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CD3 antibody

Principal name

CD3 antibody

Alternative names for CD3 antibody

T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

SwissProt ID

P07766 (Human), P22646 (Mouse), P27597 (Canfa), P29328 (Sheep), Q28073 (Bovin), Q5R1Q1 (Felca), Q7YRN2 (Pig), Q95LI5 (Macfa), Q98910 (Chick), Q9TUF9 (Rabit)

Gene ID

12501 (Cd3e), 916 (CD3E), 442981, 397455, 493936

Ncbi ID

NP_031674.1, NP_000724.1, 9541, 9031, 9606, NP_001003379.1, NP_999392.1, 10090, NP_000724, NP_031674, NP_001009862.1, NM_000733

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine), Eq (Equine), Fe (Feline), Prim (Primate), Chk (Chicken), Por (Porcine), Rhesus Mky (Rhesus Monkey), Bov (Bovine), Mky (Monkey), Rb (Rabbit), Rt (Rat), Chimp (Chimpanzee), Frog, GP (Guinea Pig), Hst (Hamster), Macaque, Op (Opossum), Orang-Utan, Sh (Sheep)

Available hosts

Hamster, Mouse, Rabbit, Rat, Goat

Available applications

Functional assay (FN), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Dot blot (Dot), Radioimmunoassay (R)

Background of CD3 antibody

The antigen receptor on human T lymphocytes consists of two variable immunoglobulin-like glycoproteins, alpha and beta or TCR-gama and delta, which occur in association (noncovalently) with three invariable T3 membrane proteins (CD3-gamma, CD3-delta and CD3 epsilon). In humans two of these proteins, T3-gamma and T3-delta, are glycoproteins of relative molecular mass (Mr) 25,000 (25K) and 20,000 (20K), respectively, while the third, T3-epsilon, is a 20K non-glycosylated protein. A keystone of the antigen-specific immune response is the T-cell receptor (TCR)/CD3 complex. CD3 antigen has gamma, delta, epsilon, and theta chains. The CD3 complex consist of various chains (alfa, beta, gamma, theta, epsilon and delta) mediates signal transduction in T cell receptor cells. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The infection of CD3 positive cells with HTLV led to a decrease in CD3 antigen expression. The onset of this phenomenon coincided with a decrease of CD3γ followed by the subsequent progressive reduction in CD3δ, then CD3ε and CD3ζ mRNA. This decrease in CD3 expression is a direct result of epigenetic control regulation of CD3 genes as determined by DNAse I hyper sensitivity assay (DHS) (1).
Antibodies to CD3 epsilon chain is a glycosylated protein of 290 amino acids with a single trans-membrane spanning domain residing between 127-149 with an apparent MW of 20 kDa. The epsilon chain of CD3 complex is non-glycosylated and is involved in rapid turnover of phosphotidylserine and accumulation of intracellular Ca after antigen receptor activation on T cell. The CD3 epsilon gene consist of nine exons and three mini exons of 21, 15 and 18 bases, the epsilon gene is transcribed form weak, tissue non-specific TATA less promoter (2).
The CD3E-selective (CD3 epsilon chain) antibodies were generated against peptide form unique region of the CD3 gene that is not present in other CD3 antigen chains or other cellular proteins. The antigenic sequence was common in several species but distinct from other CD antigens.

General readings

1. Beverley, P.C.L. and Callard, R.E. (1981) Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti T-cell antibody. Eur. J. Immunol. 11: 329-334.
2. Zarkesh-Esfahani H, Pockley G, Metcalfe RA, Bidlingmaier M, Wu Z, Ajami A, et al. High-dose leptin activates human leukocytes via receptor expression on monocytes. J Immunol. 2001 Oct 15;167(8):4593-9. PubMed PMID: 11591788.
3. Kung, P. et al. (1979) Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 206: 347-9.
4. Clevers, H. et al. (1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. Eur J Immunol. 18 (5): 705-10.
5. Salmerón, A. et al. (1991) A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 147: 3047-52.
6. Hirsh, M.I. and Cohen, V. (2006) Chloroquine prevents T lymphocyte suppression induced by anthrax lethal toxin. J Infect Dis. 194: 1003-7.
7. Mahon, N.G. et al. (2002) Immunohistologic evidence of myocardial disease in apparently healthy relatives of patients with dilated cardiomyopathy. J Am Coll Cardiol. 39: 455-62.
8. Lawson, C.A. et al. (2006) Early rheumatoid arthritis is associated with a deficit in the CD4+CD25high regulatory T cell population in peripheral blood. Rheumatology (Oxford). 45: 1210-7.
9. Campana, D. et al. (1987) The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 138 (2): 648-55.
10. Battaglia, A. et al. (2003) Lymphocyte populations in human lymph nodes. Alterations in CD4+ CD25+ T regulatory cell phenotype and T-cell receptor Vbeta repertoire. Immunology. 110: 304-12.
11. McIntosh, R.S. et al. (1997) Analysis of the T cell receptor V alpha repertoire in Hashimoto'sthyroiditis: evidence for the restricted accumulation of CD8+ T cells in the absence of CD4+ T cell restriction. J Clin Endocrinol Metab. 82: 1140-6.
12. Aird, I.A. et al. (1999) Leukocytes in semen from men with spinal cord injuries. Fertil Steril. 72: 97-103.
13. Coli?, M. et al. (2006) Correlation between phenotypic characteristics of mononuclear cells isolated from human periapical lesions and their in vitro production of Th1 and Th2 cytokines. Arch Oral Biol. 51: 1120-30.
14. Oliver, B. et al. (2001) Low-dose theophylline does not exert its anti-inflammatory effects in mild asthma through upregulation of interleukin-10 in alveolar macrophages. Allergy. 56: 1087-90.
15. Guntermann, C. and Alexander, D.R. (2002) CTLA-4 suppresses proximal TCR signaling in resting human CD4(+) T cells by inhibiting ZAP-70 Tyr(319) phosphorylation: a potential role for tyrosine phosphatases. J Immunol. 168: 4420-9.
16. Baturcam, E. et al. (2014) Physical exercise reduces the expression of RANTES and its CCR5 receptor in the adipose tissue of obese humans. Mediators Inflamm. 2014: 627150.
17. Clark, E.A. et al. (1983) Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens. Immunogenetics. 18 (6): 599-615.
18. Erber, W.N. et al. (1984) Immunocytochemical detection of T and B cell populations in routine blood smears. Lancet. 1 (8385): 1042-6.
19. Maggiorella, M. et al. (1998) Detection of Infectious Simian Immunodeficiency Virus in Band T- cell lymphomas of experimentally infected macaques. Blood. 91 (9): 3103 - 3111.
20. Kumpel, B. et al. (2014) Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis. Transfusion. 54 (5): 1305-16.
21. Mjösberg, J. et al. (2009) Systemic reduction of functionally suppressive CD4dimCD25highFoxp3+ Tregs in human second trimester pregnancy is induced by progesterone and 17beta-estradiol. J Immunol. 183: 759-69.
22. Hess, C. et al. (2000) Induction of neutrophil responsiveness to myeloperoxidase antibodies by their exposure to supernatant of degranulated autologous neutrophils. Blood. 96: 2822-7.
23. Choudhuri, K. et al. (2009) Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem. 284: 26096-105.
24. Dong, D. et al. (2006) T cell receptor for antigen induces linker for activation of T cell-dependent activation of a negative signaling complex involving Dok-2, SHIP-1, and Grb-2. J Exp Med. 203: 2509-18.
25. Libri, V. et al. (2008) Jakmip1 is expressed upon T cell differentiation and has an inhibitory function in cytotoxic T lymphocytes. J Immunol. 181: 5847-56.
26. Churchman, S.M. et al. (2014) Modulation of peripheral T-cell function by interleukin-7 in rheumatoid arthritis. Arthritis Res Ther. 16 (6): 511.
27. Ward, S.T. et al. (2015) The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Br J Cancer. 112 (2): 319-28.
28. Saavedra, D. et al. (2015) Biomarkers related to immunosenescence: relationships with therapy and survival in lung cancer patients. Cancer Immunol Immunother. Nov 20. [Epub ahead of print]
30. Bhat, S.S. et al. (2016) Syntaxin 8 is required for efficient lytic granule trafficking in cytotoxic T lymphocytes. Biochim Biophys Acta. Apr 17. pii: S0167-4889(16)30103-3. [Epub ahead of print]

CL001

B/ 85.7%

CL001A

FLOW CYTOMETRIC ANALYSISDonor: BALB/cCell Concentration: 1x10e6 cellsAntibody Concentration: 0.5 μg/106 cellsIsotypic Control: Purified Hamster IgG Cell Source: A/ Thymocytes, B/ Splenic T CellsPercentage of Cells Stained Above ControlA/ 51.5%

CL001A

Cell Source: A/Thymocytes, B/Splenic T Cells Percentage of Cells Stained Above Control: A/50.2, B/87.6

CL001B

Cell Source: A/Thymocytes, B/Splenic T Cells Percentage of Cells Stained Above Control: A/50.2, B/87.6

CL001BX

Cell Source: A/Thymocytes, B/Splenic T Cells Percentage of Cells Stained Above Control: A/ 66.8 B/ 94.7

CL001F

Cell Source: A/Thymocytes, B/Splenic T Cells Percentage of Cells Stained Above Control: A/ 66.8 B/ 94.7

CL001FX

Cell Source: A/ Thymocytes, B/ Splenic T Cells

Cell Source: Spleen Percentage of cells stained above control: 44.1%

CL002B

Cell Source: Spleen Percentage of cells stained above control: 44.1%

CL002BX

Cell Source: Spleen Percentage of cells stained above control: 25.5 %

CL002F

Cell Source: Spleen Percentage of cells stained above control: 25.5 %

CL002FX

Formalin fixed paraffin embedded human tonsil stained with CD3 T Cell antibody DM112/DM112-05

DM112

Formalin fixed paraffin embedded human tonsil stained with CD3 antibody (DM422)

Formalin fixed paraffin embedded human tonsil stained with CD3 antibody. DP005

Formalin-fixed, paraffin-embedded human tonsil stained with CD3 antibody DRM005 using peroxidase-conjugate and AEC. Note cell membrane staining of T cells.

DRM005

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017APC

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017APC

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017B

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017B

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017F

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017F

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017P

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017P

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017R

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017R

Staining of mouse spleen cells with RAT ANTI MOUSE CD3 EPSILON (T3):APC

SM003APC

Staining of Mouse peripheral blood lymphocytes with Rat Anti Mouse CD3 antibody FITC conjugated

SM003F

Staining of mouse spleen with RAT ANTI MOUSE CD3

SM003PS

Staining of mouse spleen cells with RAT ANTI MOUSE CD3 EPSILON (T3):RPE

SM003R

Staining of human peripheral blood lymphocytes with Mouse ANTI Human CD3-APC

Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) Pacific Blue^TM. Cells in the lymphocyte gate were used for analysis.

SM1057B

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (UCHT1) Yb174. Gated on singlets.

SM1057B

Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) FITC.

SM1057B

SM1057F staining of human peripheral blood lymphocytes.

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD3.

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD3-RPE.

Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody Cat.-No SM1754P following heat mediated antigen retrieval in EDTA buffer pH 9.0

Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with with anti Human CD3 antibody Cat.-No SM1754P. followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

Staining of Human peripheral blood lymphocytes (permeabilised) with Rat anti Human CD3 antibody Cat.-No SM1754P.

Proximity Ligation Analysis (PLA) of protein-protein interactions between CD247 and CD3E. HeLa cells were stained with anti-CD247 rabbit purified polyclonal 1:1200 and anti-CD3E mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00000916-M04

CD3E monoclonal antibody (M04), clone 4C1 Western Blot analysis of CD3E expression in HeLa ( Cat # L013V1 ).

H00000916-M04

Detection limit for recombinant GST tagged CD3E is approximately 1ng/ml as a capture antibody.

H00000916-M04

Figure 1. Peripheral Blood Lymphocytes Stained with AM05170RP-N antibody.

AM05170RP-N

AP07139PU-N CD3e antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus.

AP07139PU-N

This antibody staining of canine peripheral blood lymphocytes.

AM05528FC-N

Staining of canine peripheral blood lymphocytes with mouse anti canine CD3

AM05528PU-L

Staining of human peripheral blood lymphocytes with Rat Anti Human CD3 (SM1754F) following permeabilisation with Leucoperm.

SM1754F

Surface staining of human peripheral blood cells as Jurkat with CD3E monoclonal antibody, clone MEM - 57 ( Cat # MAB0870 ) . Cells in the lymphocyte gate were used for analysis.

MAB0870

Immunofluorescent Staining: Cells from BALB/c mesenteric lymph nodes were double-stained with anti-Mouse CD3e-FITC and anti-Mouse CD45R/B220-R-PE (clone RA3-6B2), gated on small lymphocytes and analyzed using a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount used: < / = 3 µg/10e6.

Human tonsil stained with anti-CD3 antibody Cat.-No AP15431PU

Western Blot analysis of Jurkat cell lysate with CD3 antibody Cat.-No AP15431PU

Human tonsil stained with CD3 Antibody Cat.-No AM11102PU (Clone SP7)

Western Blot using CD3 Antibody Cat.-No AM11102PU (Clone SP7)

Flow Cytometric analysis of Rabbit anti-CD3 Antibody Cat.-No AM11102PU (Clone SP7) in Jurkats (green) compare to negative Control of Rabbit IgG (blue)

Immunofluorescent Staining: Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and stained with Mouse anti-Pig CD3e-FITC. Lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.

AM08104FC-N

Immunofluorescent Staining: Peripheral blood mononuclear cells were isolated from heparinized porcine blood on Ficoll-Hypaque density gradients and incubated with Mouse anti-Porcine CD3e-Biotin, then detected with Streptavidin-FITC. Lymphocytes were gated using light scatter criteria and analyzed on a FACScan(TM) flow cytometer (BD, San Jose, CA). Amount Used: 0.3 µg/10e6 cells.

AM08105BT-N

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD3-PE and Mouse anti-Chicken CD8a-FITC (AM08116FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.2 µg/10e6 cells.

AM08114RP-N

Flow Cytometry analysis of (a) BALL-1, (b) NALM-16 and (c) HPB-ALL stained with CD3 monoclonal antibody, clone NU-T3 ( Cat # MAB1741 ) .

MAB1741

Immunohistochemical staining of CD247 on formalin fixed, paraffin embedded human tonsil with CD3E polyclonal antibody.

PAB11227

BALB/c spleen cells were double-stained with Cd3e monoclonal antibody, clone 145-2C11 ( Cat # MAB3220 )-FITC and rat anti-mouse CD19-R-PE. Lymphocytes were gated and analyzed on a FACScan^TM flow cytometer ( BDIS, San Jose, CA ).

MAB3220

Surface staining of human peripheral blood cells with CD3 monoclonal antibody, clone MEM-57 (FITC) ( Cat # MAB4616 ). Cells in the lymphocyte gate were used for analysis.

MAB4616

Cells from BALB/c mesenteric lymph nodes were double-stained with Cd3e monoclonal antibody-FITC and anti-mouse CD45R/B220-R-PE, gated on small lymphocytes and analyzed using a FACScan^TMflow cytometer ( BDIS, San Jose, CA ).

MAB5604

T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue with CMAC dye) activated by Staphylococcal enterotoxin E in contact with a single T-cell (a Jurkat cell). Panel B is an image of the two cells, stained with an anti-CD3ζ antiserum (a 1:3,000 dilution) and anti-CD3ε, clone APA1/1 (Cat. #AM12005PU-N; 10µg/ml). Visualization of CD3ζ or CD3ε was with an anti-rabbit Alexa 488 (green) or anti-mouse Alexa 594 (red) conjugate, respectively; the yellow color is produced by co-localization of CD3ζ and CD3ε (clone APA1/1) staining. Data generously provided by Dr. Balbino Alarcon, Centro de Biologia Molecular, CSIC, Madrid, Spain.

AM12005PU-N

Jurkat cell lysate was probed with anti-CD3 epsilon AM12005PU-N (0.5μg/ml).

AM12005PU-N

Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.

AM12132PU-N

Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.

AM12132LE-N

Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.

AM12132FC-N

Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.

AM12132AC-N

Immunohistochemistry (IHC) analyzes of CD3-epsilon antibody (Cat.-No.: AP20600PU-N) in paraffin-embedded human brain tissue.

AP20600PU-N

Western blot (WB) analysis of CD3-epsilon antibody (Cat.-No.: AP20600PU-N) in extracts from K562 cells.

AP20600PU-N

Western blot analysis of CD3 epsilon expression in Jurkat cells (Lane 1), MOLT4 cells (Lane 2) and HPB-ALL cells (Lane 3) using AM20299AF-N CD3e antibody.

AM20299AF-N

Immunoprecipitation of CD3 epsilon from Jurkat cells with Hamster IgG (Lane 1) or AM20299AF-N (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM20299AF-N CD3e antibody.

AM20299AF-N

Human Brain; WB Suggested Anti-CD3E Antibody Titration: 0.125ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; CD3E antibody - middle region (ARP59097_P050) in Human Brain cells using Western Blot

ARP59097_P050

AP22689PU-N CD31 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 1/50 followed by Biotin conjugated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

AP22689PU-N

AP22689PU-N CD31 antibody staining of Formalin-Fixed Paraffin-Embedded Human Spleen at 1/50 followed by Biotin conjugated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

AP22689PU-N

Human tonsil stained with anti-CD3 antibody

AP22689PU-N

AM20601PU-N

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (UCHT1) Yb174. Gated on singlets.

SM1057AF

Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) FITC.

SM1057AF

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.

DB 082-0.5

DB 082-1

DB 082-0.1

DB 082-0.2

Immunohistochemistry on Frozen Sections: Staining of human tonsil with CD3 Antibody Cat.-No AM26052PU-N

AM26052PU-N

A typical staining pattern with the B-B11 monoclonal antibody of lymphocytes

AM31215AF-N

A typical staining pattern with the B-B11 monoclonal antibody of lymphocytes

AM31215BT-N

A typical staining pattern with the B-B11 monoclonal antibody of lymphocytes

AM31215PU-N

A typical staining pattern with the B-B11 monoclonal antibody of lymphocytes

AM31215FC-N

A typical staining pattern with the B-B11 monoclonal antibody of lymphocytes

AM31215RP-N

Surface staining of human peripheral blood cells with anti-human CD3 (Clone MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017LE

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017LE

Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.

AM06435SU-N

Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and negative control (purple).

AM06435SU-N

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162).

Western Blot analysis of Jurkat cell lysate with CD3 Antibody Cat.-No AM32443PU (Clone SP162).

Flow Cytometric analysis of Rabbit anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162) in Jurkat (green) compare to negative control of Rabbit IgG (blue).

Western blot analysis of CD3e in Human Jurkat lysate in the 1) absence and 2) presence of immunizing peptide, 3) on Mouse thymus tissue lysate probed with CD3e antibody.

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

SM3017RD5

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017RD5

Surface staining of human peripheral blood cells with anti-human CD3 FITC (SM3017F). Cells in the lymphocyte gate were used for analysis.

SM3017PP

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PerCP.

SM3017PP

Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) Pacific Blue^TM. Cells in the lymphocyte gate were used for analysis.

SM1057PP

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (UCHT1) Yb174. Gated on singlets.

SM1057PP

Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) FITC.

SM1057PP

Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.

AM12132RP-N

AM26052BT-N: human tonsil, frozen section.

AM26052BT-N

Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Human Recombinant protein CD3E(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot analysis of CD3e expression in Jurkat (A); CCRFCEM (B) whole cell lysates.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506064)

TA506064

Anti-CD3E mouse monoclonal antibody (TA506064) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E(RC208276).

TA506064

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (RC208276, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E.

TA506064

Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

TA506064

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

TA506064

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3 (RC208276, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3.(1:2000)

TA807198

Flow cytometric Analysis of live Jurkat cells, using anti-CD3 antibody(TA807198),(Red), compared to a nonspecific negative control antibody(Mouse-IGg),(Blue).(1:100)

TA807198

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescent staining of Jurkat cells using CD3E mouse monoclonal antibody (UM500048, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD3E monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

TP308276

TP700034

Staining of C57Bl/6 splenocytes with Armenian Hamster IgG Isotype Control Purified (open histogram) or Anti-Mouse CD3e Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC . Total viable cells were used for analysis.

TA320270

Staining of rat splenocytes with 0.25 ug of Mouse IgG3 Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD3 Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.

TA320271

Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control Purified (open histogram) or Anti-Human CD3 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Cells in the lymphocyte gate were used for analysis.

TA320272

Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD3 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Cells in the lymphocyte gate were used for analysis.

TA320273

Predicted band size: 23 kDa . Positive control: Molt4 and Jurkat cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Molt4 and Jurkat cell lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321191

Western blot analysis of extracts of Jurkat cell lines, using CD3E antibody.

TA327165

Immunofluorescence analysis of U2OS cell using CD3E antibody. Blue: DAPI for nuclear staining.

TA327165

Flow Cytometry: CD3 Antibody (145-2C11) [TA337189] - Analysis in Mosue splenocytes using 0.5 ug of antibody. (Red represents antibody)

TA337189

TA341374 (0.1µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA341374

C57BL/6 mouse splenocytes were stained with purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC.

TA352725

Human peripheral blood lymphocytes stained with purified HIT3a and detected with anti-mouse IgGs FITC

TA352719

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM08104BT-N	CD3 antibody
	Mouse	IgG1	PPT3	Biotin	Por	F	0.5 mg / €460.00
							OriGene Technologies GmbH
	Published recently!
SM1754F	CD3 (Cytopl. Dom.) antibody
	Rat	IgG1	CD3-12	FITC	Bov, Can, Chk, Eq, Hu, Mky, Por		0.1 mg / €490.00
							OriGene Technologies GmbH
	Published recently!
SM1754P	CD3 (Cytopl. Dom.) antibody
	Rat	IgG1	CD3-12	Purified	Can, Chk, Eq, Hu, Ms, Por	C, F, ICC/IF, P, WB	0.2 mg / €570.00
							OriGene Technologies GmbH
+2 additional images	Published recently!
SM3152P	CD3 antibody
	Mouse	IgM	MEM-92	Purified	Hu	F, FN, IP	0.1 mg / €220.00
							OriGene Technologies GmbH
	Published recently!
UM500048	CD3 antibody
	CD3E mouse monoclonal antibody,clone UMAB54
	Mouse	IgG2b	UMAB54	Purified	Hu	P	0.1 ml / €468.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
UM570048	CD3 antibody
	CD3E mouse monoclonal antibody,clone UMAB54
	Mouse	IgG2b	UMAB54	Purified	Hu	P	30 µl / €180.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA506064	CD3 antibody
	CD3E (CD3 epsilon) mouse monoclonal antibody, Clone , clone OTIE10 (formerly E10)
	Mouse	IgG2b	OTIE10	Purified	Can, Hu, Mky	ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
TA320270	CD3 antibody
	Hamster Anti-Mouse CD3e Purified (500 ug)
	Hamster		145-2C11	Purified	Ms	F, IP, P, WB	0.5 mg / €260.00
							OriGene Technologies, Inc.
TA320271	CD3 antibody
	Mouse Anti-Rat CD3 Purified (100 ug)
	Mouse		eBioG4.18 (G4.18)	Purified	Rt	F, IP, P, WB	0.1 mg / €175.00
							OriGene Technologies, Inc.
TA320272	CD3 antibody
	Mouse Anti-Human CD3 Purified (100 ug)
	Mouse		HIT3a	Purified	Hu	F	0.1 mg / €195.00
							OriGene Technologies, Inc.
TA320273	CD3 antibody
	Mouse Anti-Human CD3 Purified (100 ug)
	Mouse		UCHT1	Purified	Hu	F, P	0.1 mg / €175.00
							OriGene Technologies, Inc.
TA321191	CD3 antibody
	Anti-CD3E Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA327165	CD3 antibody
	Rabbit anti-CD3E Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA337189	CD3 antibody
	Armenian Hamster Monoclonal CD3 Antibody (145-2C11)
	Hamster	IgG	145-2C11	Purified	Ms	F	0.1 mg / €415.00
							OriGene Technologies, Inc.
TA341374	CD3 antibody
	Goat Anti-CD3E (cytoplasmic) Polyclonal Antibody
	Goat			Purified	Hu, Por, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA807198	CD3 antibody
	CD3 mouse monoclonal antibody, clone UCHT1
	Mouse	IgG1	UCHT1	Purified	Hu	F, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+1 additional image
AM05170AC-N	CD3 antibody
	Mouse	IgG1	M2AB	APC	Hu	F	100 Tests / €430.00
							OriGene Technologies GmbH
AM05528FC-N	CD3 antibody
	Mouse	IgG1	CA17.2A12	FITC	Can	F	0.1 mg / €680.00
							OriGene Technologies GmbH
AM05528PU-L	CD3 antibody
	Mouse	IgG1	CA17.2A12	Purified	Can	C, F, IP	0.1 mg / €430.00
							OriGene Technologies GmbH
AM06435SU-N	CD3 antibody
	Mouse	IgG1	4E2	Ascites	Hu	E, F, WB	0.1 ml / €380.00
							OriGene Technologies GmbH
+1 additional image

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CD3 antibody

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Primary Antibodies

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