CBX8 antibody
Principal name
CBX8 antibody
Alternative names for CBX8 antibody
Chromobox protein homolog 8, Polycomb 3 homolog, Rectachrome 1, PC3, RC1
Gene ID
57332 (CBX8)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Luminex (LMNX)
Background of CBX8 antibody
Chromobox homolog 8 (CBX8 or Pc3) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression of PcG proteins have been associated with cancer. CBX8 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain, which is 40 to 50 amino acids long.
General readings
Dietrich N, Bracken AP, Trinh E, Schjerling CK, Koseki H, Rappsilber J, Helin K, Hansen KH.
Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus.
EMBO J. 2007 Mar 21;26(6):1637-48. Epub 2007 Mar 1.
PMID: 17332741
Human HepG2; WB Suggested Anti-CBX8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; CBX8 antibody - middle region (ARP34600_P050) in Human HepG2 cells using Western Blot
Human Jurkat; WB Suggested Anti-CBX8 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; CBX8 antibody - C-terminal region (ARP39422_T100) in Human Jurkat cells using Western Blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CBX8 polyclonal antibody ( Cat # PAB4357 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
The CBX8 polyclonal antibody ( Cat # PAB4357 ) is used in Western blot to detect CBX8 in mouse kidney tissue lysate .
CBX8 polyclonal antibody ( Cat # PAB7489 ) ( 0.1 µg/ml ) staining of K-562 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of CBX8 expression in transfected 293T cell line (H00057332-T01) by CBX8 MaxPab polyclonal antibody.Lane 1: CBX8 transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The anti-CBX8 (C-term) Pab (Cat.#AP12236PU-N) is used in Western blot to detect CBX8 in mouse kidney tissue lysate.
Human COLO205; WB Suggested Anti-CBX8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: COLO205 cell lysate; CBX8 antibody - N-terminal region (ARP34599_P050) in Human COLO205 cells using Western Blot
CBX8 Antibody (Center) (Cat. #AP50746PU-N) western blot analysis in mouse liver tissue lysates (35µg/lane).This demonstrates the CBX8 antibody detected the CBX8 protein (arrow).
CBX8 Luminex Elisa with 1C7 Capture (TA600302) and 2A5 Detection (TA700302) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 1C7 Capture (TA600302) and 3D1 Detection (TA700303) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 2B2 Capture (TA600303) and 6D9 Detection (TA700304) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 2C8 Capture (TA600304) and 2A5 Detection (TA700302) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 2C8 Capture (TA600304) and 3D1 Detection (TA700303) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 5F3 Capture (TA600305) and 2A5 Detection (TA700302) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 5F3 Capture (TA600305) and 3D1 Detection (TA700303) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 6D9 Capture (TA600306) and 4H3 Detection (TA700305) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 6D9 Capture (TA600306) and 2B2 Detection (TA700306) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 6D9 Capture (TA600306) and 3A11 Detection (TA700307) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 3A11 Capture (TA600307) and 6D9 Detection (TA700304) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 4H3 Capture (TA600308) and 6D9 Detection (TA700304) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 1C7 Capture (TA600302) and 2A5 Detection (TA700302) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 2C8 Capture (TA600304) and 2A5 Detection (TA700302) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 5F3 Capture (TA600305) and 2A5 Detection (TA700302) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 1C7 Capture (TA600302) and 3D1 Detection (TA700303) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 2C8 Capture (TA600304) and 3D1 Detection (TA700303) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 5F3 Capture (TA600305) and 3D1 Detection (TA700303) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 2B2 Capture (TA600303) and 6D9 Detection (TA700304) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 3A11 Capture (TA600307) and 6D9 Detection (TA700304) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 4H3 Capture (TA600308) and 6D9 Detection (TA700304) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 6D9 Capture (TA600306) and 4H3 Detection (TA700305) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 6D9 Capture (TA600306) and 2B2 Detection (TA700306) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
CBX8 Luminex Elisa with 6D9 Capture (TA600306) and 3A11 Detection (TA700307) Antibodies. Substrate used: Recombinant Human CBX8 (LY412158)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBX8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800492)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBX8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800493)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBX8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800494)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800499)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBX8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CBX8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800569)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800569)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CBX8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800617)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800617)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CBX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800617)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBX8 (RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8.
TA303368 (0.1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 43 kDa . Positive control: 231, Hela and A549 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 231 cell lysate Lane2: Hela cell lysate Lane3: A549 cell lysate Lysates: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm, nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CBX8 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 43 kDa . Positive control: 231, Hela and A549 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 231 cell lysate Lane2: Hela cell lysate Lane3: A549 cell lysate Lysates: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CBX8 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA303368 | CBX8 antibody |
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| Goat Polyclonal Antibody against CBX8 | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA322245 | CBX8 antibody |
||||||
| Anti-CBX8 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA322246 | CBX8 antibody |
||||||
| Anti-CBX8 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP12236PU-N | CBX8 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP50746PU-N | CBX8 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
