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CD28 antibody

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Principal name

CD28 antibody

Alternative names for CD28 antibody

TP44, T-cell-specific surface glycoprotein CD28

SwissProt ID

O02757 (Felca), P10747 (Human), P31041 (Mouse), P31042 (Rat), P31043 (Chick), P42069 (Rabit), Q28071 (Bovin)

Gene ID

25660 (Cd28), 12487, 940 (CD28), 396249

Ncbi ID

NP_037253.1, 10090, NP_006130.1, 9606, NP_990642.1, NP_006130, NP_037253, AAH93698.1, NM_006139, NP_031668.3, NM_007642, NM_013121

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human), Chk (Chicken), Prim (Primate), Rb (Rabbit), Bab (Baboon)

Available hosts

Mouse, Hamster, Rat, Rabbit, Goat

Available applications

Flow Cytometry (F), Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of CD28 antibody

Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.

General readings

1. Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993 Mar;5(3):311-5.
2. Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J. 1993 Aug 1;293 ( Pt 3):835-42.
3. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
4. Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol. 1999 Jul 1;163(1):62-70.
5. Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol. 1999 Sep 15;163(6):3511-5.
6. Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001 Jan 1;166(1):197-206.
7. Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004 May;24(9):3860-73.
8. Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004 Jul;78(13):6995-7003.

SM268B - CD28
Flow cytometry analysis
SM1087LE - CD28
Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28
SM1087P - CD28
Staining of human peripheral blood lymphocytes using Rat anti Human CD28 antibody SM1087P.
SM1087R - CD28
Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:RPE (SM1087R).
SM268F - CD28
Staining of rat thymus cells with MOUSE ANTI RAT CD28:FITC (SM268F).
SM268R - CD28
Staining of Rat lymph node cells with MOUSE ANTI RAT CD28:RPE (SM268R).
SM187BT - CD28
SM187BT staining of human peripheral blood lymphocytes.
AM08037RP-N - CD28
Immunofluoscent Staining: BALB/c thymocytes were double-stained with Hamster anti-Mouse CD28-R-PE and hamster anti-Mouse CD3e-FITC. All cells were then gated and analyzed by two-color flow cytometry on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells.
AM08038FC-N - CD28
Immunofluoscent Staining: A 50/50 mixture of the A20 B-cell lymphoma andthe EL-4 T-cell lymphoma was stained with Hamster anti-Mouse CD28-FITC. Large cellswere gated and analyzed by flow cytometry. Amount Used: 3 µg/10e6 cells.
AM08121FC-N - CD28
Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD28-FITC and Mouse anti-Chicken CD3-PE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.
AM12006AF-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified
AM12006AF-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
AM12006FC-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified
AM12006FC-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
AM12006PE-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified
AM12006PE-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
AM12006AC-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified
AM12006AC-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
A0845-1 - CD28
Western blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is treated with the synthesized peptide.
ARP59096_P050 - CD28
Human Brain; WB Suggested Anti-CD28 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; CD28 antibody - C-terminal region (ARP59096_P050) in Human Brain cells using Western Blot
AM31233AF-N - CD28
A typical staining pattern with the B-T3 monoclonal antibody of lymphocytes
AM31233PU-N - CD28
A typical staining pattern with the B-T3 monoclonal antibody of lymphocytes
AM31233FC-N - CD28
A typical staining pattern with the B-T3 monoclonal antibody of lymphocytes
AM31233RP-N - CD28
A typical staining pattern with the B-T3 monoclonal antibody of lymphocytes
AM12006PU-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.
AM12006PU-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
SM268PX - CD28
SM268FX - CD28
AM12006PP-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.
AM12006PP-N - CD28
Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
CPA1184-100ul - CD28
Western blot analysis of CD28 (pY218) expression in HeLa colchicine-treated (A); Jurkat (B); Raw264.7 colchicine-treated (C); H9C2 colchicine-treated (D) whole cell lysates.
TP700222 - CD28
TP700223 - CD28
TA311351 - CD28
(0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311383 - CD28
(0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA320257 - CD28
Staining of BALB/c thymocytes with 0.25 ug of Golden Syrian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD28 Purified (filled histogram) followed by Anti-Syrian Hamster IgG FITC . Cells in the lymphocyte gate were used for analysis.
TA320259 - CD28
Staining of Lou Rat splenocytes with 0.5 ug Mouse IgG1 Isotype Control Purified (open histogram) or 0.5 ug Anti-Rat CD28 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Total viable cells were used for analysis.
TA324885 - CD28
Western blot analysis of CD28 Antibody (C-term) (Cat. #TA324885) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.
TA324885 - CD28
Confocal immunofluorescent analysis of CD28 Antibody (C-term) (Cat. #TA324885) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
TA352723 - CD28
Human peripheral blood lymphocytes stained with CD28.2 PE
TA352361 - CD28
C57BL/6 mouse splenocytes stained with purified E18, followed by anti-mouse IgG2b FITC
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD28 antibody
CD28 Hamster IgG2 37.51 Purified Ms F, FN, IP
0.5 mg / €310.00
  OriGene Technologies GmbH
 

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CD28 (Extracell. Dom.) antibody
  Goat Anti-CD28 Antibody  
(0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

CD28 antibody
  Goat Anti-Cd28 Antibody  
(0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

CD28 antibody
  Golden Syrian Hamster Anti-Mouse CD28 Purified (100 ug)  
Staining of BALB/c thymocytes with 0.25 ug of Golden Syrian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD28 Purified (filled histogram) followed by Anti-Syrian Hamster IgG FITC . Cells in the lymphocyte gate were used for analysis. Hamster 37.51 Purified Ms F, IP, P
0.1 mg / €175.00
  OriGene Technologies, Inc.

CD28 antibody
  Mouse Anti-Human CD28 Purified (100 ug)  
CD28 Mouse CD28.2 Purified Hu F, IP, P
0.1 mg / €175.00
  OriGene Technologies, Inc.

CD28 antibody
  Mouse Anti-Rat CD28 Purified (500 ug)  
Staining of Lou Rat splenocytes with 0.5 ug Mouse IgG1 Isotype Control Purified (open histogram) or 0.5 ug Anti-Rat CD28 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Total viable cells were used for analysis. Mouse JJ319 Purified Rt F, IP
0.5 mg / €340.00
  OriGene Technologies, Inc.

CD28 antibody
CD28 Mouse IgG1 B-23 APC Hu F
100 Tests / €430.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG 37.51 APC Ms F
0.1 mg / €380.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG 37.51 Biotin Ms F
0.5 mg / €350.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG 37.51 FITC Ms F
0.5 mg / €350.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG 37.51 Low Endotoxin Ms F, FN, IP
0.5 mg / €350.00
  OriGene Technologies GmbH

CD28 antibody
Immunofluoscent Staining: BALB/c thymocytes were double-stained with Hamster anti-Mouse CD28-R-PE and hamster anti-Mouse CD3e-FITC. All cells were then gated and analyzed by two-color flow cytometry on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells. Hamster IgG 37.51 PE Ms F
0.2 mg / €380.00
  OriGene Technologies GmbH

CD28 antibody
Immunofluoscent Staining: BALB/c thymocytes were double-stained with Hamster anti-Mouse CD28-R-PE and hamster anti-Mouse CD3e-FITC. All cells were then gated and analyzed by two-color flow cytometry on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells. Hamster IgG 37.51 PE Ms F
0.1 mg / €270.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG PV-1 APC Ms F
0.1 mg / €380.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG PV-1 Biotin Ms F
0.5 mg / €350.00
  OriGene Technologies GmbH

CD28 antibody
Immunofluoscent Staining: A 50/50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma was stained with Hamster anti-Mouse CD28-FITC. Large cells were gated and analyzed by flow cytometry. Amount Used: 3 µg/10e6 cells. Hamster IgG PV-1 FITC Ms F
0.5 mg / €350.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG PV-1 Low Endotoxin Ms F, FN, IP
0.5 mg / €350.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG PV-1 Purified Ms F, FN, IP
0.5 mg / €310.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG PV-1 PE Ms F
0.2 mg / €380.00
  OriGene Technologies GmbH

CD28 antibody
CD28 Hamster IgG PV-1 PE Ms F
0.1 mg / €270.00
  OriGene Technologies GmbH

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