CBS antibody
Principal name
CBS antibody
Alternative names for CBS antibody
Cystathionine beta-synthase, Serine sulfhydrase, Beta-thionase
SwissProt ID
P35520 (Human)
Ncbi ID
9606, NP_000062, NP_000062.1, NM_000071, NM_001178008, NM_001178009
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CBS antibody
- Kit Component:
- - KN201755G1, CBS gRNA vector 1 in pCas-Guide vector
- KN201755G2, CBS gRNA vector 2 in pCas-Guide vector
- KN201755D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Urreizti,R., (2006) J. Hum. Genet. 51 (4), 305-313
Immunoperoxidase of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human hepatocellular carcinoma. [antibody concentration 3ug/ml]
Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi ( Cat # H00000875-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS monoclonal antibody (M01), clone 3E1 (Cat # H00000875-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of CBS expression in transfected 293T cell line by CBS monoclonal antibody (M01), clone 3E1.Lane 1: CBS transfected lysate(61 KDa).Lane 2: Non-transfected lysate.
CBS monoclonal antibody (M01), clone 3E1 Western Blot analysis of CBS expression in HeLa ( Cat # L013V1 ).
CBS monoclonal antibody (M01), clone 3E1. Western Blot analysis of CBS expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged CBS is approximately 0.1ng/ml as a capture antibody.
CBS polyclonal antibody (A01), Lot # NUS1051025QCS1 Western Blot analysis of CBS expression in K-562 ( Cat # L009V1 ).
Immunoperoxidase of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged CBS is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged CBS is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged CBS is approximately 0.03ng/ml as a capture antibody.
CBS MaxPab polyclonal antibody. Western Blot analysis of CBS expression in human liver.
Western Blot analysis of CBS expression in transfected 293T cell line (H00000875-T01) by CBS MaxPab polyclonal antibody.Lane 1: CBS transfected lysate(60.61 KDa).Lane 2: Non-transfected lysate.
CBS MaxPab rabbit polyclonal antibody. Western Blot analysis of CBS expression in mouse kidney.
CBS MaxPab rabbit polyclonal antibody. Western Blot analysis of CBS expression in human pancreas.
Western Blot analysis of CBS expression in transfected 293T cell line (H00000875-T01) by CBS MaxPab polyclonal antibody.Lane 1: CBS transfected lysate(60.60 KDa).Lane 2: Non-transfected lysate.
Human Kidney; Kidney; CBS antibody - N-terminal region (ARP45746_T100) in Human Kidney cells using Immunohistochemistry
Mouse Brain; CBS antibody - N-terminal region (ARP45746_T100) in Mouse Brain cells using Immunohistochemistry
HepG2; Host: Rabbit. Target Name:CBS. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12.; CBS antibody - N-terminal region (ARP45746_T100) in HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-CBS Antibody Titration: 1.25 ug/ml. Positive Control: HepG2 cell lysate; CBS antibody - N-terminal region (ARP45746_T100) in Human HepG2 cells using Western Blot
Human kidney; CBS antibody - N-terminal region (ARP45746_T100) in Human kidney cells using Western Blot
Western blot analysis of CBS Antibody (Center) (AP17189PU-N) in mouse kidney tissue lysates (35 µg/lane). CBS (arrow) was detected using the purified Pab.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS antibody, clone 3E1 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of CBS expression in transfected 293T cell line. Lane 1: CBS transfected lysate (61 KDa). Lane 2: Non-transfected lysate.
Western Blot analysis of CBS expression in HeLa cell lysate.
Detection limit for recombinant GST tagged CBS is approximately 0.1ng/ml as a capture antibody.
Immunoprecipitation of CBS transfected lysate using anti-CBS monoclonal antibody and Protein A Magnetic Bead.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged CBS is approximately 0.3ng/ml as a capture antibody.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged CBS is approximately 0.03ng/ml as a capture antibody.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged CBS is approximately 0.03ng/ml as a capture antibody.
CBS antibody detects CBS protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. 7.5 % SDS-PAGE. CBS antibody (TA308071) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS(TA308071) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBS(TA308071) antibody at 1:200 dilution.
CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 ug HepG2 whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of CBS protein by 4 ug CBS antibody (TA308071). 7.
Western blot analysis of extracts of mouse kidney tissue, using CBS antibody.
Immunohistochemistry of paraffin-embedded human esophagus using CBS antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CBS antibody at dilution of 1:100 (400x lens).
Immunofluorescence analysis of U2OS cell using CBS antibody. Blue: DAPI for nuclear staining.
Human kidney
WB Suggested Anti-CBS Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: CBS; Sample Tissue: HepG2; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide; Primary Antibody Concentration: 1 ug/ml; Peptide Concentration: 5 ug/ml; Lysate Quantity: 25 ug/lane/lane; Gel Concentration: 0.12
Mouse Brain
Kidney
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse pancreas tissue, human fetal liver tissue, Primary antibody: (CBS Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (CBS Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308071 | CBS antibody |
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| Rabbit Polyclonal antibody to CBS (cystathionine-beta-synthase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA327044 | CBS antibody |
||||||
| Rabbit anti-CBS Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| AM31730PU-N | CBS antibody |
||||||
 |
Mouse | IgG2a | 3E1 | Purified | Hu, Rt | E, IP, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +6 additional images |
Highly validated antibody! |
||||||
| TA349577 | CBS antibody |
||||||
| Rabbit Polyclonal Anti-CBS Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AM31731PU-N | CBS antibody |
||||||
 |
Mouse | IgG2a | 6B8 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM31732PU-N | CBS antibody |
||||||
 |
Mouse | IgG1 | 6A9 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM31733PU-N | CBS antibody |
||||||
 |
Mouse | IgG1 | 5F7 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP17189PU-N | CBS antibody |
||||||
 |
Rabbit | Hu, Ms | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301755 | CBS |
||||
| Recombinant protein of human cystathionine-beta-synthase (CBS) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||