CD22 antibody
Principal name
CD22 antibody
Alternative names for CD22 antibody
SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM, Sialic acid-binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule
Ncbi ID
9606, NP_001036782.1, NP_001762, NM_001771, NP_004235, NP_001172028, NM_001185101, NM_001043317
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CD22 antibody
CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression [1]. It is more strongly expressed on prolymphocytic leukemia and HCL than in chronic lymphocytic leukemia.
B cell lineage ALL express membrane and cytoplasmic CD22.
CD22 forms a loose complex with the BcR cell antigen receptor (BcR) [2]. The cytoplasmic domain is tyrosine phosphorylated upon ligation of the BCR and associates via SH2 domains with the tyrosine phosphatase SHP-1, the tyrosine kinase Syk and phospholipase C-g1 [3,4]. CD22 down-modulates the B cell activation threshold, presumably through its association with SHP-1 and other signaling molecules [2,3]. Mice deficient in CD22 show exaggerated antibody responses to antigen and have raised levels of autoantibodies [1,5]. CD22 can also mediate cell adhesion through its interaction with cell surface molecules bearing the appropriate sialoglycoconjugates, but only when these conjugates are not on the CD22 bearing cell itself [2,6].
General readings
Cesano A. and Gayko U. Semin. Oncol. Apr. 2003 (2): 253-257.
Surface staining of human peripheral blood cells with anti-CD22 (MEM-01) PE. Cells in the lymphocyte gate were used for analysis.
Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Ab (Cat. SM1098) using peroxidase-conjugate and DAB chromogen. Note membrane staining of B cells.
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD22: FITC (SM3085F).
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD22 (SM3085PX).
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD22: RPE (SM3085R).
Staining of human peripheral blood lymphocytes with Biotin conjugated Mouse Anti Human CD22 antibody (Cat.-No SM1787B).
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD22:FITC (SM1787F/FT).
Surface staining of human peripheral blood cells with CD22 monoclonal antibody, clone MEM - 01 ( Cat # MAB0908 ) PE. Cells in the lymphocyte gate were used for analysis.
Immunofluoscent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD22-R-PE and Rat anti-Mouse CD19-FITC (Clone 6D5). Small lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells.
Surface staining of human peripheral blood cells with CD22 monoclonal antibody, clone MEM-01 (PE) ( Cat # MAB5034 ). Cells in the lymphocyte gate were used for analysis.
Human tonsil stained with anti-CD22 antibody AP21305PU
T-1356 CD22 (B-lymphocyte cell adhesion molecule, Leu-14), mouse anti human,clone IS7 (purified monoclonal IgG1, lyophilized)
Human Brain; WB Suggested Anti-CD22 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; CD22 antibody - C-terminal region (ARP59862_P050) in Human Brain cells using Western Blot
Immunohistochemical analysis of paraffin embedded Human appendicitis section, staining in cytoplasm, DAB chromogenic reaction.
Western blotting of RAJI Cell Lysate using Anti-CD22 antibody Cat.-No AP23282PU-N
Human THP-1; WB Suggested Anti-CD22 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; CD22 antibody - N-terminal region (ARP59863_P050) in Human THP-1 cells using Western Blot
Formalin-Fixed, Paraffin-Embedded Human skin stained with CD22 antibody Cat.-No. AM10061SU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic staining of b-lymphocytes.
Representative DataClone B-ly8 (CD22) was analyzed by flow cytometry using a blood sample from a healthyvolunteer. Indirect staining was performed by adding 10 µl unlabelled monoclonal antibody to 100 µl blood sample, followed by FITC-labelled secondary antibody.
Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104).
Flow Cytometric analysis of rabbit anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104) in RAMOS (green) compare to negative control of rabbit IgG (blue)
Western blot analysis of extracts from COS7 cells (Lane 2), using BL-CAM (Phospho-Tyr807) Antibody AP55784PU-N.The lane on the left is treated with antigen-specific peptide.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD22 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD22 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD22 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD22 Antibody; followed by anti-mouse IgG PE.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD22 Antibody; followed by anti-mouse IgG PE.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506404)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506405)
Anti-CD22 mouse monoclonal antibody (TA506405) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD22(RC216939).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
Immunofluorescent staining of HeLa cells using anti-CD22 mouse monoclonal antibody (TA506407).
Anti-CD22 mouse monoclonal antibody (TA506407) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD22(RC216939).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506412)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807826)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD22 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807845)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807845)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000)
Western blot analysis of extracts from 293 cells, treated with Ca2+ (40nM, 40mins), using BL-CAM (Ab-807) antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 140 kDa . Positive control: Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
To detect hCD22 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22.
To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human CD22 (TA328278) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22.
To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (TA328277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22.
To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (TA328277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22.
Western blot analysis of extracts of various cell lines, using CD22 antibody.
Human peripheral blood lymphocytes stained with purified HIB22, followed by anti-mouse IgGs FITC
Human Tonsil stained with anti-CD22 antibody.
Flow cytometric analysis of rabbit anti-CD22 (SP104) antibody in RAMOS (green) compare to negative control of rabbit IgG (blue).
Human Tonsil stained with anti-CD22 antibody.
Human Spleen stained with anti-CD22 antibody.
Human Tonsil stained with anti-CD22 antibody.
Human Reactive Lymph Node stained with anti-CD22 antibody.
Human HK Lymphoma stained with anti-CD22 antibody.
Human Tonsil stained with anti-CD22 antibody.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA506407 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12) | |||||||
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Mouse | IgG1 | OTI1F12 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA313576 | CD22 antibody |
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| Rabbit polyclonal BL-CAM (Ab-807) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324091 | CD22 antibody |
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| Anti-CD22 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA328277 | CD22 antibody |
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| Biotinylated Anti-Human sCD22 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
50 µg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA328278 | CD22 antibody |
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| Anti-Human sCD22 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA332916 | CD22 antibody |
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| Rabbit anti-CD22 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA506404 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) | |||||||
 |
Mouse | IgG1 | OTI1F2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA506405 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI1B1 (formerly 1B1) | |||||||
 |
Mouse | IgG1 | OTI1B1 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA506412 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3) | |||||||
 |
Mouse | IgG1 | OTI4C3 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA590727 | CD22 antibody |
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| Rabbit Polyclonal CD22 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA807825 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12) | |||||||
 |
Mouse | IgG1 | OTI2B12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807826 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI2E6 (formerly 2E6) | |||||||
 |
Mouse | IgG2b | OTI2E6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA807831 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI2A4 (formerly 2A4) | |||||||
 |
Mouse | IgG1 | OTI2A4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807844 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI8E6 (formerly 8E6) | |||||||
 |
Mouse | IgG1 | OTI8E6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807845 | CD22 antibody |
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| CD22 mouse monoclonal antibody, clone OTI1E6 (formerly 1E6) | |||||||
 |
Mouse | IgG1 | OTI1E6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AM00647RP-N | CD22 antibody |
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Mouse | IgG1 | RFB-4 | PE | Hu | C, ICC/IF |
0.5 ml /
€480.00
|
| OriGene Technologies GmbH | |||||||
| AM03204AC-N | CD22 antibody |
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Mouse | IgG1 | IS7 | APC | Hu | F |
100 Tests /
€290.00
|
| OriGene Technologies GmbH | |||||||
| AM03204AF-N | CD22 antibody |
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Mouse | IgG1 | IS7 | Azide Free | Hu | F |
0.1 mg /
€210.00
|
| OriGene Technologies GmbH | |||||||
| AM03204FC-N | CD22 antibody |
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Mouse | IgG1 | IS7 | FITC | Hu | F |
100 Tests /
€260.00
|
| OriGene Technologies GmbH | |||||||
| AM03204RP-N | CD22 antibody |
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|
Mouse | IgG1 | IS7 | PE | Hu | F |
100 Tests /
€290.00
|
| OriGene Technologies GmbH | |||||||
