jurkat Whole Cell lysates; Antibody Dilution: 1.0ug/mlCBFB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBFB antibody
  Rabbit polyclonal anti-CBF beta antibody  
Western blot analysis of extracts from HeLa cells and HUVEC cells, using CBF ß antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

CBFB antibody
  Rabbit polyclonal anti-PPP4R1L antibody  
Western blot analysis of extracts from HepG2 cells, COLO cells, Jurkat cells, HUVEC cells and COS cells, using PPP4R1L antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CBFB antibody
  Goat Polyclonal CBFB Antibody  
Anti-CBFB (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

CBFB antibody
  Rabbit polyclonal CBFB Antibody (Center)  
CBFB Antibody (Center) (Cat. #TA324878) western blot analysis in K562 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the CBFB antibody detected the CBFB protein (arrow). Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

CBFB antibody
  Rabbit anti-CBFB Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CBFB antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

CBFB antibody
  Rabbit Polyclonal CBFb Antibody  
ChIP assays were performed using SKNO-1 cells, the antibody against CBFb and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Image shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used. Rabbit IgG Purified Hu E
0.1 ml / €395.00
  OriGene Technologies, Inc.
+2 additional images

CBFB antibody
Immunohistochemistry (IHC) analyzes of PEBP2beta antibody (Cat.-No.: AP202442PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

CBFB - human gene knockout kit via CRISPR
CBFB - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CBFB - human gene knockout kit via CRISPR
CBFB - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CBFB - human gene knockout kit via CRISPR
CBFB - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CBFB - human gene knockout kit via CRISPR
CBFB - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CBFB (transcript variant 2)
  Recombinant protein of human core-binding factor, beta subunit (CBFB), transcript variant 2  
CBFB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

CBFB overexpression lysate
CBFB overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cbfb (GFP-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Cbfb (GFP-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Cbfb (Myc-DDK-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Cbfb (Myc-DDK-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

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Target
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Reactivity
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Applications
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  2. Enzyme Immunoassay (E) (4)
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